AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time...AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time PCR method,which is capable of differentiation of HBV viral genomic DNA and cccDNA,was used to quantify the total HBV cccDNA.The total HBV viral DNA was quantitated by real-time PCR using a HBV diagnostic kit (PG Biotech,LTD,Shenzhen,China) described previously. RESULTS:For the first time,we measured the level of HBV DNA and cccDNA isolated from ten HBV patients' liver biopsies and sera.In the liver biopsies,cccDNA was detected from all the biopsy samples.The copy number of cccDNA ranged from from 0.03 to 173.1 per cell,the copy number of total HBV DNA ranged from 0.08 to 3 717 per cell.The ratio of total HBV DNA to cccDNA ranged from 1 to 3 406.In the sera, cccDNA was only detected from six samples whereas HBV viral DNA was detected from all ten samples.The ratio of cccDNA to total HBV DNA ranged from 0 to 1.77%.To further investigate the reason why cccDNA could only be detected in some patients' sera,we performed longitudinal studies.The cccDNA was detected from the patients' sera with HBV reactivation but not from the patients' sera without HBV reactivation.The level of cccDNA in the sera was correlated with ALT and viral load in the HBV reactivation patients. CONCLUSION:HBV cccDNA is actively transcribed and replicated in some patients' hepatoo/tes,which is reflected by a high ratio of HBV total DNA vs cccDNA.Detection of cccDNA in the liver biopsy will provide an end-point for the anti-HBV therapy.The occurrence of cccDNA in the sera is an early signal of liver damage,which may be another important clinical parameter.展开更多
In the past 20 years, great progress has been achieved in China in the construction of concrete-filled steel tube （CFST） arch bridges and concrete arch bridges with a CFST skeleton. The span of these bridges has bee...In the past 20 years, great progress has been achieved in China in the construction of concrete-filled steel tube （CFST） arch bridges and concrete arch bridges with a CFST skeleton. The span of these bridges has been increasing rapidly, which is rare in the history of bridge development. The large-scale construction of expressways and high-speed railways demands the development of long-span arch bridges, and advances in design and construction techniques have made it possible to construct such bridges. In the present study, the current status, development, and major innovative technologies of CFST arch bridges and concrete arch bridges with a CFST skeleton in China are elaborated. This paper covers the key con- struction technologies of CFST arch bridges, such as the design, manufacture, and installation of steel tube arch trusses, the preparation and pouring of in-tube concrete, and the construction of the world＇s longest CFST arch bridge-the First Hejiang Yangtze River Bridge. The main construction technologies of rein- forced concrete arch bridges are also presented, which include cable-stayed fastening-hanging cantilever assembly, adjusting the load by means of stay cables, surrounding the concrete for arch rib pouring, and so forth. In addition, the construction of two CFST skeleton concrete arch bridges-the Guangxi Yongning Yong River Bridge and the Yunnan-Guangxi Railway Nanpan River Bridge--is discussed. CFST arch bridges in China have already gained a world-leading position; with the continuous innovation of key technologies, China will become the new leader in promoting the development of arch bridges.展开更多
Primary liver cancer is an important cause of cancer death, and hepatocellular carcinoma （HCC） accounts for 70%-85% of total liver cancer worldwide. Chronic hepatitis B virus （HBV） infection contributes to 〉 75% ...Primary liver cancer is an important cause of cancer death, and hepatocellular carcinoma （HCC） accounts for 70%-85% of total liver cancer worldwide. Chronic hepatitis B virus （HBV） infection contributes to 〉 75% of HCC cases. High serum viral load is the most reliable indicator of viral replication in predicting development of HCC. HBV genotype C is closely associated with HCC in cirrhotic patients aged 〉 50 years, whereas genotype B is associated with development of HCC in non-cirrhotic young patients and postoperative relapse of HCC. Different HBV