MRFs(Myogenic regulatory factors,MRFs)家族包括肌分化因子Myo D、肌细胞生成素(myogenin)、生肌决定因子Myf5和Myf6,它们参与肌肉发生发育的各个环节。研究采用Real-time PCR方法研究了金定鸭胚胎期13、17、21、25和27日胚龄MRFs家...MRFs(Myogenic regulatory factors,MRFs)家族包括肌分化因子Myo D、肌细胞生成素(myogenin)、生肌决定因子Myf5和Myf6,它们参与肌肉发生发育的各个环节。研究采用Real-time PCR方法研究了金定鸭胚胎期13、17、21、25和27日胚龄MRFs家族基因在骨骼肌中的表达变化,并分析其表达变化与胚重、骨骼肌发育的相关性。金定鸭胚重及骨骼肌重发育模式显示胚重在胚胎期呈持续增加趋势,但胸肌在胚胎中后期显示出较腿肌滞后的增重变化。MRFs家族基因在胚胎期骨骼肌中均能检测到表达,Myo D m RNA在21日胚龄前腿肌中的表达量高于胸肌,而在胚胎发育后期(25及27日胚龄)胸肌中表达量渐渐高于腿肌。胸、腿肌中myogenin m RNA的表达与胚重、胸、腿肌发育重量变化均呈强的负线性相关。上述研究结果首次揭示了鸭胚胎期骨骼肌中MRFs家族基因表达具有显著的时空性,推测Myo D m RNA在胸、腿肌中的表达变化可能与胸、腿肌增重表型差异有关,研究结果为进一步深入研究MRFs家族基因在胚胎期骨骼肌发生过程及其调控机理中的功能提供一定的理论依据。展开更多
[Objective] Aim to know the whole process of embryonic development of loach. [Method] DOM + LHRH-A2 was used to induce spawning of loach,then after fertilization,the embryos were cultured into freshwater water with te...[Objective] Aim to know the whole process of embryonic development of loach. [Method] DOM + LHRH-A2 was used to induce spawning of loach,then after fertilization,the embryos were cultured into freshwater water with temperature from 24 to 26 ℃ and pH from 7.0 to 7.5. The embryonic development of loach was observed and 27 concrete morphological characteristics and development time of loach from fertilized egg to newly hatched larval period were described in detail. [Result] The embryonic development of loach could be divided into cleavage stage,blastocyst stage,gastrula stage,neurula stage and organogenesis stage. The loach embryo from fertilized egg to out membrane period was 30 h 45 min in fresh water from 24 to 26 ℃ and pH from 7.0 to 7.5. [Conclusion] It provided important reference for studying artificial propagation and genetic breeding of loach.展开更多
文摘MRFs(Myogenic regulatory factors,MRFs)家族包括肌分化因子Myo D、肌细胞生成素(myogenin)、生肌决定因子Myf5和Myf6,它们参与肌肉发生发育的各个环节。研究采用Real-time PCR方法研究了金定鸭胚胎期13、17、21、25和27日胚龄MRFs家族基因在骨骼肌中的表达变化,并分析其表达变化与胚重、骨骼肌发育的相关性。金定鸭胚重及骨骼肌重发育模式显示胚重在胚胎期呈持续增加趋势,但胸肌在胚胎中后期显示出较腿肌滞后的增重变化。MRFs家族基因在胚胎期骨骼肌中均能检测到表达,Myo D m RNA在21日胚龄前腿肌中的表达量高于胸肌,而在胚胎发育后期(25及27日胚龄)胸肌中表达量渐渐高于腿肌。胸、腿肌中myogenin m RNA的表达与胚重、胸、腿肌发育重量变化均呈强的负线性相关。上述研究结果首次揭示了鸭胚胎期骨骼肌中MRFs家族基因表达具有显著的时空性,推测Myo D m RNA在胸、腿肌中的表达变化可能与胸、腿肌增重表型差异有关,研究结果为进一步深入研究MRFs家族基因在胚胎期骨骼肌发生过程及其调控机理中的功能提供一定的理论依据。
文摘[Objective] Aim to know the whole process of embryonic development of loach. [Method] DOM + LHRH-A2 was used to induce spawning of loach,then after fertilization,the embryos were cultured into freshwater water with temperature from 24 to 26 ℃ and pH from 7.0 to 7.5. The embryonic development of loach was observed and 27 concrete morphological characteristics and development time of loach from fertilized egg to newly hatched larval period were described in detail. [Result] The embryonic development of loach could be divided into cleavage stage,blastocyst stage,gastrula stage,neurula stage and organogenesis stage. The loach embryo from fertilized egg to out membrane period was 30 h 45 min in fresh water from 24 to 26 ℃ and pH from 7.0 to 7.5. [Conclusion] It provided important reference for studying artificial propagation and genetic breeding of loach.
