Objective To establish a fluorescent polymerase chain reaction (F PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application Me...Objective To establish a fluorescent polymerase chain reaction (F PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application Methods The primers and the fluorescence labeled probe were designed and synthesized according to the published sequence of the SARS associated coronavirus genes A F PCR diagnosis kit for detecting the coronavirus was developed, and 115 clinical nasopharyngeal gargling liquid samples were tested Results The sequence of PCR amplified products completely matched the related sequence of the SARS associated coronavirus genome Forty nine out of 67 samples from identified SARS patients and 8 of 18 samples from persons having close contact with SARS patients showed positive results All 30 samples from healthy controls were negative Conclusion The F PCR method established may be a rapid, accurate and efficient way for screening and for the early diagnosis of SARS patients展开更多
目的:探讨中药冰块含漱+耳穴贴压对甲状腺术后疼痛的干预价值。方法:选择2018年1月-2020年1月笔者所在医院收治的72例甲状腺手术患者,按照患者接受手术的先后顺序进行编号,编号奇数者设为观察组,编号偶数者设为对照组,每组36例,对照组...目的:探讨中药冰块含漱+耳穴贴压对甲状腺术后疼痛的干预价值。方法:选择2018年1月-2020年1月笔者所在医院收治的72例甲状腺手术患者,按照患者接受手术的先后顺序进行编号,编号奇数者设为观察组,编号偶数者设为对照组,每组36例,对照组在术毕返回病房2 h后采用自制中药冰块进行含漱治疗,观察组同时配合耳穴贴压疗法,分别采用数字疼痛分级法(NRS)、匹兹堡睡眠评价量表(PSQI)评估两组术后6、24 h疼痛程度与睡眠质量,同时比较术后住院时间。结果:观察组术后6、24 h NRS评分均低于对照组,术后24、48 h PSQI评分均低于对照组,术后住院时间短于对照组,差异均有统计学意义(P<0.05)。结论:中药冰块含漱+耳穴贴压对甲状腺术后疼痛有显著缓解作用,改善睡眠,可缩短住院时间加速康复进程,值得临床推广。展开更多
文摘Objective To establish a fluorescent polymerase chain reaction (F PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application Methods The primers and the fluorescence labeled probe were designed and synthesized according to the published sequence of the SARS associated coronavirus genes A F PCR diagnosis kit for detecting the coronavirus was developed, and 115 clinical nasopharyngeal gargling liquid samples were tested Results The sequence of PCR amplified products completely matched the related sequence of the SARS associated coronavirus genome Forty nine out of 67 samples from identified SARS patients and 8 of 18 samples from persons having close contact with SARS patients showed positive results All 30 samples from healthy controls were negative Conclusion The F PCR method established may be a rapid, accurate and efficient way for screening and for the early diagnosis of SARS patients
文摘目的:探讨中药冰块含漱+耳穴贴压对甲状腺术后疼痛的干预价值。方法:选择2018年1月-2020年1月笔者所在医院收治的72例甲状腺手术患者,按照患者接受手术的先后顺序进行编号,编号奇数者设为观察组,编号偶数者设为对照组,每组36例,对照组在术毕返回病房2 h后采用自制中药冰块进行含漱治疗,观察组同时配合耳穴贴压疗法,分别采用数字疼痛分级法(NRS)、匹兹堡睡眠评价量表(PSQI)评估两组术后6、24 h疼痛程度与睡眠质量,同时比较术后住院时间。结果:观察组术后6、24 h NRS评分均低于对照组,术后24、48 h PSQI评分均低于对照组,术后住院时间短于对照组,差异均有统计学意义(P<0.05)。结论:中药冰块含漱+耳穴贴压对甲状腺术后疼痛有显著缓解作用,改善睡眠,可缩短住院时间加速康复进程,值得临床推广。
