Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence i...Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence in Arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of GLABRA2 （GL2） controlling trichome cell differentiation; it is also assumed that TRIPTYCHON （TRY） and CAPRICE （CPC） counteract the complex formation in neighboring cells. Here, the homologs GaMYB23 （a homolog of GL1）, GaDEL65 （a homolog of GL3）, GaTTG1, GaCPC and GaTRY were identified in Gossypium arboreum. We show that GaMYB23 can bind to and activate the promoters of GaCPC, GaGL2 and GaTRY, and that GaMYB23, GaTRY and GaTTG1 could interact with GaDEL65 in yeast and in planta. In situ analysis showed that GaMYB23, GaGL2, GaDEL65, and GaTRY were predominantly expressed in fuzz fiber, but GaTRY proteins were primarily found in undeveloped epidermal cells. A G. arboreum fuzzless mutant with consistently high level GaMYB23 transcript has lost the detectable GaMYB23-promoter of GaGL2 complex, corresponding to sharply reduced transcription of GaGL2. Our results support that cotton homologs to the genetic molecules regulating Arabidopsis trichome differentiation interacted in the epidermis of ovules and the redundant GaMYB23 serves as a negative regulator in fuzz-fiber patterning.展开更多
Various fiber mutants of cotton have been reported since 1920. Two of the best characterized mutants are the naked seed loci, N1N1 and n2n2. Recently, a naked-tufted mutant called 9023 nt4 was developed from the culti...Various fiber mutants of cotton have been reported since 1920. Two of the best characterized mutants are the naked seed loci, N1N1 and n2n2. Recently, a naked-tufted mutant called 9023 nt4 was developed from the cultivar SC 9023 through chemical mutagenesis. The mutant was tested to determine if it was allelic to either N1, or n2 or was a unique mutant in a new uncharacterized locus. In 1999, one M3 plant from SC 9023 with tufted partially naked seed coat (fuzzless) was identified. In 2004, 2006, and 2007 (Lubbock, TX), 2007 (College Station, TX), and 2011 (Stoneville, MS), the homozygous naked seed mutant was evaluated with the non-mutated wild type parent (SC 9023) in rep-licated trials for agronomic and fiber traits. Crosses between the mutant and the wild type was made at Stoneville, MS in 2009. The F2 of this cross segregated in a 3 fuzzy: 1 fuzzless ratio indicating that the fuzzless trait in the mutant is controlled by a recessive locus. Allelism tests with N1N1, n2n2 and n3n3, lint percent, and fiber trait data indicated that the new locus in the mutant differs from the previously characterized fuzzless seed alleles in that it does not appear to decrease lint percent. We have putatively designated this gene nt4nt4 .展开更多
CAP,an adenylyl cyclase-associated protein,is predicted to be involved in cytoskeletal organization and signal transduction.Recently,we found that CAP may play an important role in fuzz-like fiber cell initiation in c...CAP,an adenylyl cyclase-associated protein,is predicted to be involved in cytoskeletal organization and signal transduction.Recently,we found that CAP may play an important role in fuzz-like fiber cell initiation in cotton.For the further research,we isolated two CAP homologues from wild展开更多
基金supported by grants from the National Key Basic Research and Development Program of China(2010CB12600)
文摘Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence in Arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of GLABRA2 （GL2） controlling trichome cell differentiation; it is also assumed that TRIPTYCHON （TRY） and CAPRICE （CPC） counteract the complex formation in neighboring cells. Here, the homologs GaMYB23 （a homolog of GL1）, GaDEL65 （a homolog of GL3）, GaTTG1, GaCPC and GaTRY were identified in Gossypium arboreum. We show that GaMYB23 can bind to and activate the promoters of GaCPC, GaGL2 and GaTRY, and that GaMYB23, GaTRY and GaTTG1 could interact with GaDEL65 in yeast and in planta. In situ analysis showed that GaMYB23, GaGL2, GaDEL65, and GaTRY were predominantly expressed in fuzz fiber, but GaTRY proteins were primarily found in undeveloped epidermal cells. A G. arboreum fuzzless mutant with consistently high level GaMYB23 transcript has lost the detectable GaMYB23-promoter of GaGL2 complex, corresponding to sharply reduced transcription of GaGL2. Our results support that cotton homologs to the genetic molecules regulating Arabidopsis trichome differentiation interacted in the epidermis of ovules and the redundant GaMYB23 serves as a negative regulator in fuzz-fiber patterning.
文摘Various fiber mutants of cotton have been reported since 1920. Two of the best characterized mutants are the naked seed loci, N1N1 and n2n2. Recently, a naked-tufted mutant called 9023 nt4 was developed from the cultivar SC 9023 through chemical mutagenesis. The mutant was tested to determine if it was allelic to either N1, or n2 or was a unique mutant in a new uncharacterized locus. In 1999, one M3 plant from SC 9023 with tufted partially naked seed coat (fuzzless) was identified. In 2004, 2006, and 2007 (Lubbock, TX), 2007 (College Station, TX), and 2011 (Stoneville, MS), the homozygous naked seed mutant was evaluated with the non-mutated wild type parent (SC 9023) in rep-licated trials for agronomic and fiber traits. Crosses between the mutant and the wild type was made at Stoneville, MS in 2009. The F2 of this cross segregated in a 3 fuzzy: 1 fuzzless ratio indicating that the fuzzless trait in the mutant is controlled by a recessive locus. Allelism tests with N1N1, n2n2 and n3n3, lint percent, and fiber trait data indicated that the new locus in the mutant differs from the previously characterized fuzzless seed alleles in that it does not appear to decrease lint percent. We have putatively designated this gene nt4nt4 .
基金The authers sre greafiy indebted to Dr.Russell J.KohelUSDA ARS+5 种基金Southern Crops Research LaboratoryCollege StationTX77845USAfor providing TM-1T586 and n2 cotton seeds and giving the instructive help
文摘CAP,an adenylyl cyclase-associated protein,is predicted to be involved in cytoskeletal organization and signal transduction.Recently,we found that CAP may play an important role in fuzz-like fiber cell initiation in cotton.For the further research,we isolated two CAP homologues from wild
