In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet （UV） mutation and self-tolerant mutation of Bacillus lateros...In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet （UV） mutation and self-tolerant mutation of Bacillus laterosporus A7. A high-yield strain A-94-7 was obtained and the spergualin productivity was 4. 1-fold of the parent strain. The genetic stability of the high-yield strain Bacillus laterosporus A-94-7 was very good. Aider fermentation medium optimization, Bacillus laterosporus A-94-7 was able to produce 380 mg· L^-1 The spergualin productivity in Bacillus laterosporus A7 was increased 4.75-fold by mutation and medium optimization.展开更多
[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through op...[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through optimizing the medium of Brevibacil us laterosporus. [Method] A total of 10 factors were tested for their function of improving antibiotics yield of BL-21 by single-factor test. And the indicative bacterium was screened. [Result] The best indicative bacterium was Al-ternaria solani, the optimum process parameters in shaking flask on fermentation consisted of initial pH of 8.0, culture at 30 ℃, loadage 90 ml/250 ml, rotation 180 r/min, and fermentation time of 48 h. The results also indicated that glucose was the best carbon source for the antibiotics productivity of BL-21, and beef extract was the best nitrogen source. In addition, univalent ion Cl＋ and divalent ion Mn2＋ stimu-lated BL-21 antibacterial active component biosynthesis. [Conclusion] Optimized medi-um is more conducive to produce antimicrobial substances.展开更多
基金Supported by Program for Innovative Research Team of Northeast Agricultural University
文摘In order to screen a high-yield spergualin-production strain and an optimal fermentation medium, large numbers of isolates were selected after ultraviolet （UV） mutation and self-tolerant mutation of Bacillus laterosporus A7. A high-yield strain A-94-7 was obtained and the spergualin productivity was 4. 1-fold of the parent strain. The genetic stability of the high-yield strain Bacillus laterosporus A-94-7 was very good. Aider fermentation medium optimization, Bacillus laterosporus A-94-7 was able to produce 380 mg· L^-1 The spergualin productivity in Bacillus laterosporus A7 was increased 4.75-fold by mutation and medium optimization.
基金Supported by National Natural Science Foundation of China(31370511)Natural Science Foundation of Heilongjiang Province(C201203)Open Fund of Key Laboratory of Microbiology of Heilongjiang University(2012MOI-1)~~
文摘[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through optimizing the medium of Brevibacil us laterosporus. [Method] A total of 10 factors were tested for their function of improving antibiotics yield of BL-21 by single-factor test. And the indicative bacterium was screened. [Result] The best indicative bacterium was Al-ternaria solani, the optimum process parameters in shaking flask on fermentation consisted of initial pH of 8.0, culture at 30 ℃, loadage 90 ml/250 ml, rotation 180 r/min, and fermentation time of 48 h. The results also indicated that glucose was the best carbon source for the antibiotics productivity of BL-21, and beef extract was the best nitrogen source. In addition, univalent ion Cl＋ and divalent ion Mn2＋ stimu-lated BL-21 antibacterial active component biosynthesis. [Conclusion] Optimized medi-um is more conducive to produce antimicrobial substances.
