A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus indu...A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus induction; MS medium containing 6\|BA 0.3 mg/L and IAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IAA 0.5 mg/L and ABT 0.1 mg/L is effective in inducing root with the ratio of 95%, fast propagation of P. grandiflorum by tissue culture method could be established, which will lay down the foundation of the breeding and clone of excellent varieties of P. grandiflorum.展开更多
文摘A Study of tissue culture in vitro and plant reproduction of Platycodon grandiflorum A. DC. was carried out. The results indicated that MS medium containing 6\|BA 1.0 mg/L and IAA 0.5 mg/L was the best for callus induction; MS medium containing 6\|BA 0.3 mg/L and IAA 0.1 mg/L was suitable for crowd bud propagation; 1/2 MS medium containing IAA 0.5 mg/L and ABT 0.1 mg/L is effective in inducing root with the ratio of 95%, fast propagation of P. grandiflorum by tissue culture method could be established, which will lay down the foundation of the breeding and clone of excellent varieties of P. grandiflorum.