用不同浓度配比的生长素和细胞分裂素诱导梨枣叶片和茎段愈伤组织的产生,并研究了不定芽诱导的最佳配方,建立了梨枣叶片和茎段的再生体系。结果表明,梨枣叶片愈伤组织诱导的最佳培养基为M S+2,4-D 1.5m g.L-1+6-BA 0.5 m g.L-1;茎段为M ...用不同浓度配比的生长素和细胞分裂素诱导梨枣叶片和茎段愈伤组织的产生,并研究了不定芽诱导的最佳配方,建立了梨枣叶片和茎段的再生体系。结果表明,梨枣叶片愈伤组织诱导的最佳培养基为M S+2,4-D 1.5m g.L-1+6-BA 0.5 m g.L-1;茎段为M S+2,4-D 1.0 m g.L-1+6-BA 0.5 m g.L-1。叶片不定芽诱导的最佳培养基为M S+IBA 0.1 m g.L-1+6-BA 1.5 m g.L-1。A gNO3能阻止叶片外植体褐化并有效地促进叶片愈伤组织分化。茎段能在同一培养基上产生愈伤组织并直接分化出不定芽。展开更多
Wheat along with rice and maize is fulfilling half of the calories demands of the world. Global Wheat production has increased tremendously since green revolution in 1960’s and helped in minimizing hunger and malnutr...Wheat along with rice and maize is fulfilling half of the calories demands of the world. Global Wheat production has increased tremendously since green revolution in 1960’s and helped in minimizing hunger and malnutrition. Developing countries, which consume 60% of the global wheat production, have shown a higher yield increase than the developed countries in the past [1]. It was driven by the hunger prevalence in these countries and was attributable to the introduction of high yielding and rusted resistant semi dwarf varieties developed under the collaborative efforts of International and National research systems during the last 50 years. Whereas, climate change and the emergence of new pests and diseases are threatening the food sustainability. The evolution of new races of disease pathogens like stem rust (Ug 99) is of serious concern. In order to feed the ever increasing population we have to increase wheat production at the rate 1.6% which can be achieved by developing high yielding varieties having a good tolerance level for biotic and abiotic stresses.展开更多
文摘用不同浓度配比的生长素和细胞分裂素诱导梨枣叶片和茎段愈伤组织的产生,并研究了不定芽诱导的最佳配方,建立了梨枣叶片和茎段的再生体系。结果表明,梨枣叶片愈伤组织诱导的最佳培养基为M S+2,4-D 1.5m g.L-1+6-BA 0.5 m g.L-1;茎段为M S+2,4-D 1.0 m g.L-1+6-BA 0.5 m g.L-1。叶片不定芽诱导的最佳培养基为M S+IBA 0.1 m g.L-1+6-BA 1.5 m g.L-1。A gNO3能阻止叶片外植体褐化并有效地促进叶片愈伤组织分化。茎段能在同一培养基上产生愈伤组织并直接分化出不定芽。
文摘Wheat along with rice and maize is fulfilling half of the calories demands of the world. Global Wheat production has increased tremendously since green revolution in 1960’s and helped in minimizing hunger and malnutrition. Developing countries, which consume 60% of the global wheat production, have shown a higher yield increase than the developed countries in the past [1]. It was driven by the hunger prevalence in these countries and was attributable to the introduction of high yielding and rusted resistant semi dwarf varieties developed under the collaborative efforts of International and National research systems during the last 50 years. Whereas, climate change and the emergence of new pests and diseases are threatening the food sustainability. The evolution of new races of disease pathogens like stem rust (Ug 99) is of serious concern. In order to feed the ever increasing population we have to increase wheat production at the rate 1.6% which can be achieved by developing high yielding varieties having a good tolerance level for biotic and abiotic stresses.
