摘要
目的研究能够用于DNA适体筛选的双链DNA拆分方法。方法采用琼脂糖凝胶电泳,观察和比较λ核酸外切酶法、不对称PCR法、磁珠法以及琼脂糖凝胶法的拆分效果。结果琼脂糖凝胶电泳显示,λ核酸外切酶法不能充分拆分双链DNA形成单链DNA;不对称PCR法产生的非特异性单链DNA较多;磁珠法拆分双链DNA获得单链DNA的量较低,且成本较高;琼脂糖凝胶法拆分双链DNA较为充分,单链DNA纯度较高。结论琼脂糖凝胶法可用于DNA适体筛选中双链DNA的拆分。
Objective Study on a split method of double-stranded DNA for selecting DNA aptamer. Methods Effects of h exonuclease, asymmetry PCR, magnetic beads, and agarose gel were observed and compared by agarose gel electrophoresis. Results Double-stranded DNA was not separated sufficiently by exonuclease. The nonspeeific single-stranded DNA amplified by asymmetry PCR was too more. The amount of the single-stranded DNA obtained by magnetic beads was low buy with high cost. The purity of single-stranded DNA obtained from double-stranded DNA separated sufficiently by agarose gel was high. Conclusions The agarose gel method can be used for the splitting of the double-stranded DNA in the DNA aptamer screening.
出处
《齐齐哈尔医学院学报》
2013年第8期1105-1107,共3页
Journal of Qiqihar Medical University
基金
佳木斯大学大学生创新创业训练计划项目(2012sj005)
