白血病是目前临床上高发的急危重症,病死率极高,其中白血病干细胞(leukemia stem cells,LSC)发生免疫逃逸是白血病诱导缓解后复发及进展的主要因素。中医药(Traditional Chinese Medicine,TCM)临床诊疗具有鲜明的辨证论治优势,基于“以...白血病是目前临床上高发的急危重症,病死率极高,其中白血病干细胞(leukemia stem cells,LSC)发生免疫逃逸是白血病诱导缓解后复发及进展的主要因素。中医药(Traditional Chinese Medicine,TCM)临床诊疗具有鲜明的辨证论治优势,基于“以平为期”的诊治目的,中医药辨治白血病强调恢复人体机能的“阴阳和谐”,有助于提高机体自身免疫力,符合干预肿瘤细胞免疫逃逸的机制。本文从文献研究及中医理论探讨中医药干预LSC免疫逃逸的机制及其研究进展。展开更多
To assess the value of CD34 + cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34 + cells transfected with F asL or without, pretreated with mitomycin C, was mixed ...To assess the value of CD34 + cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34 + cells transfected with F asL or without, pretreated with mitomycin C, was mixed with leukemic cell line U937 cells in presence or absence of daunorubicin (DNR) or cytosine arabinoside (Ara C). After l8 h, apoptosis of cells was detected by FCM and TUNEL. Induced for l8 h by CD34 + cells transfected with FasL or without, the ratio of apoptos is of U937 cells was (5.0±1.3) %, (10.8±0.6) % ( P < 0.01), respectively. Induced by FasL +CD34 ++DNR, FasL +CD34 ++Ara C, the ratio was (13.4±1.0) % ( P < 0.05), (17.9±1.3)% ( P <0.01), respectively. The result demonstrated that CD34 + cells transfected with exogenous FasL could induce apoptosis of human leukemic cells and showed a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs, suggesting that it was possible to develop a new method in treatment of leukemia.展开更多
Leukemic ceils exist in blood, they are easy to get in touch with target sensitive drugs. Liposomes have been extensively tested as effective, non-toxic drug carriers. Immunoliposomes, which consist of liposomes and h...Leukemic ceils exist in blood, they are easy to get in touch with target sensitive drugs. Liposomes have been extensively tested as effective, non-toxic drug carriers. Immunoliposomes, which consist of liposomes and highly specific antibodies, have been shown to bind specifically to their target cells in vitro and in vivo.In the work presented in this note, we have combined an anti-human T leukemia (?)展开更多
Objective: To explore the expressions of IL-10 on leukemic cells in acute leukemia patients and its significance. Methods: The expressions of IL-10 on leukemic cells in thirty patients was measured by indirect immunof...Objective: To explore the expressions of IL-10 on leukemic cells in acute leukemia patients and its significance. Methods: The expressions of IL-10 on leukemic cells in thirty patients was measured by indirect immunofluorescence technique. Results: Observed yellow-green bright fluorescence on leukemic cells membrane, the positive rate of cells was 10-80%, there were 18 patients expressing IL-10 (18/30, 60%) positively, among them 11 with ANLL (11/19, 58%) and 7 with ALL (7/11, 64%) respectively while that of peripheral mononucleate cells in control group was 13%. Compared with that in the control group, there was a significant increase of positive rate in ANLL and ALL but with no significant difference between ANLL and ALL. Conclusion: IL-10 secreted by leukemic cells, contributed to the immunosuppressive state at the tumor site. This is probably one of the important mechanisms of acute leukemic escape.展开更多
Objective: To establish a HPLC method using fluorometric detection for quantitatively determinating intracellular accumulation of verapamil (VER). Methods: Chromatography column was packed with spherisorb ODS(250&...Objective: To establish a HPLC method using fluorometric detection for quantitatively determinating intracellular accumulation of verapamil (VER). Methods: Chromatography column was packed with spherisorb ODS(250×4.6 mm,10 μm).The mobile phase consisted of the mixture of methanol:NaAC (0.01 mol/L): diethylamine (65:35:0.25). The detect wavelength was 280/310 nm (Ex/Em). Results: The standard curve showed a good correlation between concentration and peak area within the range of 5-50 ng/ml. RSD was 0.86%, and recovery radio of loading sample, 100%. The detection limit for cell sample was 0.2-148 ng/ml. Intracellular accumulation of VER was observed to decrease from a 13 fold to 5 fold in K562/ADM cells, and from a 3.5 fold to 4.3 fold in K562/VER cells and from a 2.1 fold to 6.5 fold in K562/ADM/VER cells, compared with the relevant control cells. Conclusion: HPLC method was proved to be sensitive and specific for using to quantitatively determine the intracellular accumulation of VER.展开更多
