目的:通过气管内给药的方法比较不同浓度人脐带间充质干细胞气管内移植对重度烧伤致急性肺损伤大鼠的保护作用。方法:建立50%面积全层烫伤大鼠模型,将75只成熟雄性Wistar大鼠随机分为正常对照组(A组)、生理盐水组(B组)、1×10~5HUCM...目的:通过气管内给药的方法比较不同浓度人脐带间充质干细胞气管内移植对重度烧伤致急性肺损伤大鼠的保护作用。方法:建立50%面积全层烫伤大鼠模型,将75只成熟雄性Wistar大鼠随机分为正常对照组(A组)、生理盐水组(B组)、1×10~5HUCMSCs移植组(C)、5×10~5HUCMSCs移植组(D)、1×10~6HUCMSCs移植组(E),每组15只,B组及移植组(C、D、E组)烫伤后立即液体复苏,B组烫伤后气管内滴注0.2 m L生理盐水,移植组气管内滴注不同浓度h UCMSCs,分别在移植后的天第1、3、7天留取大鼠肺组织标本,HE染色观察肺组织病理变化,MPO、CD68免疫组化染色观察肺组织中性粒细胞及肺巨噬细胞阳性表达情况。结果:肺组织病理切片可见:A组各时间点肺泡腔清晰,肺泡结构完整,偶见少量炎性细胞。烫伤后第1天,B组及移植组(C、D、E组)肺泡间隔增厚,大量红细胞漏出及炎性细胞浸润。烫伤后第3天,各组肺泡结构较前清晰,炎性细胞浸润及红细胞漏出较第一天减少,与B组相比移植组肺泡结构清晰,间隔变薄,移植组各组间改变不明显。烫伤后第7天,移植组肺组织损伤较B组明显减轻,E组损伤肺组织恢复最为明显。MPO染色显示:与A组相比,阳性细胞数在烫伤后第1天明显增加(P<0.05),但各组之间无明显差异。在烫伤后第3天,与B组相比,移植组阳性细胞数减少明显(P<0.05),E组阳性细胞减少明显(P<0.05);在烫伤后第7天E组阳性细胞数量较其他组显著减少(P<0.05)。CD68染色显示在烫伤后第1天各组阳性细胞显著增多(P>0.05),在烫伤后第3天移植组阳性细胞数减少(P<0.05),但各移植组间无明显差异,烫伤后第7天移植组阳性细胞数量较B组明显减少(P<0.05),E组较C、D组阳性细胞减少有显著差异(P<0.05)。结论:气管内移植HUCMSCs能修复重度烧伤后损伤的肺组织,减少肺组织中性粒细胞及巨噬细胞的浸润,且1×10~6HUCMSCs移植效果更明显。展开更多
The use of umbilical stem cells in tissue engineering is gaining in popularity. Some of the various uses of these umbilical stem cells are highlighted in this review, focusing mainly on their cartilage, bone and neuro...The use of umbilical stem cells in tissue engineering is gaining in popularity. Some of the various uses of these umbilical stem cells are highlighted in this review, focusing mainly on their cartilage, bone and neuronal differentiating abilities. The review will also shed light on the application of these abilities in human clinical trials to repair, protect and treat diseases, e.g. neurodegenerative diseases such as Alzheimer’s and cerebral palsy. A side by side comparison with bone marrow mesenchymal stem cells (current gold standard for tissue engineering) will give a better idea of the viability and efficiency of umbilical stem cell use.展开更多
文摘目的:通过气管内给药的方法比较不同浓度人脐带间充质干细胞气管内移植对重度烧伤致急性肺损伤大鼠的保护作用。方法:建立50%面积全层烫伤大鼠模型,将75只成熟雄性Wistar大鼠随机分为正常对照组(A组)、生理盐水组(B组)、1×10~5HUCMSCs移植组(C)、5×10~5HUCMSCs移植组(D)、1×10~6HUCMSCs移植组(E),每组15只,B组及移植组(C、D、E组)烫伤后立即液体复苏,B组烫伤后气管内滴注0.2 m L生理盐水,移植组气管内滴注不同浓度h UCMSCs,分别在移植后的天第1、3、7天留取大鼠肺组织标本,HE染色观察肺组织病理变化,MPO、CD68免疫组化染色观察肺组织中性粒细胞及肺巨噬细胞阳性表达情况。结果:肺组织病理切片可见:A组各时间点肺泡腔清晰,肺泡结构完整,偶见少量炎性细胞。烫伤后第1天,B组及移植组(C、D、E组)肺泡间隔增厚,大量红细胞漏出及炎性细胞浸润。烫伤后第3天,各组肺泡结构较前清晰,炎性细胞浸润及红细胞漏出较第一天减少,与B组相比移植组肺泡结构清晰,间隔变薄,移植组各组间改变不明显。烫伤后第7天,移植组肺组织损伤较B组明显减轻,E组损伤肺组织恢复最为明显。MPO染色显示:与A组相比,阳性细胞数在烫伤后第1天明显增加(P<0.05),但各组之间无明显差异。在烫伤后第3天,与B组相比,移植组阳性细胞数减少明显(P<0.05),E组阳性细胞减少明显(P<0.05);在烫伤后第7天E组阳性细胞数量较其他组显著减少(P<0.05)。CD68染色显示在烫伤后第1天各组阳性细胞显著增多(P>0.05),在烫伤后第3天移植组阳性细胞数减少(P<0.05),但各移植组间无明显差异,烫伤后第7天移植组阳性细胞数量较B组明显减少(P<0.05),E组较C、D组阳性细胞减少有显著差异(P<0.05)。结论:气管内移植HUCMSCs能修复重度烧伤后损伤的肺组织,减少肺组织中性粒细胞及巨噬细胞的浸润,且1×10~6HUCMSCs移植效果更明显。
文摘The use of umbilical stem cells in tissue engineering is gaining in popularity. Some of the various uses of these umbilical stem cells are highlighted in this review, focusing mainly on their cartilage, bone and neuronal differentiating abilities. The review will also shed light on the application of these abilities in human clinical trials to repair, protect and treat diseases, e.g. neurodegenerative diseases such as Alzheimer’s and cerebral palsy. A side by side comparison with bone marrow mesenchymal stem cells (current gold standard for tissue engineering) will give a better idea of the viability and efficiency of umbilical stem cell use.
