Humic acid(HA) is a readily available and low-cost material that is used to enhance crop production and reduce nitrogen(N) loss. However, there is little consensus on the efficacy of different HA components. In the cu...Humic acid(HA) is a readily available and low-cost material that is used to enhance crop production and reduce nitrogen(N) loss. However, there is little consensus on the efficacy of different HA components. In the current study, a soil column experiment was conducted using the ^(15)N tracer technique in Dezhou City, Shandong Province, China, to compare the effects of urea with and without the addition of weathered coal-derived HA components on maize yield and the fate of fertilizerderived N(fertilizer N). The HA components were incorporated into urea by blending different HA components into molten urea to obtain the three different types of HA-enhanced urea(HAU). At harvest, the aboveground dry biomass of plants grown with HAU was enhanced by 11.50–21.33% when compared to that of plants grown with U. More significantly, the grain yields under the HAU treatments were 5.58–18.67% higher than the yield under the urea treatment. These higher yields were due to an increase in the number of kernels per plant rather than the weight of individual kernels. The uptake of fertilizer N under the HAU treatments was also higher than that under the urea treatment by 11.49–29.46%, while the unaccounted N loss decreased by 12.37–30.05%. More fertilizer-derived N was retained in the 0–30 cm soil layer under the HAU treatments than that under the urea treatment, while less N was retained in the 30–90 cm soil layer. The total residual amount of fertilizer N in the soil column, however, did not differ significantly between the treatments. Of the three HAU treatments investigated, the one with an HA fraction derived from extraction with pH values ranging from 6 to 7, resulted in the best improvement in all assessment targets. This is likely due to the abundance of the COO/C–N=O group in this HA component.展开更多
Temporally irregular rainfall distribution and inefficient rainwater management create severe constraints on crop production in rainfed semiarid areas. Gravel and plastic film mulching are effective methods for improv...Temporally irregular rainfall distribution and inefficient rainwater management create severe constraints on crop production in rainfed semiarid areas. Gravel and plastic film mulching are effective methods for improving agricultural productivity and water utilization. However, the effects of these mulching practices on soil water supply and plant water use associated with crop yield are not well understood. A 3-yr study was conducted to analyze the occurrence and distribution of dry spells in a semiarid region of Northwest China and to evaluate the effects of non-mulching （CK）, gravel mulching （GM） and plastic film mulching （FM） on the soil water supply, plant water use and maize （Zea mays L.） grain yield. Rainfall analysis showed that dry spells of ≥5 days occurred frequently in each of 3 yr, accounting for 59.9-69.2% of the maize growing periods. The 〉15-d dry spells during the jointing stage would expose maize plants to particularly severe water stress. Compared with the CK treatment, both the GM and FM treatments markedly increased soil water storage during the early growing season. In general, the total evapotranspiration （ET） was not significantly different among the three treatments, but the mulched treatments significantly increased the ratio of pre- to post-silking ET, which was closely associated with yield improvement. As a result, the grain yield significantly increased by 17.1, 70.3 and 16.7% for the GM treatment and by 28.3, 87.6 and 38.2% for the FM treatment in 2010, 2011 and 2012, respectively, compared with the CK treatment. It＇s concluded that both GM and FM are effective strategies for mitigating the impacts of water deficit and improving maize production in semiarid areas. However. FM is more effective than GM.展开更多
Over-use of N fertilizer in crop production has resulted in a series of environmental problems in the North China Plain(NCP).Thus,improvement of nitrogen use efficiency(NUE)in summer maize has become an effective stra...Over-use of N fertilizer in crop production has resulted in a series of environmental problems in the North China Plain(NCP).Thus,improvement of nitrogen use efficiency(NUE)in summer maize has become an effective strategy for promoting sustainable agriculture in this region.Using twenty maize cultivars,plant dry matter production,N absorption and accumulation,yield formation,and NUE in summer maize were investigated under three N levels in two growing seasons.Based on their yield and yield components,these maize cultivars were categorized into four groups including efficient-efficient(EE)cultivars,high-nitrogen efficient(HNE)cultivars,low-nitrogen efficient(LNE)cultivars and nonefficient-nonefficient(NN)cultivars.In both two seasons,the EE cultivars improved grain yield together with increased plant biomass,and enhanced accumulative