subgenotypes have distinct patterns of mutations, which are clearly associated with increased risk of HCC. Mutations accumulate during chronic HBV infection and predict occurrence of HCC. Chronic inflammation leads to increased frequency of viral mutation via cellular cytidine deaminase induction. Mutations are negatively selected by host immunity, whereas some immuno-escaped HBV mutants are active in hepatocarcinogenesis. Inflammatory pathways contribute to the inflammation-necrosis-regeneration process, ultimately HCC. Their hallmark molecules can predict malignancy in HBV-infected subjects. Continuing inflammation is involved in hepatocarcinogenesis and closely related to recurrence and metastasis. HBV load, genotype C, viral mutations and expression of inflammatory molecules in HBV-related HCC tissues are significantly associated with poor prognosis. Imbalance between intratumoral CD8^＋T cells and regulatory T cells or Thl and Th2 cytokines in peritumoral tissues can predict prognosis of HBV-related HCC. These factors are important for developing active prevention and surveillance of HBV-infected subjects who are more likely to develop HCC, or for tailoring suitable treatment to improve survival or postpone postoperative recurrence of HCC.展开更多
The Godson project is the first attempt to design high performancegeneral-purpose microprocessors in China. This paper introduces the microarchitecture of theGodson-2 processor which is a 64-bit, 4-issue, out-of-order...The Godson project is the first attempt to design high performancegeneral-purpose microprocessors in China. This paper introduces the microarchitecture of theGodson-2 processor which is a 64-bit, 4-issue, out-of-order execution RISC processor that implementsthe 64-bit MIPS-like instruction set. The adoption of the aggressive out-of-order executiontechniques (such as register mapping, branch prediction, and dynamic scheduling) and cachetechniques (such as non-blocking cache, load speculation, dynamic memory disambiguation) helps theGodson-2 processor to achieve high performance even at not so high frequency. The Godson-2 processorhas been physically implemented on a 6-metal 0.18 μm CMOS technology based on the automaticplacing and routing flow with the help of some crafted library cells and macros. The area of thechip is 6,700 micrometers by 6,200 micrometers and the clock cycle at typical corner is 2.3 ns.展开更多
This paper builds up an accurate nonlinear mathematical model of anelectro-hydraulic force/ torque servo control system, and provides a thorough theoretical analysison the feedforward compensation for extraneous force...This paper builds up an accurate nonlinear mathematical model of anelectro-hydraulic force/ torque servo control system, and provides a thorough theoretical analysison the feedforward compensation for extraneous force/torque, whose limitation is analyzed andrevealed. The nonlinear factors and the servo valve dynamics have much influence on the systemcharacteristics. Subsequently a velocity syn-chronizing-compensation method by using the controlsignal of the control actuator is proposed, which can reduce the lagging effects for the betterperformance. For the reason of similarity between the model of control actuator and that of the loadsimulator, the proposed method performs well against the influence of nonlinear factors. Thesimulations and the experiments confirm that this control scheme results in a quick response,robustness, and excellent ability against disturbance.展开更多
Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, tot...Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.展开更多
Fault diagnosis is vital in manufacturing system.However,the first step of the traditional fault diagnosis method is to process the signal,extract the features and then put the features into a selected classifier for ...Fault diagnosis is vital in manufacturing system.However,the first step of the traditional fault diagnosis method is to process the signal,extract the features and then put the features into a selected classifier for classification.The process of feature extraction depends on the experimenters’experience,and the classification rate of the shallow diagnostic model does not achieve satisfactory results.In view of these problems,this paper proposes