摘要
目的探讨人脐带间充质干细胞外泌体(human umbilical cord mesenchymal stem cell exosomes,hUCMSC-Exo)缓解肺动脉高压(pulmonary hypertension,PH)的潜在分子作用机制。方法对hUCMSC-Exo进行提取、鉴定及高通量测序。利用GeneCards搜索PH作用靶点,在GEO数据库下载PH相关芯片(GSE53408、GSE113439),经过批次校正后筛选出差异基因。GeneCards和GEO数据库以及hUCMSC-Exo所含miRNAs预测的靶向调控的mRNAs取交集,构建蛋白互作网络筛选出hUCMSC-Exo作用于PH的关键靶点,并进行GO和KEGG富集分析。结果测序得到hUCMSC-Exo中43个高表达的miRNAs,其中17个miRNAs可作用于PH,发现其作用机制可能与前列腺癌、HIF-1信号通路、PI3K-Akt信号通路、癌症的蛋白多糖和癌症通路等相关。结论本研究可为hUCMSC-Exo防治PH的临床研究提供新思路。
OBJECTIVE To explore the potential molecular mechanism of human umbilical cord mesenchymal stem cell exosomes(hUCMSC-Exo)in alleviating pulmonary hypertension(PH).METHODS The hUCMSC-Exo were high-throughput sequenced after extracted and identified.PH target was searched in GeneCards.PH-related chip GSE53408 and GSE113439 were downloaded from GEO databases and screened for the differential genes after batch correction.The target genes in GeneCards and GEO databases and the target regulated genes predicted by miRNAs contained in hUCMSC-Exo were used for intersection.The protein-protein interaction network was constructed to screen out the key targets of hUCMSC-Exo acting on PH,then went for GO and KEGG analysis.RESULTS Forty-three high expression miRNAs were collected after hUCMSC-Exo sequenced.A total of 17 miRNAs might affect PH through prostate cancer,HIF-1 signaling pathway,PI3K-Akt signaling pathway,proteoglycans in cancer and pathways in cancer.CONCLUSION This study provides a new idea for exploring the role of hUCMSC-Exo in alleviating PH.
作者
吴璐瑶
黎伟文
肖梦媛
邓少东
陈建英
WU Luyao;LI Weiwen;XIAO Mengyuan;DENG Shaodong;CHEN Jianying(The Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;The Second Clinical Medical College,Guangdong Medical University,Dongguan 523808,China;The First Dongguan Affiliated Hospital of Guangdong Medical University,Dongguan 523710,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2023年第4期433-442,共10页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金项目(81370242)
广东医科大学学科建设项目(4SG21229GDGFY01,4SG21008G)
广东医科大学校级大学生创新创业训练计划项目(GDMU2021117,GDMU2021156)
广东医科大学附属医院干细胞临床前研究项目(2018PSSC006)
广东医科大学青年科研培育基金项目(GDMUQ2021011)
湛江市科技计划项目(2021B01145)。
关键词
肺动脉高压
人脐带间充质干细胞外泌体
外泌体测序
生物信息学
pulmonary hypertension(PH)
human umbilical cord mesenchymal stem cell exosomes(hUCMSC-Exo)
exosome sequencing
bioinformatics
