为探索干制条件对红枣香气品质的影响,采用固相微萃取结合气相色谱/质谱联用(SPME-GC/MS,solid phase microextraction coupled with gas chromatography mass pectrometry)技术分析鉴定了50、60、70℃热风干燥,自然阴干和糖心枣5种红...为探索干制条件对红枣香气品质的影响,采用固相微萃取结合气相色谱/质谱联用(SPME-GC/MS,solid phase microextraction coupled with gas chromatography mass pectrometry)技术分析鉴定了50、60、70℃热风干燥,自然阴干和糖心枣5种红枣样品的香气成分及相对质量分数,对红枣中7类主要香气物质进行主成分分析(principal component analysis,PCA)。结果表明影响红枣香气品质的主要香气物质种类为酯类、醛类、酸类和酚类;自然阴干的红枣香气品质较差,采用热风干制可有效提高红枣的香气品质;60℃热风干制的红枣香气成分综合得分最高,优于50℃和70℃热风干制红枣的香气品质,为红枣的干制工艺提供技术依据。主成分分析法可实现对红枣干制条件的区分,并能较好评价红枣的香气品质。展开更多
Due to the scarcity of resources of Ziziphi spinosae semen （ZSS）, many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern ...Due to the scarcity of resources of Ziziphi spinosae semen （ZSS）, many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern established in this paper showed three main bioactive compounds in one chromatogram simultaneously. Principal component analysis based on DAD signals could discriminate adulterants and inferiorities. Principal component analysis indicated that all samples could be mainly regrouped into two main clusters according to the first principal component （PC1, redefined as Vicenin II） and the second principal component （PC2, redefined as zizyphusine）. PC1 and PC2 could explain 91.42%of the variance. Content of zizyphusine fluctuated more greatly than that of spinosin, and this result was also confirmed by the HPTLC result. Samples with low content of jujubosides and two common adulterants could not be used equivalently with authenticated ones in clinic, while one reference standard extract could substitute the crude drug in pharmaceutical production. Giving special consideration to the well-known bioactive saponins but with low response by end absorption, a fast and cheap HPTLC method for quality control of ZSS was developed and the result obtained was commensurate well with that of HPLC analysis. Samples having similar fingerprints to HPTLC common pattern targeting at saponins could be regarded as authenticated ones. This work provided a faster and cheaper way for quality control of ZSS and laid foundation for establishing a more effective quality control method for ZSS.展开更多
文摘为探索干制条件对红枣香气品质的影响,采用固相微萃取结合气相色谱/质谱联用(SPME-GC/MS,solid phase microextraction coupled with gas chromatography mass pectrometry)技术分析鉴定了50、60、70℃热风干燥,自然阴干和糖心枣5种红枣样品的香气成分及相对质量分数,对红枣中7类主要香气物质进行主成分分析(principal component analysis,PCA)。结果表明影响红枣香气品质的主要香气物质种类为酯类、醛类、酸类和酚类;自然阴干的红枣香气品质较差,采用热风干制可有效提高红枣的香气品质;60℃热风干制的红枣香气成分综合得分最高,优于50℃和70℃热风干制红枣的香气品质,为红枣的干制工艺提供技术依据。主成分分析法可实现对红枣干制条件的区分,并能较好评价红枣的香气品质。
文摘Due to the scarcity of resources of Ziziphi spinosae semen （ZSS）, many inferior goods and even adulterants are generally found in medicine markets. To strengthen the quality control, HPLC fingerprint common pattern established in this paper showed three main bioactive compounds in one chromatogram simultaneously. Principal component analysis based on DAD signals could discriminate adulterants and inferiorities. Principal component analysis indicated that all samples could be mainly regrouped into two main clusters according to the first principal component （PC1, redefined as Vicenin II） and the second principal component （PC2, redefined as zizyphusine）. PC1 and PC2 could explain 91.42%of the variance. Content of zizyphusine fluctuated more greatly than that of spinosin, and this result was also confirmed by the HPTLC result. Samples with low content of jujubosides and two common adulterants could not be used equivalently with authenticated ones in clinic, while one reference standard extract could substitute the crude drug in pharmaceutical production. Giving special consideration to the well-known bioactive saponins but with low response by end absorption, a fast and cheap HPTLC method for quality control of ZSS was developed and the result obtained was commensurate well with that of HPLC analysis. Samples having similar fingerprints to HPTLC common pattern targeting at saponins could be regarded as authenticated ones. This work provided a faster and cheaper way for quality control of ZSS and laid foundation for establishing a more effective quality control method for ZSS.