a method of converting raw signals into twodimensional images.This method can extract the features of the converted two-dimensional images and eliminate the impact of expert’s experience on the feature extraction process.And it follows by proposing an intelligent diagnosis algorithm based on Convolution Neural Network(CNN),which can automatically accomplish the process of the feature extraction and fault diagnosis.The effect of this method is verified by bearing data.The influence of different sample sizes and different load conditions on the diagnostic capability of this method is analyzed.The results show that the proposed method is effective and can meet the timeliness requirements of fault diagnosis.展开更多
基金SuppoSed by CRCG grant from the University of Hong KongCERG grant from University Grant Council of Hong Kong Research Fund from Science and Technology Commission of Shanghai,China
文摘AIM:To evaluate the covalently closed circle DNA (cccDNA) level of hepatitis B virus (HBV) in patients' liver and sera. METHODS:HBV DNA was isolated from patients' liver biopsies and sera.A sensitive real-time PCR method,which is capable of differentiation of HBV viral genomic DNA and cccDNA,was used to quantify the total HBV cccDNA.The total HBV viral DNA was quantitated by real-time PCR using a HBV diagnostic kit (PG Biotech,LTD,Shenzhen,China) described previously. RESULTS:For the first time,we measured the level of HBV DNA and cccDNA isolated from ten HBV patients' liver biopsies and sera.In the liver biopsies,cccDNA was detected from all the biopsy samples.The copy number of cccDNA ranged from from 0.03 to 173.1 per cell,the copy number of total HBV DNA ranged from 0.08 to 3 717 per cell.The ratio of total HBV DNA to cccDNA ranged from 1 to 3 406.In the sera, cccDNA was only detected from six samples whereas HBV viral DNA was detected from all ten samples.The ratio of cccDNA to total HBV DNA ranged from 0 to 1.77%.To further investigate the reason why cccDNA could only be detected in some patients' sera,we performed longitudinal studies.The cccDNA was detected from the patients' sera with HBV reactivation but not from the patients' sera without HBV reactivation.The level of cccDNA in the sera was correlated with ALT and viral load in the HBV reactivation patients. CONCLUSION:HBV cccDNA is actively transcribed and replicated in some patients' hepatoo/tes,which is reflected by a high ratio of HBV total DNA vs cccDNA.Detection of cccDNA in the liver biopsy will provide an end-point for the anti-HBV therapy.The occurrence of cccDNA in the sera is an early signal of liver damage,which may be another important clinical parameter.
文摘In the past 20 years, great progress has been achieved in China in the construction of concrete-filled steel tube （CFST） arch bridges and concrete arch bridges with a CFST skeleton. The span of these bridges has been increasing rapidly, which is rare in the history of bridge development. The large-scale construction of expressways and high-speed railways demands the development of long-span arch bridges, and advances in design and construction techniques have made it possible to construct such bridges. In the present study, the current status, development, and major innovative technologies of CFST arch bridges and concrete arch bridges with a CFST skeleton in China are elaborated. This paper covers the key con- struction technologies of CFST arch bridges, such as the design, manufacture, and installation of steel tube arch trusses, the preparation and pouring of in-tube concrete, and the construction of the world＇s longest CFST arch bridge-the First Hejiang Yangtze River Bridge. The main construction technologies of rein- forced concrete arch bridges are also presented, which include cable-stayed fastening-hanging cantilever assembly, adjusting the load by means of stay cables, surrounding the concrete for arch rib pouring, and so forth. In addition, the construction of two CFST skeleton concrete arch bridges-the Guangxi Yongning Yong River Bridge and the Yunnan-Guangxi Railway Nanpan River Bridge--is discussed. CFST arch bridges in China have already gained a world-leading position; with the continuous innovation of key technologies, China will become the new leader in promoting the development of arch bridges.
