The chemical constituents of fistular onion stalk obtained by supercritical CO2 extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four...The chemical constituents of fistular onion stalk obtained by supercritical CO2 extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained. On the basis of the spectral data, they were structurally identified as （＋）-catechin, （-）-epieatechin, astragalin, and 3-O-β-D（2-O-β-D-glu- copyranosyl）-glucopyranosides of kaempferol.展开更多
[ Objective] Fourier transform infrared spectroscopy was used to study fistular onion （Alliumfistulosum） and garlic （Allium sativum） rust leaves, so as to explore the application potential of mid-infrared spectros...[ Objective] Fourier transform infrared spectroscopy was used to study fistular onion （Alliumfistulosum） and garlic （Allium sativum） rust leaves, so as to explore the application potential of mid-infrared spectroscopy in the diagnosis of crop disease. [ Method] The normal green leaves and rust leaves of fistular onion and garlic were tested using fourier transform infrared spectroscopy, and their spectral differences were analyzed. [ Result] The infrared spectra of green leaves of fistular onion and garlic are mainly composed of the vibrational bands of polysaccharides, protein and ester. Normal and rust leaves exhibited differences in the fin- gerprint interval of 1 800 -900 cm-1 , in which the band at 1 640 cm-1 in the spectrum of flstular onion rust leaf became stronger, and the band at 1 103 cm-1 was not obvious compared with the spectrum of normal leaf. The absorbance ratios A1640/A1063, A1640/A1736, A1640/A2924, and A1063/A2924 of onion rust leaf were larger than the corresponding ratios A1638/A1 059, A1738/A1 38, A2921/A1638, and A2 92l/A1059 in the spectrum of normal leaf. The band of polysaccharides at 1 056 cm-1 in the spectrmn of garlic normal leaf was the strongest in fingerprint area, while the band at 1 634 cm-1 in the spectrum of garlic rust leaf was the strongest in that are- a. The absorbance ratios A1634/A1069 , A1634/A1099 , A1409/A2923 , and A1634/A737 of garlic rust leaf were larger than the corresponding ratios A1627/A1056 , A1623/A1104 , A1411/A2920 and A1627/A1740 of normal leaves. While the ratios A1634/A2923, A1059/A2923, and A1737/A2923 of garlic rust leaf were less than the corresponding values of A1627/A2920, A1056/A2920, and A1 740/A2920 of normal leaf. [ Conclusion] Rust leaf and normal leaf could be distinguished according to the differences in infrared spectra, and infrared spectroscopy could be developed as the detection method for crop diseases.展开更多
目的建立一种较为简单的测定葱白提取物中甾体总皂苷含量的方法。方法以薯蓣皂苷元作为对照品,通过正交实验对香草醛比色法实验条件进行优化,确定比色条件为挥干溶剂后加0.5%香草醛-冰醋酸0.2 mL和高氯酸1.0 mL,于80℃水浴30 m in。结...目的建立一种较为简单的测定葱白提取物中甾体总皂苷含量的方法。方法以薯蓣皂苷元作为对照品,通过正交实验对香草醛比色法实验条件进行优化,确定比色条件为挥干溶剂后加0.5%香草醛-冰醋酸0.2 mL和高氯酸1.0 mL,于80℃水浴30 m in。结果测得葱白提取物中总皂苷含量为11.50%,平均加样回收率为101.30%,RSD为2.08%(n=6)。结论采用香草醛显色法测定葱白提取物中总皂苷含量操作简便,结果可靠。展开更多
基金supported by a grant from a National Program of Science and Research of China (No. 2006BAI09B08-07)
文摘The chemical constituents of fistular onion stalk obtained by supercritical CO2 extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained. On the basis of the spectral data, they were structurally identified as （＋）-catechin, （-）-epieatechin, astragalin, and 3-O-β-D（2-O-β-D-glu- copyranosyl）-glucopyranosides of kaempferol.
基金Supported by National Natural Science Foundation of China(30960179)~~
文摘[ Objective] Fourier transform infrared spectroscopy was used to study fistular onion （Alliumfistulosum） and garlic （Allium sativum） rust leaves, so as to explore the application potential of mid-infrared spectroscopy in the diagnosis of crop disease. [ Method] The normal green leaves and rust leaves of fistular onion and garlic were tested using fourier transform infrared spectroscopy, and their spectral differences were analyzed. [ Result] The infrared spectra of green leaves of fistular onion and garlic are mainly composed of the vibrational bands of polysaccharides, protein and ester. Normal and rust leaves exhibited differences in the fin- gerprint interval of 1 800 -900 cm-1 , in which the band at 1 640 cm-1 in the spectrum of flstular onion rust leaf became stronger, and the band at 1 103 cm-1 was not obvious compared with the spectrum of normal leaf. The absorbance ratios A1640/A1063, A1640/A1736, A1640/A2924, and A1063/A2924 of onion rust leaf were larger than the corresponding ratios A1638/A1 059, A1738/A1 38, A2921/A1638, and A2 92l/A1059 in the spectrum of normal leaf. The band of polysaccharides at 1 056 cm-1 in the spectrmn of garlic normal leaf was the strongest in fingerprint area, while the band at 1 634 cm-1 in the spectrum of garlic rust leaf was the strongest in that are- a. The absorbance ratios A1634/A1069 , A1634/A1099 , A1409/A2923 , and A1634/A737 of garlic rust leaf were larger than the corresponding ratios A1627/A1056 , A1623/A1104 , A1411/A2920 and A1627/A1740 of normal leaves. While the ratios A1634/A2923, A1059/A2923, and A1737/A2923 of garlic rust leaf were less than the corresponding values of A1627/A2920, A1056/A2920, and A1 740/A2920 of normal leaf. [ Conclusion] Rust leaf and normal leaf could be distinguished according to the differences in infrared spectra, and infrared spectroscopy could be developed as the detection method for crop diseases.
文摘目的建立一种较为简单的测定葱白提取物中甾体总皂苷含量的方法。方法以薯蓣皂苷元作为对照品,通过正交实验对香草醛比色法实验条件进行优化,确定比色条件为挥干溶剂后加0.5%香草醛-冰醋酸0.2 mL和高氯酸1.0 mL,于80℃水浴30 m in。结果测得葱白提取物中总皂苷含量为11.50%,平均加样回收率为101.30%,RSD为2.08%(n=6)。结论采用香草醛显色法测定葱白提取物中总皂苷含量操作简便,结果可靠。