amounts as well as higher average grain yields than the other cultivar groups under deficient-N conditions.Significant correlations were observed between yield and kernel numbers(KN),dry matter(DM)amount and N accumulation at both post-silking and maturity stages.DM and N accumulation at late growth stage(i.e.,from silking to maturity)contributed largely to the enhanced yield capacity and improved NUE under N-deficient conditions.Compared with the NN cultivars,the EE cultivars also showed increased N assimilation amount(NAA)and N remobilization content(NRC),and elevated N remobilization efficiency(NRE),NUE and nitrogen partial factor productivity(PFPN).Our investigation has revealed N-associated physiological processes and may provide guidance for cultivation and breeding of high yield and NUE summer maize under limited N conditions in the NCP.展开更多
Post-silking high temperature is one of the abiotic factors that affects waxy maize(Zea mays L. sinensis Kulesh) growth in southern China. We conducted a pot trial in 2016–2017 to study the effects of post-silking da...Post-silking high temperature is one of the abiotic factors that affects waxy maize(Zea mays L. sinensis Kulesh) growth in southern China. We conducted a pot trial in 2016–2017 to study the effects of post-silking daytime heat stress(35°C) on the activities of enzymes involved in leaf carbon and nitrogen metabolisms and leaf reactive oxygen species(ROS) and water contents. This study could improve our understanding on dry matter accumulation and translocation and grain yield production. Results indicated that decreased grain number and weight under heat stress led to yield loss, which decreased by 20.8 and 20.0% in 2016 and 2017, respectively. High temperature reduced post-silking dry matter accumulation(16.1 and 29.5% in 2016 and 2017, respectively) and promoted translocation of pre-silking photoassimilates stored in vegetative organs, especially in leaf. The lower leaf water content and chlorophyll SPAD value, and higher ROS(H2O2 and O2^-·) content under heat stress conditions indicated accelerated senescent rate. The weak activities of phosphoenolpyruvate carboxylase(PEPCase), Ribulose-1,5-bisphosphate carboxylase(Ru BPCase), nitrate reductase(NR), and glutamine synthase(GS) indicated that leaf carbon and nitrogen metabolisms were suppressed when the plants suffered from a high temperature during grain filling. Correlation analysis results indicated that the reduced grain yield was mainly caused by the decreased leaf water content, weakened NR activity, and increased H2O2 content. The increased accumulation of grain weight and post-silking dry matter and the reduced translocation amount in leaf was mainly due to the increased chlorophyll SPAD value and NR activity. Reduced PEPCase and Ru BPCase activities did not affect dry matter accumulation and translocation and grain yield. In conclusion, post-silking heat stress down-regulated the leaf NR and GS activities, increased the leafwater loss rate, increased ROS generation, and induced pre-silking carbohydrate translocation. However, it reduced the post-silk展开更多
基金supported by the National Natural Science Foundation of China (31601827)the National Key Research and Development Program of China (2016YFD0200402)
文摘Humic acid(HA) is a readily available and low-cost material that is used to enhance crop production and reduce nitrogen(N) loss. However, there is little consensus on the efficacy of different HA components. In the current study, a soil column experiment was conducted using the ^(15)N tracer technique in Dezhou City, Shandong Province, China, to compare the effects of urea with and without the addition of weathered coal-derived HA components on maize yield and the fate of fertilizerderived N(fertilizer N). The HA components were incorporated into urea by blending different HA components into molten urea to obtain the three different types of HA-enhanced urea(HAU). At harvest, the aboveground dry biomass of plants grown with HAU was enhanced by 11.50–21.33% when compared to that of plants grown with U. More significantly, the grain yields under the HAU treatments were 5.58–18.67% higher than the yield under the urea treatment. These higher yields were due to an increase in the number of kernels per plant rather than the weight of individual kernels. The uptake of fertilizer N under the HAU treatments was also higher than that under the urea treatment by 11.49–29.46%, while the unaccounted N loss decreased by 12.37–30.05%. More fertilizer-derived N was retained in the 0–30 cm soil layer under the HAU treatments than that under the urea treatment, while less N was retained in the 30–90 cm soil layer. The total residual amount of fertilizer N in the soil column, however, did not differ significantly between the treatments. Of the three HAU treatments investigated, the one with an HA fraction derived from extraction with pH values ranging from 6 to 7, resulted in the best improvement in all assessment targets. This is likely due to the abundance of the COO/C–N=O group in this HA component.
