Lateral epicondylitis is a relatively common clinical prob lem, easily recognized on palpation of the lateral protu berance on the elbow. Despite the "itis" suffix, it is no an inflammatory process. Therapeu...Lateral epicondylitis is a relatively common clinical prob lem, easily recognized on palpation of the lateral protu berance on the elbow. Despite the "itis" suffix, it is no an inflammatory process. Therapeutic approaches with topical non-steroidal anti-inflammatory drugs, cortico steroids and anesthetics have limited benefit, as would be expected if inflammation is not involved. Other ap proaches have included provision of healing cytokine from blood products or stem cells, based on the recog nition that this repetitive effort-derived disorder repre sents injury. Noting calcification/ossification of tendon attachments to the lateral epicondyle(enthesitis), dry needling, radiofrequency, shock wave treatments and surgical approaches have also been pursued. Physi ologic approaches, including manipulation, therapeuti ultrasound, phonophoresis, iontophoresis, acupuncture and exposure of the area to low level laser light, ha also had limited success. This contrasts with the benefi of a simple mechanical intervention, reducing the stres on the attachment area. This is based on displacemen of the stress by use of a thin(3/4-1 inch) band applied just distal to the epicondyle. Thin bands are required as thick bands(e.g., 2-3 inch wide) simply reduce mus cle strength, without significantly reducing stress. Thi approach appears to be associated with a failure rateless than 1%, assuming the afflicted individual modifies the activity that repeatedly stresses the epicondylar attachments.展开更多
目的研究机械牵张对心脏侧群干细胞(cardiac side population cell,CSP)增殖的作用及机制。方法通过流式细胞仪分选并培养大鼠乳鼠CSP,牵张后观察细胞的增殖。并通过胸主动脉缩窄(transverse aorta constriction,TAC)建立压力负荷小鼠模...目的研究机械牵张对心脏侧群干细胞(cardiac side population cell,CSP)增殖的作用及机制。方法通过流式细胞仪分选并培养大鼠乳鼠CSP,牵张后观察细胞的增殖。并通过胸主动脉缩窄(transverse aorta constriction,TAC)建立压力负荷小鼠模型,流式细胞仪分选后观察CSP的比例。进一步通过荧光染色以及Western blot检测CSP的LRP6磷酸化水平。结果成功分离及培养CSP,机械牵张可促进CSP增殖。体内实验表明,压力负荷可明显促进CSP比例的升高。免疫荧光及Western blot实验表明,机械牵张可明显促进CSP的LPR6磷酸化。CSP过表达LRP6可明显增强机械牵张介导的促增殖效应。结论机械牵张可促进LRP6磷酸化,从而促进CSP增殖,有利于机体对病理性刺激产生适应性反应以维持心功能。展开更多
文摘Lateral epicondylitis is a relatively common clinical prob lem, easily recognized on palpation of the lateral protu berance on the elbow. Despite the "itis" suffix, it is no an inflammatory process. Therapeutic approaches with topical non-steroidal anti-inflammatory drugs, cortico steroids and anesthetics have limited benefit, as would be expected if inflammation is not involved. Other ap proaches have included provision of healing cytokine from blood products or stem cells, based on the recog nition that this repetitive effort-derived disorder repre sents injury. Noting calcification/ossification of tendon attachments to the lateral epicondyle(enthesitis), dry needling, radiofrequency, shock wave treatments and surgical approaches have also been pursued. Physi ologic approaches, including manipulation, therapeuti ultrasound, phonophoresis, iontophoresis, acupuncture and exposure of the area to low level laser light, ha also had limited success. This contrasts with the benefi of a simple mechanical intervention, reducing the stres on the attachment area. This is based on displacemen of the stress by use of a thin(3/4-1 inch) band applied just distal to the epicondyle. Thin bands are required as thick bands(e.g., 2-3 inch wide) simply reduce mus cle strength, without significantly reducing stress. Thi approach appears to be associated with a failure rateless than 1%, assuming the afflicted individual modifies the activity that repeatedly stresses the epicondylar attachments.
文摘目的研究机械牵张对心脏侧群干细胞(cardiac side population cell,CSP)增殖的作用及机制。方法通过流式细胞仪分选并培养大鼠乳鼠CSP,牵张后观察细胞的增殖。并通过胸主动脉缩窄(transverse aorta constriction,TAC)建立压力负荷小鼠模型,流式细胞仪分选后观察CSP的比例。进一步通过荧光染色以及Western blot检测CSP的LRP6磷酸化水平。结果成功分离及培养CSP,机械牵张可促进CSP增殖。体内实验表明,压力负荷可明显促进CSP比例的升高。免疫荧光及Western blot实验表明,机械牵张可明显促进CSP的LPR6磷酸化。CSP过表达LRP6可明显增强机械牵张介导的促增殖效应。结论机械牵张可促进LRP6磷酸化,从而促进CSP增殖,有利于机体对病理性刺激产生适应性反应以维持心功能。
