研究了超声波辅助酶解脱脂小麦胚芽对酶解产物抗氧化性的影响。在单因素实验基础上,通过响应面法考察了超声功率、超声时间和超声温度对酶解产物的1,1-二苯基-2-苦基肼(DPPH)清除率的影响。结果表明,最佳的超声酶解条件为:超声功率214 W...研究了超声波辅助酶解脱脂小麦胚芽对酶解产物抗氧化性的影响。在单因素实验基础上,通过响应面法考察了超声功率、超声时间和超声温度对酶解产物的1,1-二苯基-2-苦基肼(DPPH)清除率的影响。结果表明,最佳的超声酶解条件为:超声功率214 W,超声时间3 m in,超声温度25℃。此时酶解产物的DPPH清除率理论值为62.4%,实际的清除率达到61.2%。展开更多
Wheat germ is a by-product derived from the wheat milling industry. Defatted wheat germ is the main by-product of the wheat germ in the oil extraction process. This study aims at development of efficient and low cost ...Wheat germ is a by-product derived from the wheat milling industry. Defatted wheat germ is the main by-product of the wheat germ in the oil extraction process. This study aims at development of efficient and low cost processing methods to transform these residues in added value co-product. In this study, wheat germ was analysed for its proximate composition, fatty acid composition, physical and chemical characteristics of wheat germ oil. The basic chemical composition analyses revealed high values of dry matter (87.37 g/100g FW), significant amounts of total protein and fat (27.69 and 8.99 g/100g FW, respectively) content and low ash content (3.08 g/100g FW). The quality of the extracted oils was assessed in terms of acid value, iodine value, saponification value, peroxide value, refractive index, and unsaponifiable matter. The fatty acid profile was found to be made up of linoleic followed by palmitic and oleic as the major fatty acids. Antioxidant properties and in vitro antibacterial activity of defatted wheat germ (DWG) extract were also determined. DWG, as a source of natural antioxidants and antibacterial, could be used to formulate nutraceuticals with potential applications to reduce the level of oxidative stress. The antioxidant potency of the DWG extracts could be the basis for its health promoting potential. The results showed that these by-products could be used as a source of bioactive compounds beneficial for health.展开更多
为了研究不同工作模式超声预处理对脱脂小麦胚芽水解度和ACE抑制率的影响,在相同能耗条件,利用聚能逆流单频、聚能逆流双频、脉冲扫频多频、发散三频、对振双频五种工作模式超声,对脱脂小麦胚芽进行预处理。同时进行单因素实验来寻找最...为了研究不同工作模式超声预处理对脱脂小麦胚芽水解度和ACE抑制率的影响,在相同能耗条件,利用聚能逆流单频、聚能逆流双频、脉冲扫频多频、发散三频、对振双频五种工作模式超声,对脱脂小麦胚芽进行预处理。同时进行单因素实验来寻找最佳超声预处理参数。结果表明,超声波预处理对水解度没有显著的影响,但可以显著提高酶解产物的ACE抑制活性,最佳的超声波工作模式为脉冲平板式40 k Hz/28 k Hz双频超声;在此模式下超声预处理单位体积超声功率60 W/L、超声时间70 min、超声初始温度60℃、底物浓度7%时得到的酶解产物IC_(50)值低至2.483 mg/m L,为较优结果,和未超声相比,其产物IC_(50)值降低了5.8%。展开更多
文摘研究了超声波辅助酶解脱脂小麦胚芽对酶解产物抗氧化性的影响。在单因素实验基础上,通过响应面法考察了超声功率、超声时间和超声温度对酶解产物的1,1-二苯基-2-苦基肼(DPPH)清除率的影响。结果表明,最佳的超声酶解条件为:超声功率214 W,超声时间3 m in,超声温度25℃。此时酶解产物的DPPH清除率理论值为62.4%,实际的清除率达到61.2%。
文摘Wheat germ is a by-product derived from the wheat milling industry. Defatted wheat germ is the main by-product of the wheat germ in the oil extraction process. This study aims at development of efficient and low cost processing methods to transform these residues in added value co-product. In this study, wheat germ was analysed for its proximate composition, fatty acid composition, physical and chemical characteristics of wheat germ oil. The basic chemical composition analyses revealed high values of dry matter (87.37 g/100g FW), significant amounts of total protein and fat (27.69 and 8.99 g/100g FW, respectively) content and low ash content (3.08 g/100g FW). The quality of the extracted oils was assessed in terms of acid value, iodine value, saponification value, peroxide value, refractive index, and unsaponifiable matter. The fatty acid profile was found to be made up of linoleic followed by palmitic and oleic as the major fatty acids. Antioxidant properties and in vitro antibacterial activity of defatted wheat germ (DWG) extract were also determined. DWG, as a source of natural antioxidants and antibacterial, could be used to formulate nutraceuticals with potential applications to reduce the level of oxidative stress. The antioxidant potency of the DWG extracts could be the basis for its health promoting potential. The results showed that these by-products could be used as a source of bioactive compounds beneficial for health.
文摘为了研究不同工作模式超声预处理对脱脂小麦胚芽水解度和ACE抑制率的影响,在相同能耗条件,利用聚能逆流单频、聚能逆流双频、脉冲扫频多频、发散三频、对振双频五种工作模式超声,对脱脂小麦胚芽进行预处理。同时进行单因素实验来寻找最佳超声预处理参数。结果表明,超声波预处理对水解度没有显著的影响,但可以显著提高酶解产物的ACE抑制活性,最佳的超声波工作模式为脉冲平板式40 k Hz/28 k Hz双频超声;在此模式下超声预处理单位体积超声功率60 W/L、超声时间70 min、超声初始温度60℃、底物浓度7%时得到的酶解产物IC_(50)值低至2.483 mg/m L,为较优结果,和未超声相比,其产物IC_(50)值降低了5.8%。