基金Supported by National Natural Science Foundation of China,No. 81025015 and No. 30921006
文摘Primary liver cancer is an important cause of cancer death, and hepatocellular carcinoma （HCC） accounts for 70%-85% of total liver cancer worldwide. Chronic hepatitis B virus （HBV） infection contributes to 〉 75% of HCC cases. High serum viral load is the most reliable indicator of viral replication in predicting development of HCC. HBV genotype C is closely associated with HCC in cirrhotic patients aged 〉 50 years, whereas genotype B is associated with development of HCC in non-cirrhotic young patients and postoperative relapse of HCC. Different HBV subgenotypes have distinct patterns of mutations, which are clearly associated with increased risk of HCC. Mutations accumulate during chronic HBV infection and predict occurrence of HCC. Chronic inflammation leads to increased frequency of viral mutation via cellular cytidine deaminase induction. Mutations are negatively selected by host immunity, whereas some immuno-escaped HBV mutants are active in hepatocarcinogenesis. Inflammatory pathways contribute to the inflammation-necrosis-regeneration process, ultimately HCC. Their hallmark molecules can predict malignancy in HBV-infected subjects. Continuing inflammation is involved in hepatocarcinogenesis and closely related to recurrence and metastasis. HBV load, genotype C, viral mutations and expression of inflammatory molecules in HBV-related HCC tissues are significantly associated with poor prognosis. Imbalance between intratumoral CD8^＋T cells and regulatory T cells or Thl and Th2 cytokines in peritumoral tissues can predict prognosis of HBV-related HCC. These factors are important for developing active prevention and surveillance of HBV-infected subjects who are more likely to develop HCC, or for tailoring suitable treatment to improve survival or postpone postoperative recurrence of HCC.
文摘The Godson project is the first attempt to design high performancegeneral-purpose microprocessors in China. This paper introduces the microarchitecture of theGodson-2 processor which is a 64-bit, 4-issue, out-of-order execution RISC processor that implementsthe 64-bit MIPS-like instruction set. The adoption of the aggressive out-of-order executiontechniques (such as register mapping, branch prediction, and dynamic scheduling) and cachetechniques (such as non-blocking cache, load speculation, dynamic memory disambiguation) helps theGodson-2 processor to achieve high performance even at not so high frequency. The Godson-2 processorhas been physically implemented on a 6-metal 0.18 μm CMOS technology based on the automaticplacing and routing flow with the help of some crafted library cells and macros. The area of thechip is 6,700 micrometers by 6,200 micrometers and the clock cycle at typical corner is 2.3 ns.
文摘This paper builds up an accurate nonlinear mathematical model of anelectro-hydraulic force/ torque servo control system, and provides a thorough theoretical analysison the feedforward compensation for extraneous force/torque, whose limitation is analyzed andrevealed. The nonlinear factors and the servo valve dynamics have much influence on the systemcharacteristics. Subsequently a velocity syn-chronizing-compensation method by using the controlsignal of the control actuator is proposed, which can reduce the lagging effects for the betterperformance. For the reason of similarity between the model of control actuator and that of the loadsimulator, the proposed method performs well against the influence of nonlinear factors. Thesimulations and the experiments confirm that this control scheme results in a quick response,robustness, and excellent ability against disturbance.
基金supported by the High-Tech Research Program (863) of China (No.2009ZX07106-002-004)the National Natural Science Foundation of China (No.30870221,20877093,50808172, 50909091)
文摘Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.
基金co-supported by the National Natural Science Foundation of China(No.51775452)Fundamental Research Funds for the Central Universities,China(Nos.2682019CX35 and 2018GF02)Planning Project of Science&Technology Department of Sichuan Province,China(No.2019YFG0353).
文摘Fault diagnosis is vital in manufacturing system.However,the first step of the traditional fault diagnosis method is to process the signal,extract the features and then put the features into a selected classifier for classification.The process of feature extraction depends on the experimenters’experience,and the classification rate of the shallow diagnostic model does not achieve satisfactory results.In view of these problems,this paper proposes a method of converting raw signals into twodimensional images.This method can extract the features of the converted two-dimensional images and eliminate the impact of expert’s experience on the feature extraction process.And it follows by proposing an intelligent diagnosis algorithm based on Convolution Neural Network(CNN),which can automatically accomplish the process of the feature extraction and fault diagnosis.The effect of this method is verified by bearing data.The influence of different sample sizes and different load conditions on the diagnostic capability of this method is analyzed.The results show that the proposed method is effective and can meet the timeliness requirements of fault diagnosis.
