For searching out male sterility-related proteins (polypeptides) in rice(Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice( Wujin2A/R168, Wujin5A/R988) and their paren...For searching out male sterility-related proteins (polypeptides) in rice(Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice( Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorerline R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis(2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimentalmaterials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterileline (Wu-jin2A, WujinSA) by comparing to restorer line (R168, R988) and the first filial (F, )generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pl5.8-6.5, molecular weight 42. 7 X 10~3-66. 2 X 10~3.展开更多
Epstein Barr virus infection is believed to play a role in the development of nasopharyngeal carcinoma. In order to investigate the function of EBV in epithelial cell, proteomic methods were used to find and identify ...Epstein Barr virus infection is believed to play a role in the development of nasopharyngeal carcinoma. In order to investigate the function of EBV in epithelial cell, proteomic methods were used to find and identify the differential proteins and expected to elucidate the mechanism of EBV. Altered protein expressions were found between 293 cell (HEK293) and EBV infected cell (293-EBV). In this study, we separated differential expressed proteins using 2D-DIGE method while matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry (MALDI-TOF-MS) method was used to identify proteins. The results showed that 14 proteins were up regulated and 3 proteins were down regulated in 293-EBV cells. Bioinformatic analysis showed that these proteins are involved in cell proliferation, metastasis, apoptosis, metabolism, and signal transduction. Western blotting analysis was further carried out to verify the MS results. Thus, EBV may exert its functions by mediating differential expression of these proteins.展开更多
This study aimed to study whether the Sortase A(srt A)gene helps mediate coaggregation and co-adherence between Streptococcus mutans(S.mutans)and other salivary bacteria.S.mutans UA159 and srt A-deficient mutant serve...This study aimed to study whether the Sortase A(srt A)gene helps mediate coaggregation and co-adherence between Streptococcus mutans(S.mutans)and other salivary bacteria.S.mutans UA159 and srt A-deficient mutant served as"bait"in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S.mutans with Fusobacterium nucleatum(F.nucleatum),Streptococcus mitis(S.mitis),Streptococcus gordonii(S.gordonii),Streptococcus sanguis(S.sanguis),Actinomyces naeslundii(A.naeslundii)and Lactobacillus.Co-adherence assays were also performed using unfractionated saliva from healthy individuals.Co-adhering partners of S.mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).Both UA159 and its srt A-deficient mutant bound to F.nucleatum but not to any of the other five salivary bacteria.The srt A-deficient mutant showed lower co-adherence with F.nucleatum.The two S.mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria,except that UA159 S.mutans but not the srt A-deficient bound to a Neisseria sp.under the same conditions.Deleting srt A reduces the ability of S.mutans to bind to F.nucleatum,but it does not appear to significantly affect the binding profile of S.mutans to bulk salivary bacteria.展开更多
目的比较轮状病毒(RV)感染与健康儿童肠道菌群结构的差异,为临床治疗提供有价值的参考依据。方法采集20例RV感染及6例健康儿童粪便样品,提取粪便样品中细菌的混合DNA,先通过肠道菌重复性基因内一致性序列(ERIC)-聚合酶链反应(PCR)结合...目的比较轮状病毒(RV)感染与健康儿童肠道菌群结构的差异,为临床治疗提供有价值的参考依据。方法采集20例RV感染及6例健康儿童粪便样品,提取粪便样品中细菌的混合DNA,先通过肠道菌重复性基因内一致性序列(ERIC)-聚合酶链反应(PCR)结合分子杂交技术分析两组儿童之间肠道微生物组成的相似性;再扩增粪便样品中菌群的16 S rDNA基因V3区,利用PCR-TGGE技术分析肠道菌群的组成情况,研究两组儿童肠道微生物菌群组成的差异。结果肠道菌群的组成有很强的宿主专一性。RV感染与健康儿童相比,肠道菌群中糖皮质激素(GC)水平较低的细菌明显减少。微生态制剂治疗组温度梯度凝胶电泳(TGGE)条带数有一个逐渐增加的过程。结论轮状病毒感染时,可致患儿肠道菌群紊乱,但用抗生素治疗将更致肠道微生态失调,不利于患儿肠道菌群恢复,建议临床医师在轮状病毒感染时,慎用抗生素。展开更多
文摘For searching out male sterility-related proteins (polypeptides) in rice(Oryza sativa L. ), we examined the difference of panicle protein (polypeptides) between hybrid rice( Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorerline R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis(2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimentalmaterials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterileline (Wu-jin2A, WujinSA) by comparing to restorer line (R168, R988) and the first filial (F, )generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pl5.8-6.5, molecular weight 42. 7 X 10~3-66. 2 X 10~3.
