AIM: To clarify whether histone deacetylase inhibitors histone deacetylase inhibitors (HDACIs) can sensitize hepatocellular carcinoma (HCC) cells to sorafenib treatment. METHODS: Bax, Bcl-2, ATG5-ATG12, p21, and p27 p...AIM: To clarify whether histone deacetylase inhibitors histone deacetylase inhibitors (HDACIs) can sensitize hepatocellular carcinoma (HCC) cells to sorafenib treatment. METHODS: Bax, Bcl-2, ATG5-ATG12, p21, and p27 protein levels in Hep3B, HepG2, and PLC/PRF/5 cells were examined by Western blot. CCK8 and a fluoro-metric caspase-3 assay were used to examine cellular viability and apoptosis levels. The effect of Beclin-1 on sensitization of HCC cells to sorafenib was examined by transfecting Beclin-1 siRNA into Hep3B, HepG2, and PLC/PRF/5 cells. RESULTS: Autophagy inhibition enhances the inhibitory effects of vorinostat and sorafenib alone or in combination on HCC cell growth. Vorinostat and sorafenib synergistically induced apoptosis and cell cycle alterations. Western blot data indicated that HDACIs and Beclin- 1 knockdown increased the p53 acetylation level. The knockdown of Beclin-1 enhanced the synergistic effect of the combination of vorinostat with sorafenib. CONCLUSION: HDACIs can sensitize HCC cells to sorafenib treatment by regulating the acetylation level of Beclin-1. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved.展开更多
In energy eonservatisn, release and transduction of ATPase are of vital importance. A microcalorimetric method was used to study the inhibitory effect of halogen compounds, e.g. ICH2COONa, BrCH2COONa, ICH2CONH2, on th...In energy eonservatisn, release and transduction of ATPase are of vital importance. A microcalorimetric method was used to study the inhibitory effect of halogen compounds, e.g. ICH2COONa, BrCH2COONa, ICH2CONH2, on the activity of Mg2+ dependent soluble ATPase （F1） from pig heart mitochondria. It was found that the degree展开更多
BACKGROUND： Scorpion venom from Buthus martensii Karsch is a kind of protein toxin secreted by scorpion tail. According to records of Pen-ts ＇ao Kan-mu, the dried scorpion＇s body and tail are used as medicine, whic...BACKGROUND： Scorpion venom from Buthus martensii Karsch is a kind of protein toxin secreted by scorpion tail. According to records of Pen-ts ＇ao Kan-mu, the dried scorpion＇s body and tail are used as medicine, which mainly treating rheumatism numbness, pain and convulsion. Some researches consider that scorpion toxin is the main pharmacological active component of the dried scorpion, and it has 4 times stronger analgesic effect on somatic pain than morphine. OBJECTIVE： To observe the interventional effect of scorpion venom of Buthus martensii Karsch analgesic active peptide on somatic pain. DESIGN： A randomized and controlled trial SETTING： Department of Physiology, Shenyang Medical College MATERIALS： Totally 80 Wistar rats （provided by Animal Center of Shenyang Medical College）, male and female in half, aged 3 to 4 months, weighed 250 to 350 g, were used in this trial. They were randomly divided into 4 groups： experimental group, control group, morphine group and naloxone group, with 20 in each group. Analgesia active peptide from buthus martensii karsch venom （provided by Biochemical Department of Shenyang Pharmaceutical University, 1 mL/dosage）, morphine （produced by Shanghai First Pharmaceutical Manufacturing Plant）, naloxone （opium acceptor inhibitor, American Sigma Corporation）, AT- AC-350 data-processing machine （Nihon Kohden Corporation）, X-Y recording instrument （Nihon Kohden Corporation）. METHODS： The experiment was conducted in the Department of Physiology, Shenyang Medical College from July 2003 to July 2005. ① After all the animals were anesthetized, common peroneal nerve is dissected and ligated at middle part. Posterior nucleus group of thalamus （PO） and the tail nuclear were oriented according to G. Paxino rat brain three-dimensional orientation atlas, Glass micro electrode was inserted into PO as guiding electrode, connected with ATAC-350 data-processing machine and X-Y recording instrument, to record the unit evoked potential of PO, taking the evok展开更多
When the cellulase hydrolyses carboxymethyl-cellulose-Na(CMC-Na), it is not fit to formula, V=KE, between reaction velocity and concentration of enzyme. Therefore, a factor that inhibits this enzyme reaction, which is...When the cellulase hydrolyses carboxymethyl-cellulose-Na(CMC-Na), it is not fit to formula, V=KE, between reaction velocity and concentration of enzyme. Therefore, a factor that inhibits this enzyme reaction, which is called the cellulase endo-inhibitor, was discovered in the fermentative medium of Trichoderma spp. It was shown as a single band through electrophoresis, indicating it is homogeneous and the inhibitor展开更多
