Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isola...Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isolated 24 microsatellite markers from P.chungensis using Illumina Mi Seq sequencing.Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet.All loci were polymorphic with the number of alleles per locus ranging from 2 to 4.The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708,respectively.The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.展开更多
Aims Distyly is one of the most widespread floral polymorphisms pro-moting cross-fertilization.Evolutionary transition from obligate cross-fertilized distyly to predominantly self-fertilized homostyly is frequently do...Aims Distyly is one of the most widespread floral polymorphisms pro-moting cross-fertilization.Evolutionary transition from obligate cross-fertilized distyly to predominantly self-fertilized homostyly is frequently documented in various groups.However,empirical stud-ies concerning the ecological factors connected with this transition are still lacking.Primula chungensis,suggested to be evolving from distyly to homostyly,provides an ideal model for the study of the ecological factors concerned with this transition.We study P.chun-gensis to understand if autonomous self-fertilization would provide reproductive assurance for the self-fertilized homo-styled morph in the field.Methods the incompatibility features of P.chungensis were tested with hand-pollination experiments.We compared the capacity of autonomous self-fertilization between the distylous and homo-styled morph of P.chungensis in the field by excluding the pollinators with bags.In addition,the degrees of herkogamy of some P.chungensis plants were between the short-styled and homo-styled morphs.these plants were studied to understand whether they were able to obtain greater reproductive assurance when the herkogamy in the flowers was reduced.Important Findings All three morphs of P.chungensis were highly self-and intra-morph compatible.the degree of herkogamy positively correlated with the capacity for autonomous self-fertilization.A negative correlation between the degree of herkogamy and the magnitude of pollen limitation was found,but no significant correlation was found between the degree of herkogamy and the contribution of cross-fertilization to overall fertilization.this study suggests that reducing the degree of herkogamy can significantly increase the reproductive assurance for a self-compatible plant.Our results provided evidence that the homo-styled morph of P.chungensis had the highest capacity for autonomous self-fertilization and the highest seed production in the field,because autonomous self-fertilization provided reproduct-ive assurance fo展开更多
基金funded by the National Key Basic Research Program of China(2014CB954100)the Key Research Program of the Chinese Academic of Sciences(KJZD-EW-L07)+1 种基金the National Natural Science Foundation of China(31200289,31570384)the Natural Science Foundation of Yunnan Province(2012FB182)
文摘Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isolated 24 microsatellite markers from P.chungensis using Illumina Mi Seq sequencing.Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet.All loci were polymorphic with the number of alleles per locus ranging from 2 to 4.The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708,respectively.The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.
基金This research was supported by the Joint Funds of the National Science Foundation of China and Yunnan Provincial Government(no.U1202261)the National Natural Science Foundation of China(no.31500194)and the CAS 135 program(XTBG-F01,T01).
文摘Aims Distyly is one of the most widespread floral polymorphisms pro-moting cross-fertilization.Evolutionary transition from obligate cross-fertilized distyly to predominantly self-fertilized homostyly is frequently documented in various groups.However,empirical stud-ies concerning the ecological factors connected with this transition are still lacking.Primula chungensis,suggested to be evolving from distyly to homostyly,provides an ideal model for the study of the ecological factors concerned with this transition.We study P.chun-gensis to understand if autonomous self-fertilization would provide reproductive assurance for the self-fertilized homo-styled morph in the field.Methods the incompatibility features of P.chungensis were tested with hand-pollination experiments.We compared the capacity of autonomous self-fertilization between the distylous and homo-styled morph of P.chungensis in the field by excluding the pollinators with bags.In addition,the degrees of herkogamy of some P.chungensis plants were between the short-styled and homo-styled morphs.these plants were studied to understand whether they were able to obtain greater reproductive assurance when the herkogamy in the flowers was reduced.Important Findings All three morphs of P.chungensis were highly self-and intra-morph compatible.the degree of herkogamy positively correlated with the capacity for autonomous self-fertilization.A negative correlation between the degree of herkogamy and the magnitude of pollen limitation was found,but no significant correlation was found between the degree of herkogamy and the contribution of cross-fertilization to overall fertilization.this study suggests that reducing the degree of herkogamy can significantly increase the reproductive assurance for a self-compatible plant.Our results provided evidence that the homo-styled morph of P.chungensis had the highest capacity for autonomous self-fertilization and the highest seed production in the field,because autonomous self-fertilization provided reproduct-ive assurance fo