基金financially supported by the National Natural Science Foundation of China (31270553)the National Basic Research Program of China (2009CB118604)the Special Fund for Agro-Scientific Research in the Public Interest of China (201103003)
文摘Temporally irregular rainfall distribution and inefficient rainwater management create severe constraints on crop production in rainfed semiarid areas. Gravel and plastic film mulching are effective methods for improving agricultural productivity and water utilization. However, the effects of these mulching practices on soil water supply and plant water use associated with crop yield are not well understood. A 3-yr study was conducted to analyze the occurrence and distribution of dry spells in a semiarid region of Northwest China and to evaluate the effects of non-mulching （CK）, gravel mulching （GM） and plastic film mulching （FM） on the soil water supply, plant water use and maize （Zea mays L.） grain yield. Rainfall analysis showed that dry spells of ≥5 days occurred frequently in each of 3 yr, accounting for 59.9-69.2% of the maize growing periods. The 〉15-d dry spells during the jointing stage would expose maize plants to particularly severe water stress. Compared with the CK treatment, both the GM and FM treatments markedly increased soil water storage during the early growing season. In general, the total evapotranspiration （ET） was not significantly different among the three treatments, but the mulched treatments significantly increased the ratio of pre- to post-silking ET, which was closely associated with yield improvement. As a result, the grain yield significantly increased by 17.1, 70.3 and 16.7% for the GM treatment and by 28.3, 87.6 and 38.2% for the FM treatment in 2010, 2011 and 2012, respectively, compared with the CK treatment. It＇s concluded that both GM and FM are effective strategies for mitigating the impacts of water deficit and improving maize production in semiarid areas. However. FM is more effective than GM.
基金supported by the National Key Research and Development Program of China (2018YFD0300503)
文摘Over-use of N fertilizer in crop production has resulted in a series of environmental problems in the North China Plain(NCP).Thus,improvement of nitrogen use efficiency(NUE)in summer maize has become an effective strategy for promoting sustainable agriculture in this region.Using twenty maize cultivars,plant dry matter production,N absorption and accumulation,yield formation,and NUE in summer maize were investigated under three N levels in two growing seasons.Based on their yield and yield components,these maize cultivars were categorized into four groups including efficient-efficient(EE)cultivars,high-nitrogen efficient(HNE)cultivars,low-nitrogen efficient(LNE)cultivars and nonefficient-nonefficient(NN)cultivars.In both two seasons,the EE cultivars improved grain yield together with increased plant biomass,and enhanced accumulative amounts as well as higher average grain yields than the other cultivar groups under deficient-N conditions.Significant correlations were observed between yield and kernel numbers(KN),dry matter(DM)amount and N accumulation at both post-silking and maturity stages.DM and N accumulation at late growth stage(i.e.,from silking to maturity)contributed largely to the enhanced yield capacity and improved NUE under N-deficient conditions.Compared with the NN cultivars,the EE cultivars also showed increased N assimilation amount(NAA)and N remobilization content(NRC),and elevated N remobilization efficiency(NRE),NUE and nitrogen partial factor productivity(PFPN).Our investigation has revealed N-associated physiological processes and may provide guidance for cultivation and breeding of high yield and NUE summer maize under limited N conditions in the NCP.
基金supported by the National Key Research and Development Program of China (2016YFD0300109 and 2018YFD0200703)the National Natural Science Foundation of China (31771709 and 31471436)the Priority Academic Program Development of Jiangsu Higher Education Institutions, China
文摘Post-silking high temperature is one of the abiotic factors that affects waxy maize(Zea mays L. sinensis Kulesh) growth in southern China. We conducted a pot trial in 2016–2017 to study the effects of post-silking daytime heat stress(35°C) on the activities of enzymes involved in leaf carbon and nitrogen metabolisms and leaf reactive oxygen species(ROS) and water contents. This study could improve our understanding on dry matter accumulation and translocation and grain yield production. Results indicated that decreased grain number and weight under heat stress led to yield loss, which decreased by 20.8 and 20.0% in 2016 and 2017, respectively. High temperature reduced post-silking dry matter accumulation(16.1 and 29.5% in 2016 and 2017, respectively) and promoted translocation of pre-silking photoassimilates stored in vegetative organs, especially in leaf. The lower leaf water content and chlorophyll SPAD value, and higher ROS(H2O2 and O2^-·) content under heat stress conditions indicated accelerated senescent rate. The weak activities of phosphoenolpyruvate carboxylase(PEPCase), Ribulose-1,5-bisphosphate carboxylase(Ru BPCase), nitrate reductase(NR), and glutamine synthase(GS) indicated that leaf carbon and nitrogen metabolisms were suppressed when the plants suffered from a high temperature during grain filling. Correlation analysis results indicated that the reduced grain yield was mainly caused by the decreased leaf water content, weakened NR activity, and increased H2O2 content. The increased accumulation of grain weight and post-silking dry matter and the reduced translocation amount in leaf was mainly due to the increased chlorophyll SPAD value and NR activity. Reduced PEPCase and Ru BPCase activities did not affect dry matter accumulation and translocation and grain yield. In conclusion, post-silking heat stress down-regulated the leaf NR and GS activities, increased the leafwater loss rate, increased ROS generation, and induced pre-silking carbohydrate translocation. However, it reduced the post-silk
