摘要
黑色素是一种广泛存在于动物、植物、细菌及真菌中的生物色素,具有多种生物功能及良好的生物活性。黑木耳以"黑"出名,其富含的黑色素具有广阔开发应用价值。本研究旨在评价黑木耳黑色素对急性肝损伤的改善作用。首先应用傅里叶红外光谱初步对提取的黑木耳黑色素进行鉴定,再通过DPPH自由基及羟基自由基清除实验证实提取的黑木耳黑色素体外抗氧化能力,并进一步以四氯化碳致小鼠急性肝损伤为模型,通过检测血清酶指标、肝功指标的变化及病理切片情况,来评价黑木耳黑色素体内抗氧化及保肝效果。结果表明,提取的黑木耳黑色素具有黑色素特征的官能团结构和良好的体外抗氧化能力,对DPPH自由基和羟基(OH)自由基清除的EC50分别为0.0887mg/mL、2.2030mg/mL;动物体内实验中,与模型组对比,给药组(黑木耳黑色素)的小鼠血清中ALT、AST含量显著降低(P<0.01),肝脏中MDA含量显著降低(P<0.01)和SOD活性显著升高(P<0.01),并且肝细胞病理状态明显改善。本文报道了黑木耳黑色素在体内能有效改善四氯化碳诱导的小鼠肝损伤,为黑木耳的功能产品开发提供了新思路和研究基础。
Melanin is one of biological pigment widely existing in animals, plants, bacteria and fungi, with broadly biological functions and activity. Auricularia heimuer is known as "black", and its melanin component is worthy of development and application. This study aims at evaluation of the improvement effects of Auricularia heimuer melanin on acute liver injuried mice. Extraction of Auricularia heimuer was analysed by fourier transform infrared spectrometer and its antioxidant activity in vitro was verified by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and hydroxyl radical. CCl4-induced acute liver injury model was established, and the antioxidant in vivo and liver improvement effects of Auricularia heimuer melanin were assessed by determining the change of serum enzyme, liver function index and the histological examination of the liver tissue.The result showed that extraction of Auricularia heimuer had the same functional groups and antioxidant activity in vitro as melanin reported in literature, with the EC50 values of scavenging DPPH and OH radical of 0.0887 mg/mL and 2.2030 mg/mL respectively. In vivo, Auricularia heimuer melanin had significantly reduced levels of ALT and AST in serum and preserved activities of SOD, inhibited MDA in liver, and improved the damages of the liver tissue. The study discovered that Auricularia heimuer melanin had efficiently improved CCl4-induced acute liver injury in vivo, providing a new direction for exploration of Auricularia heimuer melanin functional product.
作者
刘城移
戚梦
吕虹燕
林文雄
吴小平
傅俊生
LIU Cheng‐Yi;QI Meng;LV Hong‐Yan;LIN Wen‐Xiong;WU Xiao‐Ping;FU Jun‐Sheng(College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)
出处
《菌物学报》
CAS
CSCD
北大核心
2018年第12期1741-1750,共10页
Mycosystema
基金
国家重点研发计划资助(2018YFD0400200)
国家自然科学基金(81503187)
福建农林大学现代种业工程研究院建设经费~~
关键词
黑木耳黑色素
肝损伤
抗氧化
保肝作用
Auricularia heimuer melanin
liver injury
antioxidant
liver‐protection
