目的研究高压抗原修复液的p H值对淋巴组织免疫组化染色的影响。方法运用高压的抗原修复方式,按修复液p H值的不同分为p H 6.0枸橼酸抗原修复组和p H8.0EDTA抗原修复组,对76例淋巴组织进行免疫组化染色,每例分别标记抗体CD3、CD20和CD5...目的研究高压抗原修复液的p H值对淋巴组织免疫组化染色的影响。方法运用高压的抗原修复方式,按修复液p H值的不同分为p H 6.0枸橼酸抗原修复组和p H8.0EDTA抗原修复组,对76例淋巴组织进行免疫组化染色,每例分别标记抗体CD3、CD20和CD5。结果 p H 6.0枸橼酸抗原修复组有9例阳性标记不明确,其中5例出现了细胞膜破损,67例符合诊断标准;p H8.0EDTA抗原修复组有60例阳性标记不明确,其中59例出现了细胞膜破损,仅有16例组织染色符合诊断标准,两组间比较差异有统计学意义(P<0.05)。结论免疫组化染色中,使用p H 8.0 EDTA抗原修复液对淋巴组织进行高压修复,致使淋巴细胞胞膜破损,使用p H 6.0枸橼酸高压修复有助于避免这一现象。展开更多
objective To study the clonal evolution of domestic children with lymphoid malignancies inorder to choose the optimal method for the detection of the minimal residual disease. methods To use the PCRwhich employs 22pri...objective To study the clonal evolution of domestic children with lymphoid malignancies inorder to choose the optimal method for the detection of the minimal residual disease. methods To use the PCRwhich employs 22primers and SSCP, investigating the matched samples obtained at diagnosis and at relapse of 13children with lymphoid malignancies. Results The clonal evolution occurred in 54%, 23%, 38%, 46%, 54%, 62%and 54% of 13 cases by PCR employing IgH, TCRγ, TCRVδ2, - Dδ3, TCRPVJ1, TCRPVJ2, TCRPD1,J2, TCRβD2J2primers respectively. NO change occurred only in one of those cases. Clonal evolution at relapse occurred at 50% ofsamples which had only one band PCR product at diagnosis and 78% of those had multiple bands PCR product atdiagnosis. No differences had been detected between diagnosis and relapse by SSCP analyzing those samples inwhich no band change occurred between that at diagnosis and at relapse in PAGE. Conclusion It should beoptimal that the one band rearranged gene at diagnosis acts as main monitoring marker, simultaneously referringto the main band in multiple bands rearranged gene in order to avoid false negative.展开更多
文摘目的研究高压抗原修复液的p H值对淋巴组织免疫组化染色的影响。方法运用高压的抗原修复方式,按修复液p H值的不同分为p H 6.0枸橼酸抗原修复组和p H8.0EDTA抗原修复组,对76例淋巴组织进行免疫组化染色,每例分别标记抗体CD3、CD20和CD5。结果 p H 6.0枸橼酸抗原修复组有9例阳性标记不明确,其中5例出现了细胞膜破损,67例符合诊断标准;p H8.0EDTA抗原修复组有60例阳性标记不明确,其中59例出现了细胞膜破损,仅有16例组织染色符合诊断标准,两组间比较差异有统计学意义(P<0.05)。结论免疫组化染色中,使用p H 8.0 EDTA抗原修复液对淋巴组织进行高压修复,致使淋巴细胞胞膜破损,使用p H 6.0枸橼酸高压修复有助于避免这一现象。
文摘objective To study the clonal evolution of domestic children with lymphoid malignancies inorder to choose the optimal method for the detection of the minimal residual disease. methods To use the PCRwhich employs 22primers and SSCP, investigating the matched samples obtained at diagnosis and at relapse of 13children with lymphoid malignancies. Results The clonal evolution occurred in 54%, 23%, 38%, 46%, 54%, 62%and 54% of 13 cases by PCR employing IgH, TCRγ, TCRVδ2, - Dδ3, TCRPVJ1, TCRPVJ2, TCRPD1,J2, TCRβD2J2primers respectively. NO change occurred only in one of those cases. Clonal evolution at relapse occurred at 50% ofsamples which had only one band PCR product at diagnosis and 78% of those had multiple bands PCR product atdiagnosis. No differences had been detected between diagnosis and relapse by SSCP analyzing those samples inwhich no band change occurred between that at diagnosis and at relapse in PAGE. Conclusion It should beoptimal that the one band rearranged gene at diagnosis acts as main monitoring marker, simultaneously referringto the main band in multiple bands rearranged gene in order to avoid false negative.
