为探讨银翘解毒软胶囊对甲型H1N1流感病毒PR8株感染小鼠肺炎模型的治疗作用机制,在滴鼻感染正常小鼠建立流感病毒肺炎模型的基础上,观察小鼠肺指数、病死率,并进一步进行分子机制研究。分别采用实时荧光定量PCR(real time RT-PCR)及West...为探讨银翘解毒软胶囊对甲型H1N1流感病毒PR8株感染小鼠肺炎模型的治疗作用机制,在滴鼻感染正常小鼠建立流感病毒肺炎模型的基础上,观察小鼠肺指数、病死率,并进一步进行分子机制研究。分别采用实时荧光定量PCR(real time RT-PCR)及Western blotting技术,在流感病毒感染后1、3、5、7天,检测小鼠肺组织中病毒载量的变化以及肺组织中M1蛋白相对含量的变化。结果显示,银翘解毒软胶囊(1和0.5 g.kg-1)在感染后第3、5、7天可明显降低正常小鼠感染后肺指数,并且两剂量组动物的死亡数显著降低,存活天数显著增加;银翘解毒软胶囊可以降低肺组织病毒载量,其中1 g.kg-1剂量组在各时间点均可降低其表达量(P<0.05,P<0.01),0.5 g.kg-1剂量组在第3、5、7天均可降低病毒载量(P<0.05,P<0.01);银翘解毒软胶囊可以降低肺组织M1蛋白相对表达量,其中1和0.5 g.kg-1剂量组在感染后第3、7天,与模型对照组相比明显低表达(P<0.05,P<0.01)。结果表明,银翘解毒软胶囊对甲型流感病毒有良好的治疗作用,并可能通过下调小鼠肺组织内病毒载量及M1蛋白相对表达量,来发挥其抗病毒作用。展开更多
Viral isolation in cell cultures has been regarded for decades as the“gold standard”for the laboratory diagnosis of influenza viral infections.Not all viral strains could be isolated from clinical samples.This study...Viral isolation in cell cultures has been regarded for decades as the“gold standard”for the laboratory diagnosis of influenza viral infections.Not all viral strains could be isolated from clinical samples.This study aimed to quantify the viral load in the samples before isolation to save working time and improve working efficiency.Four hundred samples from patients with influenza-like cases were confirmed pdmH1N1 positive(200 cases)and B Victoria(BV)positive(200 cases)by whole-genome sequencing and analyzed by ddPCR for viral load in samples before isolation,and isolation results were verified by hemagglutination(HA)assay and hemagglutination-inhibition(HI)tests.Probit regression analysis was used to calculate the isolation viral load limit with a 95%probability level by SPSS 19.0 software.The results showed that the isolation limit of viral load was 4.9×10^(4)(95%CI:2.5×10^(4)–9.0×10^(4))copies/mL for pdmH1N1 and 1.9×10^(4)(95%CI:7.8×103–3.6×10^(4))copies/mL for BV.The isolation rate of clinical samples is positively correlated with the viral load in clinical samples,which can be used for viral culture,providing important guidance for daily work.展开更多
基金Capital Health Development and Research of Special(2022‐1G‐3014)Beijing Science and Technology Planning Project of Beijing Science and Technology Commission(Z211100002521015 and Z211100002521019)+1 种基金National Key R&D Program of China(2021ZD0114100,2021ZD0114103)Cooperation Research Project(2020‐jk‐cd‐026).
文摘Viral isolation in cell cultures has been regarded for decades as the“gold standard”for the laboratory diagnosis of influenza viral infections.Not all viral strains could be isolated from clinical samples.This study aimed to quantify the viral load in the samples before isolation to save working time and improve working efficiency.Four hundred samples from patients with influenza-like cases were confirmed pdmH1N1 positive(200 cases)and B Victoria(BV)positive(200 cases)by whole-genome sequencing and analyzed by ddPCR for viral load in samples before isolation,and isolation results were verified by hemagglutination(HA)assay and hemagglutination-inhibition(HI)tests.Probit regression analysis was used to calculate the isolation viral load limit with a 95%probability level by SPSS 19.0 software.The results showed that the isolation limit of viral load was 4.9×10^(4)(95%CI:2.5×10^(4)–9.0×10^(4))copies/mL for pdmH1N1 and 1.9×10^(4)(95%CI:7.8×103–3.6×10^(4))copies/mL for BV.The isolation rate of clinical samples is positively correlated with the viral load in clinical samples,which can be used for viral culture,providing important guidance for daily work.