文摘Epstein Barr virus infection is believed to play a role in the development of nasopharyngeal carcinoma. In order to investigate the function of EBV in epithelial cell, proteomic methods were used to find and identify the differential proteins and expected to elucidate the mechanism of EBV. Altered protein expressions were found between 293 cell (HEK293) and EBV infected cell (293-EBV). In this study, we separated differential expressed proteins using 2D-DIGE method while matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry (MALDI-TOF-MS) method was used to identify proteins. The results showed that 14 proteins were up regulated and 3 proteins were down regulated in 293-EBV cells. Bioinformatic analysis showed that these proteins are involved in cell proliferation, metastasis, apoptosis, metabolism, and signal transduction. Western blotting analysis was further carried out to verify the MS results. Thus, EBV may exert its functions by mediating differential expression of these proteins.
基金supported by grants from the National Natural Science Foundation of China(No.81570974)the Key Project of the Science and Technology Department of Sichuan Province(No.2015JY0260)
文摘This study aimed to study whether the Sortase A(srt A)gene helps mediate coaggregation and co-adherence between Streptococcus mutans(S.mutans)and other salivary bacteria.S.mutans UA159 and srt A-deficient mutant served as"bait"in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S.mutans with Fusobacterium nucleatum(F.nucleatum),Streptococcus mitis(S.mitis),Streptococcus gordonii(S.gordonii),Streptococcus sanguis(S.sanguis),Actinomyces naeslundii(A.naeslundii)and Lactobacillus.Co-adherence assays were also performed using unfractionated saliva from healthy individuals.Co-adhering partners of S.mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).Both UA159 and its srt A-deficient mutant bound to F.nucleatum but not to any of the other five salivary bacteria.The srt A-deficient mutant showed lower co-adherence with F.nucleatum.The two S.mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria,except that UA159 S.mutans but not the srt A-deficient bound to a Neisseria sp.under the same conditions.Deleting srt A reduces the ability of S.mutans to bind to F.nucleatum,but it does not appear to significantly affect the binding profile of S.mutans to bulk salivary bacteria.
文摘目的比较轮状病毒(RV)感染与健康儿童肠道菌群结构的差异,为临床治疗提供有价值的参考依据。方法采集20例RV感染及6例健康儿童粪便样品,提取粪便样品中细菌的混合DNA,先通过肠道菌重复性基因内一致性序列(ERIC)-聚合酶链反应(PCR)结合分子杂交技术分析两组儿童之间肠道微生物组成的相似性;再扩增粪便样品中菌群的16 S rDNA基因V3区,利用PCR-TGGE技术分析肠道菌群的组成情况,研究两组儿童肠道微生物菌群组成的差异。结果肠道菌群的组成有很强的宿主专一性。RV感染与健康儿童相比,肠道菌群中糖皮质激素(GC)水平较低的细菌明显减少。微生态制剂治疗组温度梯度凝胶电泳(TGGE)条带数有一个逐渐增加的过程。结论轮状病毒感染时,可致患儿肠道菌群紊乱,但用抗生素治疗将更致肠道微生态失调,不利于患儿肠道菌群恢复,建议临床医师在轮状病毒感染时,慎用抗生素。
