目的:通过NCBI(National Center for Biotechnology Information)数据库筛选可以应用于洗涤工业的蛋白酶,使用地衣芽孢杆菌进行高效表达。方法:利用同源重组的方式将蛋白酶基因整合到地衣芽孢杆菌基因组中,研究重组蛋白酶的酶学特性和...目的:通过NCBI(National Center for Biotechnology Information)数据库筛选可以应用于洗涤工业的蛋白酶,使用地衣芽孢杆菌进行高效表达。方法:利用同源重组的方式将蛋白酶基因整合到地衣芽孢杆菌基因组中,研究重组蛋白酶的酶学特性和洗涤效果。结果:该重组酶的最适作用温度为60℃,最适pH为11.0;AH-101具有良好的耐热性,可以在20~55℃范围内保持稳定,在60℃保温1 h,剩余活性仍高于30%;此外,该酶与表面活性剂和液体洗涤剂具有良好的兼容性,在终浓度为0.1%和0.5%的表面活性剂和液体洗涤剂中孵育2 h,重组酶的酶活力损失小于30%。结论:成功构建了一株含有外源蛋白酶的地衣芽孢杆菌重组菌株,对重组蛋白酶的特性和洗涤效果进行研究,该重组蛋白酶可以作为环保型酶制剂应用到洗涤工业中。展开更多
在以设计模型为核心的信息传递模式下,根据工艺设计过程的信息定义、信息传递和信息管理要求归纳出工艺模型基本属性,包括工艺过程信息定义、工艺设计意图表达和工艺信息数据载体3个方面。以参数化为主要方式规范地定义了整个工艺设计...在以设计模型为核心的信息传递模式下,根据工艺设计过程的信息定义、信息传递和信息管理要求归纳出工艺模型基本属性,包括工艺过程信息定义、工艺设计意图表达和工艺信息数据载体3个方面。以参数化为主要方式规范地定义了整个工艺设计过程中工艺设计依据、工艺规划、详细工艺设计、工艺文件导出和工艺审批与管理等环节相关数据。指出CAD系统中可应用于工艺模型建模的5种关联机制,并根据工艺模型集成化特点设计出File Link和Capture/Resource Link两种新的关联机制。在基于模型定义(Model Based Definition,MBD)技术的参数化与三维标注表达方式基础上设计了几何元素引用、捕获视图关联与文件关联表达方法,丰富了MBD技术的表达方式。最后给出了工艺模型在三维CAPP系统集成环境下的设计流程与建模实例。展开更多
To improve aerodynamic performance of wind turbine airfoils,the shape profile characteristic of the airfoil is investigated.Application of conformal transformation,one functional and integrated expression of wind turb...To improve aerodynamic performance of wind turbine airfoils,the shape profile characteristic of the airfoil is investigated.Application of conformal transformation,one functional and integrated expression of wind turbine airfoils is presented.Using the boundary layer theory,the aerodynamic model with roughness of wind turbine airfoils is introduced by studying flow separation around the airfoil.Based on the shape expression and aerodynamic performance of airfoils,the function design of wind turbine airfoils is carried out that the maximum lift-drag ratio and low roughness sensitivity are designed objects.Three wind turbines airfoils with different thickness are gained which are used at tip part of blades.As an example,the aerodynamic performance of one designed airfoil with relative thickness of 15%is simulated in different conditions of clean surface,rough surface,laminar flow and turbulent flow.The comparison of aerodynamic performance between the designed airfoil and one popular NACA airfoil is completed which can verify the better performance of the designed airfoil and reliability of the designed method.展开更多
文摘目的:通过NCBI(National Center for Biotechnology Information)数据库筛选可以应用于洗涤工业的蛋白酶,使用地衣芽孢杆菌进行高效表达。方法:利用同源重组的方式将蛋白酶基因整合到地衣芽孢杆菌基因组中,研究重组蛋白酶的酶学特性和洗涤效果。结果:该重组酶的最适作用温度为60℃,最适pH为11.0;AH-101具有良好的耐热性,可以在20~55℃范围内保持稳定,在60℃保温1 h,剩余活性仍高于30%;此外,该酶与表面活性剂和液体洗涤剂具有良好的兼容性,在终浓度为0.1%和0.5%的表面活性剂和液体洗涤剂中孵育2 h,重组酶的酶活力损失小于30%。结论:成功构建了一株含有外源蛋白酶的地衣芽孢杆菌重组菌株,对重组蛋白酶的特性和洗涤效果进行研究,该重组蛋白酶可以作为环保型酶制剂应用到洗涤工业中。
文摘在以设计模型为核心的信息传递模式下,根据工艺设计过程的信息定义、信息传递和信息管理要求归纳出工艺模型基本属性,包括工艺过程信息定义、工艺设计意图表达和工艺信息数据载体3个方面。以参数化为主要方式规范地定义了整个工艺设计过程中工艺设计依据、工艺规划、详细工艺设计、工艺文件导出和工艺审批与管理等环节相关数据。指出CAD系统中可应用于工艺模型建模的5种关联机制,并根据工艺模型集成化特点设计出File Link和Capture/Resource Link两种新的关联机制。在基于模型定义(Model Based Definition,MBD)技术的参数化与三维标注表达方式基础上设计了几何元素引用、捕获视图关联与文件关联表达方法,丰富了MBD技术的表达方式。最后给出了工艺模型在三维CAPP系统集成环境下的设计流程与建模实例。
基金Supported by the National Natural Science Foundation of China(No.51205430)Natural Science Foundation of ChongQing(No.cstc2011ijA70002)China Postdoctoral Science Foundation(No.2013T60842)
文摘To improve aerodynamic performance of wind turbine airfoils,the shape profile characteristic of the airfoil is investigated.Application of conformal transformation,one functional and integrated expression of wind turbine airfoils is presented.Using the boundary layer theory,the aerodynamic model with roughness of wind turbine airfoils is introduced by studying flow separation around the airfoil.Based on the shape expression and aerodynamic performance of airfoils,the function design of wind turbine airfoils is carried out that the maximum lift-drag ratio and low roughness sensitivity are designed objects.Three wind turbines airfoils with different thickness are gained which are used at tip part of blades.As an example,the aerodynamic performance of one designed airfoil with relative thickness of 15%is simulated in different conditions of clean surface,rough surface,laminar flow and turbulent flow.The comparison of aerodynamic performance between the designed airfoil and one popular NACA airfoil is completed which can verify the better performance of the designed airfoil and reliability of the designed method.
