Skin wound healing is a complex biological process.Mesenchymal stem cells(MSCs)play an important role in skin wound repair due to their multidirectional differentiation potential,hematopoietic support,promotion of ste...Skin wound healing is a complex biological process.Mesenchymal stem cells(MSCs)play an important role in skin wound repair due to their multidirectional differentiation potential,hematopoietic support,promotion of stem cell implantation,self-replication,and immune regulation.Exosomes are vesicles with diameters of 40-100 nm that contain nucleic acids,proteins,and lipids and often act as mediators of cell-to-cell communication.Currently,many clinical scientists have carried out cell-free therapy for skin wounds,especially chronic wounds,using exosomes derived from MSCs.This review focuses on the latest research progress on the mechanisms of action associated with the treatment of wound healing with exosomes derived from different MSCs,the latest research progress on the combination of exosomes and other biological or nonbiological factors for the treatment of chronic skin wounds,and the new prospects and development goals of cell-free therapy.展开更多
背景:间充质干细胞源性外泌体因其众多优点被应用于多种疾病的诊断与治疗,因此精确有效以及低成本检测出间充质干细胞源性外泌体对将来疾病的诊断和治疗至关重要。目的:对间充质干细胞源性外泌体检测方法以及最新进展做一介绍。方法:检...背景:间充质干细胞源性外泌体因其众多优点被应用于多种疾病的诊断与治疗,因此精确有效以及低成本检测出间充质干细胞源性外泌体对将来疾病的诊断和治疗至关重要。目的:对间充质干细胞源性外泌体检测方法以及最新进展做一介绍。方法:检索万方、百度学术、维普、CNKI、PubMed、Web of Science、Sinomed、Embase、Cochrane、Web of Knowledge等数据库1997年6月至2019年6月期间关于间充质干细胞源性外泌体检测方法的文献,检索词分别为"间充质干细胞""外泌体""检测方法"和"mesenchymal stem cells""exosomes""detection methods"。排除重复与相关性弱的文献,将检索到的文献进行整理,共纳入60篇文献来进行分析。结果与结论:①总结了间充质干细胞的定义、生物学特性及其旁分泌作用;②总结了间充质干细胞源性外泌体的定义、生物学特性及其在临床治疗中较好的应用前景,例如调节机体免疫功能、促进受损组织再生等;③总结了目前对间充质干细胞源性外泌体常用的检测方法以及最新研究进展;④上述研究为临床应用间充质干细胞源性外泌体治疗疾病及在组织损伤修复中发挥作用提供实验基础。展开更多
基金Supported by National Natural Science Foundation of China,No.81460293Innovation and Entrepreneurship Training Program of Nanchang University,China,No.20190402184.
文摘Skin wound healing is a complex biological process.Mesenchymal stem cells(MSCs)play an important role in skin wound repair due to their multidirectional differentiation potential,hematopoietic support,promotion of stem cell implantation,self-replication,and immune regulation.Exosomes are vesicles with diameters of 40-100 nm that contain nucleic acids,proteins,and lipids and often act as mediators of cell-to-cell communication.Currently,many clinical scientists have carried out cell-free therapy for skin wounds,especially chronic wounds,using exosomes derived from MSCs.This review focuses on the latest research progress on the mechanisms of action associated with the treatment of wound healing with exosomes derived from different MSCs,the latest research progress on the combination of exosomes and other biological or nonbiological factors for the treatment of chronic skin wounds,and the new prospects and development goals of cell-free therapy.
文摘背景:间充质干细胞源性外泌体因其众多优点被应用于多种疾病的诊断与治疗,因此精确有效以及低成本检测出间充质干细胞源性外泌体对将来疾病的诊断和治疗至关重要。目的:对间充质干细胞源性外泌体检测方法以及最新进展做一介绍。方法:检索万方、百度学术、维普、CNKI、PubMed、Web of Science、Sinomed、Embase、Cochrane、Web of Knowledge等数据库1997年6月至2019年6月期间关于间充质干细胞源性外泌体检测方法的文献,检索词分别为"间充质干细胞""外泌体""检测方法"和"mesenchymal stem cells""exosomes""detection methods"。排除重复与相关性弱的文献,将检索到的文献进行整理,共纳入60篇文献来进行分析。结果与结论:①总结了间充质干细胞的定义、生物学特性及其旁分泌作用;②总结了间充质干细胞源性外泌体的定义、生物学特性及其在临床治疗中较好的应用前景,例如调节机体免疫功能、促进受损组织再生等;③总结了目前对间充质干细胞源性外泌体常用的检测方法以及最新研究进展;④上述研究为临床应用间充质干细胞源性外泌体治疗疾病及在组织损伤修复中发挥作用提供实验基础。
