Neurotrophin family are traditionally recognized for their nerve growth promoting function and are recently identified as crucial factors in regulating neuronal activity in the central and peripheral nervous systems. ...Neurotrophin family are traditionally recognized for their nerve growth promoting function and are recently identified as crucial factors in regulating neuronal activity in the central and peripheral nervous systems. The family members including nerve growth factor （NGF）, brain-derived neurotrophic factor （BDNF）, and neurotrophin-3 （NT-3） are reported to have distinct roles in the development and maintenance of sensory phenotypes in normal states and in the modulation of sensory activity in disease. This paper highlights receptor tyrosine kinase （Trk） -mediated signal transduction by which neurotrophins regulate neuronal activity in the visceral sensory reflex pathways with emphasis on the distinct roles of NGF and BDNF signaling in physiologic and pathophysiological processes. Viscero-visceral cross-organ sensitization exists widely in human diseases. The role of neurotrophins in mediating neural cross talk and interaction in primary afferent neurons in the dorsal root ganglia （DRG） and neurotrophin signal transduction in the context of cross-organ sensitization are also discussed.展开更多
目的:探讨慢性前列腺炎性刺激对膀胱功能的影响。方法:前列腺内注射完全弗氏佐剂(Completion of freund adjuvant,CFA)制作大鼠慢性前列腺炎症模型,设立生理盐水对照组和假手术组,分别于前列腺注射前后在清醒状态下行尿动力学检测。结果...目的:探讨慢性前列腺炎性刺激对膀胱功能的影响。方法:前列腺内注射完全弗氏佐剂(Completion of freund adjuvant,CFA)制作大鼠慢性前列腺炎症模型,设立生理盐水对照组和假手术组,分别于前列腺注射前后在清醒状态下行尿动力学检测。结果:CFA实验组前列腺炎性刺激后,充盈性膀胱压力测定显示膀胱基础压和排尿前压增加(P<0.05),膀胱容量降低(P<0.05),最大排尿压降低(P<0.01)。对照组和假手术组尿动力学参数前后比较无统计学意义(P>0.05)。结论:前列腺炎性刺激能引起膀胱跨器官痛觉致敏,与慢性前列腺炎/慢性盆底痛综合征的排尿功能异常有关。展开更多
背景:内脏-内脏之间存在广泛的伤害性传入会聚和相互作用,在跨器官痛觉过敏的发生中起重要作用。目的:在内脏高敏感状态下建立跨器官敏化大鼠模型,探讨蛋白激酶Cγ(PKCγ)对跨器官内脏敏感性的调控作用。方法:Sprague-Dawley大鼠随机分...背景:内脏-内脏之间存在广泛的伤害性传入会聚和相互作用,在跨器官痛觉过敏的发生中起重要作用。目的:在内脏高敏感状态下建立跨器官敏化大鼠模型,探讨蛋白激酶Cγ(PKCγ)对跨器官内脏敏感性的调控作用。方法:Sprague-Dawley大鼠随机分为4组,其中3组在鸡卵清蛋白(OVA)基础致敏联合结直肠芥子油(MO)灌注建立结直肠-膀胱跨器官敏化模型的基础上,分别鞘内注射PKCγ特异性抑制剂GF109203X、0.9%NaCl溶液(NS)或不予鞘内注射,另一组为正常对照组。各组大鼠行梯度结直肠扩张/膀胱扩张(CRD/UBD),根据腹壁肌电活动曲线下面积(AUC)评估内脏敏感性的变化。结果:各组UBD梯度扩张刺激体积均与腹壁肌电活动AUC显著相关(P<0.05)。UBD扩张体积为1.0、1.5、2.0 ml以及CRD扩张压力为40、60、80 mm Hg时,OVA+MO组AUC显著高于正常对照组(P<0.05);UBD扩张体积为1.5、2.0 ml以及CRD扩张压力为60、80 mm Hg时,OVA+MO+GF109203X组AUC较OVA+MO+NS组显著降低(P<0.05)。结论:鞘内注射PKCγ特异性抑制剂能逆转跨器官交叉内脏高敏感性,提示PKCγ在跨器官敏化的产生和维持中起重要作用。展开更多
文摘Neurotrophin family are traditionally recognized for their nerve growth promoting function and are recently identified as crucial factors in regulating neuronal activity in the central and peripheral nervous systems. The family members including nerve growth factor （NGF）, brain-derived neurotrophic factor （BDNF）, and neurotrophin-3 （NT-3） are reported to have distinct roles in the development and maintenance of sensory phenotypes in normal states and in the modulation of sensory activity in disease. This paper highlights receptor tyrosine kinase （Trk） -mediated signal transduction by which neurotrophins regulate neuronal activity in the visceral sensory reflex pathways with emphasis on the distinct roles of NGF and BDNF signaling in physiologic and pathophysiological processes. Viscero-visceral cross-organ sensitization exists widely in human diseases. The role of neurotrophins in mediating neural cross talk and interaction in primary afferent neurons in the dorsal root ganglia （DRG） and neurotrophin signal transduction in the context of cross-organ sensitization are also discussed.
文摘目的:探讨慢性前列腺炎性刺激对膀胱功能的影响。方法:前列腺内注射完全弗氏佐剂(Completion of freund adjuvant,CFA)制作大鼠慢性前列腺炎症模型,设立生理盐水对照组和假手术组,分别于前列腺注射前后在清醒状态下行尿动力学检测。结果:CFA实验组前列腺炎性刺激后,充盈性膀胱压力测定显示膀胱基础压和排尿前压增加(P<0.05),膀胱容量降低(P<0.05),最大排尿压降低(P<0.01)。对照组和假手术组尿动力学参数前后比较无统计学意义(P>0.05)。结论:前列腺炎性刺激能引起膀胱跨器官痛觉致敏,与慢性前列腺炎/慢性盆底痛综合征的排尿功能异常有关。
文摘背景:内脏-内脏之间存在广泛的伤害性传入会聚和相互作用,在跨器官痛觉过敏的发生中起重要作用。目的:在内脏高敏感状态下建立跨器官敏化大鼠模型,探讨蛋白激酶Cγ(PKCγ)对跨器官内脏敏感性的调控作用。方法:Sprague-Dawley大鼠随机分为4组,其中3组在鸡卵清蛋白(OVA)基础致敏联合结直肠芥子油(MO)灌注建立结直肠-膀胱跨器官敏化模型的基础上,分别鞘内注射PKCγ特异性抑制剂GF109203X、0.9%NaCl溶液(NS)或不予鞘内注射,另一组为正常对照组。各组大鼠行梯度结直肠扩张/膀胱扩张(CRD/UBD),根据腹壁肌电活动曲线下面积(AUC)评估内脏敏感性的变化。结果:各组UBD梯度扩张刺激体积均与腹壁肌电活动AUC显著相关(P<0.05)。UBD扩张体积为1.0、1.5、2.0 ml以及CRD扩张压力为40、60、80 mm Hg时,OVA+MO组AUC显著高于正常对照组(P<0.05);UBD扩张体积为1.5、2.0 ml以及CRD扩张压力为60、80 mm Hg时,OVA+MO+GF109203X组AUC较OVA+MO+NS组显著降低(P<0.05)。结论:鞘内注射PKCγ特异性抑制剂能逆转跨器官交叉内脏高敏感性,提示PKCγ在跨器官敏化的产生和维持中起重要作用。
