A high performance liquid chromatography-ultraviolet(HPLC-UV)fingerprint method for the overall chemical analysis of edible mushrooms was established based on Auricularia heimuer for the first time,and then applied to...A high performance liquid chromatography-ultraviolet(HPLC-UV)fingerprint method for the overall chemical analysis of edible mushrooms was established based on Auricularia heimuer for the first time,and then applied to analyze 60 batches of A.heimuer,Auricularia cornea.Auricularia cornea*Yu Muer’and TremeUa fuciformis.A total of 9 characteristic peaks of A.heimuer.11 characteristic peaks of A.cornea,6 characteristic peaks of A.cornea‘Yu Muer’,and 9 characteristic peaks of I fuciformis were designated.Then,a combinatory analysis,including similarity evaluation,hierarchical cluster analysis and principal component analysis,revealed the chemical consistency and difference between samples from the same and different species.The HPLC fingerprint method established in this paper could be used to characterize the components of A.heimuer,A.cornea,A.cornea‘Yu Muer’,and T.fuciformis and discriminate the 4 edible mushrooms effectively in combination with pattern recognition analysis.展开更多
Auricularia heimuer,an edible jelly fungus,is in considerable demand in Asia due to its high nutritive,economic and medicinal values.RNA-Seq was used to investigate and analyze the mycelium transcriptome of A.heimuer ...Auricularia heimuer,an edible jelly fungus,is in considerable demand in Asia due to its high nutritive,economic and medicinal values.RNA-Seq was used to investigate and analyze the mycelium transcriptome of A.heimuer for gene discovery.A total of 26,857 unigenes with an N50 length of 1333 bp were assembled by de novo sequencing.In addition,unigenes were annotated by publicly available databases,including gene descriptions,gene ontology(GO),clusters of orthologous group(COG),Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,and protein family(Pfam)terms.A.heimuer was also studied for its wood degradation ability.Thirty-eight putative FOLymes(fungal oxidative lignin enzymes)and 251 CAZymes(carbohydrate-active enzymes)were located from A.heimuer transcriptome.Our study provides a comprehensive sequence resource for A.heimuer at the transcriptional level,which will lay a strong foundation for functional genomics studies and gene discovery of this promising fungus.展开更多
基金This publication was financially supported by the National Key R&D Program of China(2018YFD0400202 and 2018YFD0400200).
文摘A high performance liquid chromatography-ultraviolet(HPLC-UV)fingerprint method for the overall chemical analysis of edible mushrooms was established based on Auricularia heimuer for the first time,and then applied to analyze 60 batches of A.heimuer,Auricularia cornea.Auricularia cornea*Yu Muer’and TremeUa fuciformis.A total of 9 characteristic peaks of A.heimuer.11 characteristic peaks of A.cornea,6 characteristic peaks of A.cornea‘Yu Muer’,and 9 characteristic peaks of I fuciformis were designated.Then,a combinatory analysis,including similarity evaluation,hierarchical cluster analysis and principal component analysis,revealed the chemical consistency and difference between samples from the same and different species.The HPLC fingerprint method established in this paper could be used to characterize the components of A.heimuer,A.cornea,A.cornea‘Yu Muer’,and T.fuciformis and discriminate the 4 edible mushrooms effectively in combination with pattern recognition analysis.
基金The work was supported by the Fundamental Research Funds for the Central Universities,(Project No.2572017CF01)the Harbin University Scientific Research Foundation project,(Project No.HUDF2018105).
文摘Auricularia heimuer,an edible jelly fungus,is in considerable demand in Asia due to its high nutritive,economic and medicinal values.RNA-Seq was used to investigate and analyze the mycelium transcriptome of A.heimuer for gene discovery.A total of 26,857 unigenes with an N50 length of 1333 bp were assembled by de novo sequencing.In addition,unigenes were annotated by publicly available databases,including gene descriptions,gene ontology(GO),clusters of orthologous group(COG),Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,and protein family(Pfam)terms.A.heimuer was also studied for its wood degradation ability.Thirty-eight putative FOLymes(fungal oxidative lignin enzymes)and 251 CAZymes(carbohydrate-active enzymes)were located from A.heimuer transcriptome.Our study provides a comprehensive sequence resource for A.heimuer at the transcriptional level,which will lay a strong foundation for functional genomics studies and gene discovery of this promising fungus.
