The interaction of double-stranded （ds） and G-quadruplex （G4） DNA with sulfonyl 5-fluorouracil derivatives （5-fluoro-l-（arylsulfonyl） pyrimidine-2,4 （1H,3H）-diones） was investigated in this research, in whic...The interaction of double-stranded （ds） and G-quadruplex （G4） DNA with sulfonyl 5-fluorouracil derivatives （5-fluoro-l-（arylsulfonyl） pyrimidine-2,4 （1H,3H）-diones） was investigated in this research, in which Au electrodes modified with ds-DNA or G4-DNAs were used as a working electrode. The investigation showed that the binding affinity with G4-DNA was significantly increased when 5-fluorouracil （5-FU） was modified with arylsulfonyl groups. The presence of strong electron-withdrawing groups on benzene sulfonyl 5-FU greatly enhanced the binding selectivity （kG4-DNA/kds-DNA）. Such results provided new insights into the potential connections between the chemical structure of drug candidates and their anticancer activities.展开更多
The site-specific mutagenesis of the gene has become an important technique in gene modification and protein engineering. Among all methods, the primer extension one using single-stranded DNA (such as the infective fo...The site-specific mutagenesis of the gene has become an important technique in gene modification and protein engineering. Among all methods, the primer extension one using single-stranded DNA (such as the infective form of the M13 phage ) as template and the gapped stranded one are commonly used. But some genes, especially those展开更多
Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/ sensing technologies.In this paper,we report that the fluorescence of the double-stranded DNA(dsDNA) and SYBR GreenⅠ...Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/ sensing technologies.In this paper,we report that the fluorescence of the double-stranded DNA(dsDNA) and SYBR GreenⅠ(SG) system(dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe_2O_3@DMSA) and gold nanoparticles in suitable concentrations,but positively charged nanoparticles quench the fluorescence.Effects of the Fe_2O_3@DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities.The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA.However,the dsDNA concentration determines the fluorescence amplitude.It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG.The finding may be useful in sensitive biomolecular detection.展开更多
基金supported by the National Natural Science Foundation of China (21073133,20843007)Zhejiang Provincial Natural Science Foundation of China (Y4080177,Y4090248,Y5100283)Zhejiang Provincial Ministry of Education (Y200907715)
文摘The interaction of double-stranded （ds） and G-quadruplex （G4） DNA with sulfonyl 5-fluorouracil derivatives （5-fluoro-l-（arylsulfonyl） pyrimidine-2,4 （1H,3H）-diones） was investigated in this research, in which Au electrodes modified with ds-DNA or G4-DNAs were used as a working electrode. The investigation showed that the binding affinity with G4-DNA was significantly increased when 5-fluorouracil （5-FU） was modified with arylsulfonyl groups. The presence of strong electron-withdrawing groups on benzene sulfonyl 5-FU greatly enhanced the binding selectivity （kG4-DNA/kds-DNA）. Such results provided new insights into the potential connections between the chemical structure of drug candidates and their anticancer activities.
基金Project supported by the National High Technology Foundation (Term No. 103-20).
文摘The site-specific mutagenesis of the gene has become an important technique in gene modification and protein engineering. Among all methods, the primer extension one using single-stranded DNA (such as the infective form of the M13 phage ) as template and the gapped stranded one are commonly used. But some genes, especially those
基金supported by grants from the National Science Foundation of China (No.10975175,90923002)the Chinese Academy of Sciences(No.KJCX2-EW-N03)
文摘Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/ sensing technologies.In this paper,we report that the fluorescence of the double-stranded DNA(dsDNA) and SYBR GreenⅠ(SG) system(dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe_2O_3@DMSA) and gold nanoparticles in suitable concentrations,but positively charged nanoparticles quench the fluorescence.Effects of the Fe_2O_3@DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities.The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA.However,the dsDNA concentration determines the fluorescence amplitude.It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG.The finding may be useful in sensitive biomolecular detection.