文摘白血病是目前临床上高发的急危重症,病死率极高,其中白血病干细胞(leukemia stem cells,LSC)发生免疫逃逸是白血病诱导缓解后复发及进展的主要因素。中医药(Traditional Chinese Medicine,TCM)临床诊疗具有鲜明的辨证论治优势,基于“以平为期”的诊治目的,中医药辨治白血病强调恢复人体机能的“阴阳和谐”,有助于提高机体自身免疫力,符合干预肿瘤细胞免疫逃逸的机制。本文从文献研究及中医理论探讨中医药干预LSC免疫逃逸的机制及其研究进展。
基金the grantof National NatureScience Foundation of China(Serial No. 3 9770 767)
文摘To assess the value of CD34 + cells transferred exogenous Fas ligand (FasL) in inducing apoptosis of human leukemic cells, the CD34 + cells transfected with F asL or without, pretreated with mitomycin C, was mixed with leukemic cell line U937 cells in presence or absence of daunorubicin (DNR) or cytosine arabinoside (Ara C). After l8 h, apoptosis of cells was detected by FCM and TUNEL. Induced for l8 h by CD34 + cells transfected with FasL or without, the ratio of apoptos is of U937 cells was (5.0±1.3) %, (10.8±0.6) % ( P < 0.01), respectively. Induced by FasL +CD34 ++DNR, FasL +CD34 ++Ara C, the ratio was (13.4±1.0) % ( P < 0.05), (17.9±1.3)% ( P <0.01), respectively. The result demonstrated that CD34 + cells transfected with exogenous FasL could induce apoptosis of human leukemic cells and showed a cytotoxic synergistic effect when used in combination with chemotherapeutic drugs, suggesting that it was possible to develop a new method in treatment of leukemia.
文摘Leukemic ceils exist in blood, they are easy to get in touch with target sensitive drugs. Liposomes have been extensively tested as effective, non-toxic drug carriers. Immunoliposomes, which consist of liposomes and highly specific antibodies, have been shown to bind specifically to their target cells in vitro and in vivo.In the work presented in this note, we have combined an anti-human T leukemia (?)
文摘Objective: To explore the expressions of IL-10 on leukemic cells in acute leukemia patients and its significance. Methods: The expressions of IL-10 on leukemic cells in thirty patients was measured by indirect immunofluorescence technique. Results: Observed yellow-green bright fluorescence on leukemic cells membrane, the positive rate of cells was 10-80%, there were 18 patients expressing IL-10 (18/30, 60%) positively, among them 11 with ANLL (11/19, 58%) and 7 with ALL (7/11, 64%) respectively while that of peripheral mononucleate cells in control group was 13%. Compared with that in the control group, there was a significant increase of positive rate in ANLL and ALL but with no significant difference between ANLL and ALL. Conclusion: IL-10 secreted by leukemic cells, contributed to the immunosuppressive state at the tumor site. This is probably one of the important mechanisms of acute leukemic escape.
文摘Objective: To establish a HPLC method using fluorometric detection for quantitatively determinating intracellular accumulation of verapamil (VER). Methods: Chromatography column was packed with spherisorb ODS(250×4.6 mm,10 μm).The mobile phase consisted of the mixture of methanol:NaAC (0.01 mol/L): diethylamine (65:35:0.25). The detect wavelength was 280/310 nm (Ex/Em). Results: The standard curve showed a good correlation between concentration and peak area within the range of 5-50 ng/ml. RSD was 0.86%, and recovery radio of loading sample, 100%. The detection limit for cell sample was 0.2-148 ng/ml. Intracellular accumulation of VER was observed to decrease from a 13 fold to 5 fold in K562/ADM cells, and from a 3.5 fold to 4.3 fold in K562/VER cells and from a 2.1 fold to 6.5 fold in K562/ADM/VER cells, compared with the relevant control cells. Conclusion: HPLC method was proved to be sensitive and specific for using to quantitatively determine the intracellular accumulation of VER.
