Objective To explore Effects of marine collagen peptides （MCPs） on markers of metablic nuclear receptors, i.e peroxisome proliferator-activated receptor （PPARs）, liver X receptor （LXRs） and farnesoid X receptor ...Objective To explore Effects of marine collagen peptides （MCPs） on markers of metablic nuclear receptors, i.e peroxisome proliferator-activated receptor （PPARs）, liver X receptor （LXRs） and farnesoid X receptor （FXRs） in type 2 diabetic patients with/without hypertension. Method Study population consisted of 200 type 2 diabetic patients with/without hypertension and 50 healthy subjects, all of whom were randomly assigned to MCPs-treated diabetics （n=50）, placebo-treated diabetics （n=50）, MCPs-treated diabetics with hypertension （n=50）, placebo-treated diabetics with hypertension （n=50）, and healthy controls （n=50）. MCPs or placebo （water-soluble starch） were given daily before breakfast and bedtime over three months. Levels of free fatty acid, cytochrome P450, leptin, resistin, adiponectin, bradykinin, NO, and Prostacyclin were determined before intervention, and 1.5 months, and 3 months after intervention. Hypoglycemia and the endpoint events during the study were recorded and compared among the study groups. Result At the end of the study period, MCPs-treated patients showed marked improvement compared with patients receiving placebo. The protection exerted by MCPs seemed more profound in diabetics than in diabetics with hypertension. In particular, after MCPs intervention, levels of free fatty acid, hs-CRP, resistin, Prostacyclin decreased significantly in diabetics and tended to decrease in diabetic and hypertensive patients whereas levels of cytochrome P450, leptin, NO tended to decrease in diabetics with/without hypertension. Meanwhile, levels of adiponectin and bradykinin rose markedly in diabetics following MCPs administration. Conclusion MCPs could offer protection against diabetes and hypertension by affecting levels of molecules involved in diabetic and hypertensive pathogenesis. Regulation on metabolic nuclear receptors by MCPs may be the possible underlying mechanism for its observed effects in the study. Further study into its action may shed light on developm展开更多
以羟自由基(OH·)、超氧阴离子自由基(O-2·)的清除率和酪氨酸酶抑制率为研究对象,研究从富含胶原的海产品下脚料中提取的海洋活性胶原肽(Marine active collagen peptides/MACP)的体外活性,并进行初步分离。结果表明:MACP的OH&...以羟自由基(OH·)、超氧阴离子自由基(O-2·)的清除率和酪氨酸酶抑制率为研究对象,研究从富含胶原的海产品下脚料中提取的海洋活性胶原肽(Marine active collagen peptides/MACP)的体外活性,并进行初步分离。结果表明:MACP的OH·清除率(IC501.42mg/mL)高于谷胱甘肽(IC508.92mg/mL)和VC,低于抗氧化剂TBHQ(IC500.375mg/mL)和茶多酚(IC500.382mg/mL);MACP的O-2·清除活性(IC5014.40mg/mL)低于茶多酚(IC504.05mg/mL)和谷胱甘肽(IC500.49mg/mL),却高于TBHQ(IC5017.52mg/mL)。MACP抑制酪氨酸酶的IC50为17.06mg/mL,为常用美白剂——熊果苷的1/70。酶解液经截流相对分子质量分别为10000、4000、1000的超滤膜分离,其活性随相对分子质量的减小而增大,且清除自由基和抑制酪氨酸酶的最大活性相对分子质量都集中在1000以下。对相对分子质量小于1000的MACP成分进行葡聚糖凝胶DEAE-A25阴离子交换分离,得到2个洗脱峰,且活性成分主要集中在第1个峰上。将MACP应用于功能食品或美白化妆品中开发前景良好。展开更多
基金grants from the National Key Technology R&D Program (No. 2006BAD27B01)Chinese Center for Disease Control and Prevention Dalone Foundation of Dietary Nutrition (No. DIC-200710)a grant from Shenzhen Bureau of Science Technology & Information (No. 200802002)
文摘Objective To explore Effects of marine collagen peptides （MCPs） on markers of metablic nuclear receptors, i.e peroxisome proliferator-activated receptor （PPARs）, liver X receptor （LXRs） and farnesoid X receptor （FXRs） in type 2 diabetic patients with/without hypertension. Method Study population consisted of 200 type 2 diabetic patients with/without hypertension and 50 healthy subjects, all of whom were randomly assigned to MCPs-treated diabetics （n=50）, placebo-treated diabetics （n=50）, MCPs-treated diabetics with hypertension （n=50）, placebo-treated diabetics with hypertension （n=50）, and healthy controls （n=50）. MCPs or placebo （water-soluble starch） were given daily before breakfast and bedtime over three months. Levels of free fatty acid, cytochrome P450, leptin, resistin, adiponectin, bradykinin, NO, and Prostacyclin were determined before intervention, and 1.5 months, and 3 months after intervention. Hypoglycemia and the endpoint events during the study were recorded and compared among the study groups. Result At the end of the study period, MCPs-treated patients showed marked improvement compared with patients receiving placebo. The protection exerted by MCPs seemed more profound in diabetics than in diabetics with hypertension. In particular, after MCPs intervention, levels of free fatty acid, hs-CRP, resistin, Prostacyclin decreased significantly in diabetics and tended to decrease in diabetic and hypertensive patients whereas levels of cytochrome P450, leptin, NO tended to decrease in diabetics with/without hypertension. Meanwhile, levels of adiponectin and bradykinin rose markedly in diabetics following MCPs administration. Conclusion MCPs could offer protection against diabetes and hypertension by affecting levels of molecules involved in diabetic and hypertensive pathogenesis. Regulation on metabolic nuclear receptors by MCPs may be the possible underlying mechanism for its observed effects in the study. Further study into its action may shed light on developm
文摘以羟自由基(OH·)、超氧阴离子自由基(O-2·)的清除率和酪氨酸酶抑制率为研究对象,研究从富含胶原的海产品下脚料中提取的海洋活性胶原肽(Marine active collagen peptides/MACP)的体外活性,并进行初步分离。结果表明:MACP的OH·清除率(IC501.42mg/mL)高于谷胱甘肽(IC508.92mg/mL)和VC,低于抗氧化剂TBHQ(IC500.375mg/mL)和茶多酚(IC500.382mg/mL);MACP的O-2·清除活性(IC5014.40mg/mL)低于茶多酚(IC504.05mg/mL)和谷胱甘肽(IC500.49mg/mL),却高于TBHQ(IC5017.52mg/mL)。MACP抑制酪氨酸酶的IC50为17.06mg/mL,为常用美白剂——熊果苷的1/70。酶解液经截流相对分子质量分别为10000、4000、1000的超滤膜分离,其活性随相对分子质量的减小而增大,且清除自由基和抑制酪氨酸酶的最大活性相对分子质量都集中在1000以下。对相对分子质量小于1000的MACP成分进行葡聚糖凝胶DEAE-A25阴离子交换分离,得到2个洗脱峰,且活性成分主要集中在第1个峰上。将MACP应用于功能食品或美白化妆品中开发前景良好。