The clarification of the mechanism about callus formation is a basic work for plant tissure and cell culture. Previously, there have been some researches on the changes of nuclear acids, proteins, isoenzymes and respi...The clarification of the mechanism about callus formation is a basic work for plant tissure and cell culture. Previously, there have been some researches on the changes of nuclear acids, proteins, isoenzymes and respiration metabolism in this aspect. The major inducer展开更多
基金Supported by Eastern Hepatobiliary Surgery Hospital of the Second Military Medical University
文摘AIM: To clarify whether histone deacetylase inhibitors histone deacetylase inhibitors (HDACIs) can sensitize hepatocellular carcinoma (HCC) cells to sorafenib treatment. METHODS: Bax, Bcl-2, ATG5-ATG12, p21, and p27 protein levels in Hep3B, HepG2, and PLC/PRF/5 cells were examined by Western blot. CCK8 and a fluoro-metric caspase-3 assay were used to examine cellular viability and apoptosis levels. The effect of Beclin-1 on sensitization of HCC cells to sorafenib was examined by transfecting Beclin-1 siRNA into Hep3B, HepG2, and PLC/PRF/5 cells. RESULTS: Autophagy inhibition enhances the inhibitory effects of vorinostat and sorafenib alone or in combination on HCC cell growth. Vorinostat and sorafenib synergistically induced apoptosis and cell cycle alterations. Western blot data indicated that HDACIs and Beclin- 1 knockdown increased the p53 acetylation level. The knockdown of Beclin-1 enhanced the synergistic effect of the combination of vorinostat with sorafenib. CONCLUSION: HDACIs can sensitize HCC cells to sorafenib treatment by regulating the acetylation level of Beclin-1. (C) 2014 Baishideng Publishing Group Co., Limited. All rights reserved.
文摘In energy eonservatisn, release and transduction of ATPase are of vital importance. A microcalorimetric method was used to study the inhibitory effect of halogen compounds, e.g. ICH2COONa, BrCH2COONa, ICH2CONH2, on the activity of Mg2+ dependent soluble ATPase （F1） from pig heart mitochondria. It was found that the degree
文摘BACKGROUND： Scorpion venom from Buthus martensii Karsch is a kind of protein toxin secreted by scorpion tail. According to records of Pen-ts ＇ao Kan-mu, the dried scorpion＇s body and tail are used as medicine, which mainly treating rheumatism numbness, pain and convulsion. Some researches consider that scorpion toxin is the main pharmacological active component of the dried scorpion, and it has 4 times stronger analgesic effect on somatic pain than morphine. OBJECTIVE： To observe the interventional effect of scorpion venom of Buthus martensii Karsch analgesic active peptide on somatic pain. DESIGN： A randomized and controlled trial SETTING： Department of Physiology, Shenyang Medical College MATERIALS： Totally 80 Wistar rats （provided by Animal Center of Shenyang Medical College）, male and female in half, aged 3 to 4 months, weighed 250 to 350 g, were used in this trial. They were randomly divided into 4 groups： experimental group, control group, morphine group and naloxone group, with 20 in each group. Analgesia active peptide from buthus martensii karsch venom （provided by Biochemical Department of Shenyang Pharmaceutical University, 1 mL/dosage）, morphine （produced by Shanghai First Pharmaceutical Manufacturing Plant）, naloxone （opium acceptor inhibitor, American Sigma Corporation）, AT- AC-350 data-processing machine （Nihon Kohden Corporation）, X-Y recording instrument （Nihon Kohden Corporation）. METHODS： The experiment was conducted in the Department of Physiology, Shenyang Medical College from July 2003 to July 2005. ① After all the animals were anesthetized, common peroneal nerve is dissected and ligated at middle part. Posterior nucleus group of thalamus （PO） and the tail nuclear were oriented according to G. Paxino rat brain three-dimensional orientation atlas, Glass micro electrode was inserted into PO as guiding electrode, connected with ATAC-350 data-processing machine and X-Y recording instrument, to record the unit evoked potential of PO, taking the evok
文摘When the cellulase hydrolyses carboxymethyl-cellulose-Na(CMC-Na), it is not fit to formula, V=KE, between reaction velocity and concentration of enzyme. Therefore, a factor that inhibits this enzyme reaction, which is called the cellulase endo-inhibitor, was discovered in the fermentative medium of Trichoderma spp. It was shown as a single band through electrophoresis, indicating it is homogeneous and the inhibitor
基金Project supported by the National Natural Science Foundation of China.
文摘The clarification of the mechanism about callus formation is a basic work for plant tissure and cell culture. Previously, there have been some researches on the changes of nuclear acids, proteins, isoenzymes and respiration metabolism in this aspect. The major inducer
