According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (...According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.展开更多
In recent years,Pickering emulsions and their applications have attracted a great deal of attention due to their special features,which include easy preparation and enhanced stability.In contrast to classical emulsion...In recent years,Pickering emulsions and their applications have attracted a great deal of attention due to their special features,which include easy preparation and enhanced stability.In contrast to classical emulsions,in Pickering emulsions,solid microparticles or nanoparticles that localize at the interface between liquids are used as stabilizers,instead of surfactants,to enhance the droplet lifetime.Furthermore,Pickering emulsions show higher stability,lower toxicity,and stimuli-responsiveness,compared with emulsions that are stabilized by surfactants.Therefore,they can be considered attractive components for various uses,such as photocatalysis and the preparation of new materials.Moreover,the nanoparticle morphology strongly influences Pickering emulsion stability as well as the potential utilization of such emulsions.Here,we review recent findings concerning Pickering emulsions,with a particular focus on how the nanoparticles morphology(i.e.,cube,ellipsoid,nanosheet,sphere,cylinder,rod,peanut)influences the type and stability of such emulsions,and their current applications in different fields such as antibacterial activity,protein recognition,catalysis,photocatalysis,and water purification.展开更多
A novel polymer/SiO2 hybrid emulsion(PAES)was prepared by directly mixing colloidal silica with polyacrylate emulsion(PAE)modified by a saline coupling agent.The sol-gel-derived thin films were obtained by addition of...A novel polymer/SiO2 hybrid emulsion(PAES)was prepared by directly mixing colloidal silica with polyacrylate emulsion(PAE)modified by a saline coupling agent.The sol-gel-derived thin films were obtained by addition of co-solvents into the PAES.The effects ofγ-methacryloxypropyltrimethoxysilane(KH-570)content and co-solvent on the properties of PAES films were investigated.Dynamic laser scattering(DLS)data indicate that the average diameter of PAES(96 nm)is slightly larger than that of PAE(89 nm).Transmission electron microscopy (TEM)photo discloses that colloidal silica particles are dispersed uniformly around polyacrylate particles and some of the colloidal silica particles are adsorbed on the surface of PAE particles.The crosslinking degree data and Fourier transform infrared(FT-IR)spectra confirm that the chemical structure of the PAES is changed to form Si-O-Si-polymer crosslinking networks during the film formation.Atomic force microscope(AFM)photos show the solvent induced sol-gel process of colloidal silica and the Si-based polymer distribution on the film surface of the dried PAES.Thermogravimetric analysis(TGA)curves demonstrate that the PAES films display much better thermal stability than PAE.展开更多
基金We would like to thank Peifang Ping,BaozhenPeng and Dr.Xinxiu Yang for their helps in im-munization and ELISA.This work was funded bythe National Natural Sciences Foundation of China,No.39893320,the"973"Basic Research FundingScheme of China(G 199905590
文摘According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.
文摘In recent years,Pickering emulsions and their applications have attracted a great deal of attention due to their special features,which include easy preparation and enhanced stability.In contrast to classical emulsions,in Pickering emulsions,solid microparticles or nanoparticles that localize at the interface between liquids are used as stabilizers,instead of surfactants,to enhance the droplet lifetime.Furthermore,Pickering emulsions show higher stability,lower toxicity,and stimuli-responsiveness,compared with emulsions that are stabilized by surfactants.Therefore,they can be considered attractive components for various uses,such as photocatalysis and the preparation of new materials.Moreover,the nanoparticle morphology strongly influences Pickering emulsion stability as well as the potential utilization of such emulsions.Here,we review recent findings concerning Pickering emulsions,with a particular focus on how the nanoparticles morphology(i.e.,cube,ellipsoid,nanosheet,sphere,cylinder,rod,peanut)influences the type and stability of such emulsions,and their current applications in different fields such as antibacterial activity,protein recognition,catalysis,photocatalysis,and water purification.
基金Supported by the Program for New Century Excellent Talents in University（NCET-08-0204） National Natural Science Foundation of China（20976060） the Scientific Research Foundation for the Returned Overseas Chinese Scholars State Edu-cation Ministry （China）
文摘A novel polymer/SiO2 hybrid emulsion(PAES)was prepared by directly mixing colloidal silica with polyacrylate emulsion(PAE)modified by a saline coupling agent.The sol-gel-derived thin films were obtained by addition of co-solvents into the PAES.The effects ofγ-methacryloxypropyltrimethoxysilane(KH-570)content and co-solvent on the properties of PAES films were investigated.Dynamic laser scattering(DLS)data indicate that the average diameter of PAES(96 nm)is slightly larger than that of PAE(89 nm).Transmission electron microscopy (TEM)photo discloses that colloidal silica particles are dispersed uniformly around polyacrylate particles and some of the colloidal silica particles are adsorbed on the surface of PAE particles.The crosslinking degree data and Fourier transform infrared(FT-IR)spectra confirm that the chemical structure of the PAES is changed to form Si-O-Si-polymer crosslinking networks during the film formation.Atomic force microscope(AFM)photos show the solvent induced sol-gel process of colloidal silica and the Si-based polymer distribution on the film surface of the dried PAES.Thermogravimetric analysis(TGA)curves demonstrate that the PAES films display much better thermal stability than PAE.
