We have cloned the replicative form of the Periplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome consists of an internal u...We have cloned the replicative form of the Periplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome consists of an internal unique sequence flanked by inverted terminal repeats (201 nt). The first 122 nt at the 5’end and the terminal 122 nt at the 3’end of both plus and minus strands can fold into a typical hairpin structure. The genome contains seven major open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5’half of the plus strand, whereas the others span the 5’ half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Computer analysis of sequence homologies with other parvoviruses suggests that the plus strand of Pf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins.展开更多
The vacuolar (H+)-ATPases (V-ATPases) are ATP-driven proton pumps with multiple functions in many organisms. In this study, we performed structural and functional analysis of vha55 gene that encodes V-ATPase subunit B...The vacuolar (H+)-ATPases (V-ATPases) are ATP-driven proton pumps with multiple functions in many organisms. In this study, we performed structural and functional analysis of vha55 gene that encodes V-ATPase subunit B in the smokybrown cockroach Periplaneta fuliginosa (Blattodea). We observed a high homology score of the deduced amino acid sequences between 10 species in seven orders. RNAi of the vha55 gene in R fuliginosa caused nymphal/nymphal molting defects with incomplete shedding of old cuticles, growth inhibition, as well as bent and wrinkled cuticles of thoraxes and abdominal segments. Since growth inhibition caused by vha55 RNAi did not interfere in the commencement of cockroach molting, molting timing and body growth might be controlled by independent mechanism. Our study suggested V-ATPases might be a good candidate molecule for evolutionary and developmental studies of insect molting.展开更多
目的观察对家属楼居民户蟑螂的杀灭效果。方法用晔康杀蟑胶饵对单位家属楼室内,用拜力坦热烟雾对排水沟、暖气沟进行现场灭蟑实验。结果试验前现场室内蟑密度平均为15只/15 m in,施药后,10、20、30 d,平均密度分别为1.5,0,0只/15 m in,...目的观察对家属楼居民户蟑螂的杀灭效果。方法用晔康杀蟑胶饵对单位家属楼室内,用拜力坦热烟雾对排水沟、暖气沟进行现场灭蟑实验。结果试验前现场室内蟑密度平均为15只/15 m in,施药后,10、20、30 d,平均密度分别为1.5,0,0只/15 m in,杀灭率分别为90.0%,100%,100%。经6个月观察各房间均未见活蟑。结论晔康杀蟑胶饵对家属楼居民户灭蟑效果理想,拜力坦热烟雾对排水沟、暖气沟灭蟑效果理想,并能达到较长时间的控制目的。展开更多
文摘We have cloned the replicative form of the Periplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome consists of an internal unique sequence flanked by inverted terminal repeats (201 nt). The first 122 nt at the 5’end and the terminal 122 nt at the 3’end of both plus and minus strands can fold into a typical hairpin structure. The genome contains seven major open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5’half of the plus strand, whereas the others span the 5’ half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Computer analysis of sequence homologies with other parvoviruses suggests that the plus strand of Pf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins.
文摘The vacuolar (H+)-ATPases (V-ATPases) are ATP-driven proton pumps with multiple functions in many organisms. In this study, we performed structural and functional analysis of vha55 gene that encodes V-ATPase subunit B in the smokybrown cockroach Periplaneta fuliginosa (Blattodea). We observed a high homology score of the deduced amino acid sequences between 10 species in seven orders. RNAi of the vha55 gene in R fuliginosa caused nymphal/nymphal molting defects with incomplete shedding of old cuticles, growth inhibition, as well as bent and wrinkled cuticles of thoraxes and abdominal segments. Since growth inhibition caused by vha55 RNAi did not interfere in the commencement of cockroach molting, molting timing and body growth might be controlled by independent mechanism. Our study suggested V-ATPases might be a good candidate molecule for evolutionary and developmental studies of insect molting.
文摘目的观察对家属楼居民户蟑螂的杀灭效果。方法用晔康杀蟑胶饵对单位家属楼室内,用拜力坦热烟雾对排水沟、暖气沟进行现场灭蟑实验。结果试验前现场室内蟑密度平均为15只/15 m in,施药后,10、20、30 d,平均密度分别为1.5,0,0只/15 m in,杀灭率分别为90.0%,100%,100%。经6个月观察各房间均未见活蟑。结论晔康杀蟑胶饵对家属楼居民户灭蟑效果理想,拜力坦热烟雾对排水沟、暖气沟灭蟑效果理想,并能达到较长时间的控制目的。
