This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular ...This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular and smooth with intact intima,myometrium and adventitia.The blood vessel was wrapped with the tissue and there was no thrombosis in the lumen.In the DVT model,the wall was uneven with thicken intima,myometrium and adventitia.After treated with fucoidans LF1 and LF2,the thrombus was dissolved and the blood vessel was recanalized.Compared with the control group,the ROS content,ET-1 and VWF content and the expression of PKC-βand NF-κB in the model were significantly higher(P<0.05);these levels were significantly reduced following treatments with LF2 and LF1.Compared with H_(2)O_(2)treated-HUVECs,combined LF1 and LF2 treatment resulted in significant decrease in the expression of PKC-β,NF-κB,VWF and TM protein(P<0.05).It is clear that LF1 and LF2 reduces DVT-induced ET-1,VWF and TM expressions and production of ROS,thus inhibiting the activation of PKC-β/NF-κB signal pathway and the activation of coagulation system and ultimately reducing the formation of venous thrombus.展开更多
采用超声波辅助过氧化氢的方法降解岩藻聚糖,采用膜分离制备分子量>50 ku和<50 ku 2个组分的低分子量岩藻聚糖组分,并以未降解的岩藻聚糖为对照测定其抗菌活性。通过多糖降解率、硫酸基团保留率和抗菌生物学活性的比较研究证明,...采用超声波辅助过氧化氢的方法降解岩藻聚糖,采用膜分离制备分子量>50 ku和<50 ku 2个组分的低分子量岩藻聚糖组分,并以未降解的岩藻聚糖为对照测定其抗菌活性。通过多糖降解率、硫酸基团保留率和抗菌生物学活性的比较研究证明,超声波辅助降解2 h时的降解效果较好,此时的水解率为30.73%,硫酸基团保留率在24.71%,抑菌圈直径为25 mm。此条件下所制备的低分子量岩藻聚糖具有较好的理化性质及抗菌生物学活性。展开更多
In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF)...In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF), and Ecklonia maxima(EMF). LJF was less in sulfate content(14.16%) and more in galactose and mannose content(1.08 and 0.68) than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans(1.21–1.41). AMF(162 kDa) and EMF(150 kDa) were the first two largest in molecular weight, which were followed by LJP(126 kDa), LNF(113 kDa) and LTF(105 kDa). The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity.展开更多
Heparan sulfate glycosaminoglycans are key players of tissue repair and can be regarded as useful compounds for regenerative medicines.Unfortunately,their therapeutic uses face many technical,industrial,and regulatory...Heparan sulfate glycosaminoglycans are key players of tissue repair and can be regarded as useful compounds for regenerative medicines.Unfortunately,their therapeutic uses face many technical,industrial,and regulatory hurdles due to their animal origin.So,some non-animal sulfated polysaccharides mimic heparan sulfate properties and offer interesting solutions to replace them.Among them,dextran derivatives,seaweed polysaccharides,or marine bacterial polysaccharides are the best known and have demonstrated their pro-regenerative capabilities by promoting both extracellular matrix structuring and angiogenesis and limiting degenerative processes such as inflammatory cell migration or tissue proteolysis.These polysaccharides have also shown their ability to specifically promote osteoblastic differentiation and bone wound healing.Furthermore,recent works shows that heparan-mimetics can be used as an additive to improve the cytocompatibility of bone substitutes commonly used in periodontal surgery.The use of these polysaccharides can be regarded as a clever approach to improve the biointegration of bone substitutes.展开更多
基金supported by the Special Fund for Clinical Scientific Research of Shandong Medical Association(No.YXH2020ZX058).
文摘This study was carried out explore the mechanism underlying the inhibition of platelet activation by kelp fucoidans in deep venous thrombosis(DVT)mouse.In the control and sham mice,the walls of deep vein were regular and smooth with intact intima,myometrium and adventitia.The blood vessel was wrapped with the tissue and there was no thrombosis in the lumen.In the DVT model,the wall was uneven with thicken intima,myometrium and adventitia.After treated with fucoidans LF1 and LF2,the thrombus was dissolved and the blood vessel was recanalized.Compared with the control group,the ROS content,ET-1 and VWF content and the expression of PKC-βand NF-κB in the model were significantly higher(P<0.05);these levels were significantly reduced following treatments with LF2 and LF1.Compared with H_(2)O_(2)treated-HUVECs,combined LF1 and LF2 treatment resulted in significant decrease in the expression of PKC-β,NF-κB,VWF and TM protein(P<0.05).It is clear that LF1 and LF2 reduces DVT-induced ET-1,VWF and TM expressions and production of ROS,thus inhibiting the activation of PKC-β/NF-κB signal pathway and the activation of coagulation system and ultimately reducing the formation of venous thrombus.
基金supported by the Ocean Public Welfare Scientific Research Project, State Ocean Administration of China (No. 201105029-9)
文摘In this study, we evaluated the chemical property and antioxidant activity of fucoidans isolated from brown algae, Laminaria japonica(LJF), Lessonia nigrescens(LNF), Lessonia trabeculata(LTF), Ascophyllum mackaii(AMF), and Ecklonia maxima(EMF). LJF was less in sulfate content(14.16%) and more in galactose and mannose content(1.08 and 0.68) than the documented early. EMF contained 20%–30% of sulfate and fucose, 0.97 in molar ratio which was lower than that of sulfate to other four fucoidans(1.21–1.41). AMF(162 kDa) and EMF(150 kDa) were the first two largest in molecular weight, which were followed by LJP(126 kDa), LNF(113 kDa) and LTF(105 kDa). The fucoidans isolated these algae showed a wide range of antioxidant activity in vitro. It was found that the reducing power of the isolated fucoidans was positively correlated with their sulfate content and molecular weight. In addition, LNF and LTF at low concentrations exhibited high superoxide and hydroxyl radical scavenging activity. This demonstrated that low molecular weight fucoidans may perform a high antioxidant activity.
基金"This work was partially supported by the“PHC Alliance”programme“Cedre”(project number:44502ND),funded by the French Ministry for Europe and Foreign Affairs,the French Ministry for Higher Education,Research and Innovation and the Lebanon Ministry of Education&Higher Education and National Council for Scientific Research-Lebanon.This work was supported by Both Association de l’Ecole de Biologie Industrielle,Cergy,France and Saint Joseph University of Beirut,Lebanon.
文摘Heparan sulfate glycosaminoglycans are key players of tissue repair and can be regarded as useful compounds for regenerative medicines.Unfortunately,their therapeutic uses face many technical,industrial,and regulatory hurdles due to their animal origin.So,some non-animal sulfated polysaccharides mimic heparan sulfate properties and offer interesting solutions to replace them.Among them,dextran derivatives,seaweed polysaccharides,or marine bacterial polysaccharides are the best known and have demonstrated their pro-regenerative capabilities by promoting both extracellular matrix structuring and angiogenesis and limiting degenerative processes such as inflammatory cell migration or tissue proteolysis.These polysaccharides have also shown their ability to specifically promote osteoblastic differentiation and bone wound healing.Furthermore,recent works shows that heparan-mimetics can be used as an additive to improve the cytocompatibility of bone substitutes commonly used in periodontal surgery.The use of these polysaccharides can be regarded as a clever approach to improve the biointegration of bone substitutes.
