目的:建立一种同时测定黄连解毒汤配方颗粒中4种成分(栀子苷、黄芩苷、盐酸巴马汀、盐酸小檗碱)含量的HPLC方法,对黄连解毒汤配方颗粒和传统汤剂的主要色谱峰及成分含量进行比较。方法:采用菲罗门Gemini 5u C18110A色谱柱(250mm×4....目的:建立一种同时测定黄连解毒汤配方颗粒中4种成分(栀子苷、黄芩苷、盐酸巴马汀、盐酸小檗碱)含量的HPLC方法,对黄连解毒汤配方颗粒和传统汤剂的主要色谱峰及成分含量进行比较。方法:采用菲罗门Gemini 5u C18110A色谱柱(250mm×4.6mm,5μm),以乙腈为流动相A,以每100m L水中含1m L p H=6.0的醋酸-醋酸铵缓冲溶液为流动相B;梯度洗脱,体积流量为1.0m L/min,检测波长260nm,柱温30℃。结果:在39min内黄连解毒汤配方颗粒中栀子苷、黄芩苷、盐酸巴马汀、盐酸小檗碱4种成分完全分离;回归方程显示4种成分的峰面积与其浓度呈良好的线性关系;4种成分的平均加样回收率(n=6)为95.02%~104.57%,RSD均小于3.0%;黄连解毒汤配方颗粒与传统汤剂的主要色谱峰的峰形峰位一致,成分含量相当。结论:该方法简单快捷,灵敏度高,稳定、重复性好,可用于黄连解毒汤配方颗粒的质量控制,黄连解毒汤配方颗粒与传统汤剂的主要成分一致,可为其替代传统汤剂作为理论依据。展开更多
目的同时测定厚朴养胃疏肝汤中的两个指标性成分,研究该制剂在采用配方颗粒调配时与传统水煎剂临床用量的差异。方法取厚朴养胃疏肝汤各药味配方颗粒或饮片,配方颗粒直接加水调配,饮片加水煎煮;所得药液经前处理后进高效液相色谱仪分析...目的同时测定厚朴养胃疏肝汤中的两个指标性成分,研究该制剂在采用配方颗粒调配时与传统水煎剂临床用量的差异。方法取厚朴养胃疏肝汤各药味配方颗粒或饮片,配方颗粒直接加水调配,饮片加水煎煮;所得药液经前处理后进高效液相色谱仪分析,使用Waters Xbridge C 18色谱柱(4.6×250mm,5μm),以0.1%磷酸-乙腈为流动相,梯度洗脱,流速1.0mL·min^-1,检测波长254nm,柱温30℃,进样量10μL;共使用10批配方颗粒/饮片配制厚朴养胃疏肝汤。结果10批配方颗粒配制的药液中,厚朴酚的含量为0.0201~0.0343mg·mL^-1,相当于饮片含量的92.10%~121.96%;茯苓酸B的含量为0.1340~0.1615mg·mL^-1,相当于饮片含量的90.90%~116.81%。结论配方颗粒调制的朴养胃疏肝汤与饮片水煎液无明显差异,可用该方法替代原有工艺。展开更多
[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCE...[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm,5μm);mobile phase,acetonitrile-0.1%phosphoric acid;gradient elution;detection wavelength,280 nm;flow rate,1.0 mL/min;and column temperature,30℃.The correlation between the decoction pieces,standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms.[Results]The fingerprint chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae showed five common peaks,with good correlation.Among the five common peaks,four of them were tanshinol,protocatechuic acid,caffeic acid and rosmarinic acid.[Conclusions]The main chemical constituents of decoction pieces,standard decoction and formula granules of Spica Prunellae are basically the same.The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.展开更多
文摘目的:建立一种同时测定黄连解毒汤配方颗粒中4种成分(栀子苷、黄芩苷、盐酸巴马汀、盐酸小檗碱)含量的HPLC方法,对黄连解毒汤配方颗粒和传统汤剂的主要色谱峰及成分含量进行比较。方法:采用菲罗门Gemini 5u C18110A色谱柱(250mm×4.6mm,5μm),以乙腈为流动相A,以每100m L水中含1m L p H=6.0的醋酸-醋酸铵缓冲溶液为流动相B;梯度洗脱,体积流量为1.0m L/min,检测波长260nm,柱温30℃。结果:在39min内黄连解毒汤配方颗粒中栀子苷、黄芩苷、盐酸巴马汀、盐酸小檗碱4种成分完全分离;回归方程显示4种成分的峰面积与其浓度呈良好的线性关系;4种成分的平均加样回收率(n=6)为95.02%~104.57%,RSD均小于3.0%;黄连解毒汤配方颗粒与传统汤剂的主要色谱峰的峰形峰位一致,成分含量相当。结论:该方法简单快捷,灵敏度高,稳定、重复性好,可用于黄连解毒汤配方颗粒的质量控制,黄连解毒汤配方颗粒与传统汤剂的主要成分一致,可为其替代传统汤剂作为理论依据。
文摘目的同时测定厚朴养胃疏肝汤中的两个指标性成分,研究该制剂在采用配方颗粒调配时与传统水煎剂临床用量的差异。方法取厚朴养胃疏肝汤各药味配方颗粒或饮片,配方颗粒直接加水调配,饮片加水煎煮;所得药液经前处理后进高效液相色谱仪分析,使用Waters Xbridge C 18色谱柱(4.6×250mm,5μm),以0.1%磷酸-乙腈为流动相,梯度洗脱,流速1.0mL·min^-1,检测波长254nm,柱温30℃,进样量10μL;共使用10批配方颗粒/饮片配制厚朴养胃疏肝汤。结果10批配方颗粒配制的药液中,厚朴酚的含量为0.0201~0.0343mg·mL^-1,相当于饮片含量的92.10%~121.96%;茯苓酸B的含量为0.1340~0.1615mg·mL^-1,相当于饮片含量的90.90%~116.81%。结论配方颗粒调制的朴养胃疏肝汤与饮片水煎液无明显差异,可用该方法替代原有工艺。
基金Supported by Scientific Research and Technology Development Project of Nanning City(20173158-5).
文摘[Objectives]This study aimed to establish HPLC chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae.[Methods]The chromatographic conditions were as follows:column,SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm,5μm);mobile phase,acetonitrile-0.1%phosphoric acid;gradient elution;detection wavelength,280 nm;flow rate,1.0 mL/min;and column temperature,30℃.The correlation between the decoction pieces,standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms.[Results]The fingerprint chromatograms of decoction pieces,standard decoction and formula granules of Spica Prunellae showed five common peaks,with good correlation.Among the five common peaks,four of them were tanshinol,protocatechuic acid,caffeic acid and rosmarinic acid.[Conclusions]The main chemical constituents of decoction pieces,standard decoction and formula granules of Spica Prunellae are basically the same.The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.
