目的:观察热敏化腧穴埋线与舒利迭对慢性持续期支气管哮喘的疗效差异。方法:将60例符合纳入标准的患者按照随机数字表分为治疗组(热敏化腧穴埋线法)和对照组(舒利迭吸入剂,各30例)。热敏化腧穴埋线,每周治疗1次;舒利迭吸入剂,每次1吸,...目的:观察热敏化腧穴埋线与舒利迭对慢性持续期支气管哮喘的疗效差异。方法:将60例符合纳入标准的患者按照随机数字表分为治疗组(热敏化腧穴埋线法)和对照组(舒利迭吸入剂,各30例)。热敏化腧穴埋线,每周治疗1次;舒利迭吸入剂,每次1吸,每日两次。分别在治疗3个月后及治疗后3个月对两组的肺功能(FEV1、PEF)、哮喘控制问卷(Asthma Control Test,ACT)进行分析比较,进行临床疗效统计。结果:治疗3个月结束时,两组肺功能、ACT评分较前明显提高(均P<0.01),组间比较差异无统计学意义(P>0.05),表明两组疗法的近期疗效基本一致;治疗结束后3个月,热敏化腧穴埋线组肺功能、ACT评分维持治疗结束时水平,而舒利迭组下降明显,且热敏化腧穴埋线组总有效率为83.3%(25/30)明显优于舒利迭组的56.7%(17/30)(P<0.05),表明热敏化腧穴埋线法疗效更持久,远期疗效更佳。结论:热敏化腧穴埋线法能有效改善慢性持续期支气管哮喘患者肺功能及临床症状,其近期疗效与舒利迭基本一致,但远期疗效优于舒利迭。展开更多
Recent discovery of occult hepatitis C virus (HCV) infection persisting after spontaneous or antiviral therapy-induced resolution of hepatitis C was made possible by the introduction of nucleic acid amplification assa...Recent discovery of occult hepatitis C virus (HCV) infection persisting after spontaneous or antiviral therapy-induced resolution of hepatitis C was made possible by the introduction of nucleic acid amplification assays capable of detecting HCV RNA at sensitivities superseding those offered by clinical tests. Although individuals with this seemingly silent HCV infection are usually anti-HCV antibody reactive and have normal liver function tests, occult HCV infection has also been reported in anti-HCV-negative individuals with persistently elevated liver enzymes of unknown etiology. Studies have shown that HCV RNA can persist for years in serum, lymphomononuclear cells and liver in the absence of clinical symptoms, although histological evidence of a mild inflammatory liver injury can be occasionally encountered. Furthermore, while HCV RNA can be detected in circulating lymphoid cells in approximately 30% of cases, a short-term culture under stimulatory conditions augments HCV replication in these cells allowing detection of virus in otherwise HCV-negative cases. HCV infects different immune cell subsets, including CD4+ and CD8+ T lymphocytes, B cells and monocytes. Studies employing clonal sequencing and single-stranded conformational polymorphism analyses have revealed unique HCV variants residing in immune cells, further strengthening the notion of HCV lymphotropism. Overall, the data accumulated suggest that occult HCV infection is a common consequence of resolution of symptomatic hepatitis C and that examination of the cells of the immune system is an effective approach to diagnosis of HCV infection and its long-term persistence. Further work is required to fully realize pathogenic and epidemiological consequences of occult HCV persistence.展开更多
目的:检测慢性HBV感染产妇胎儿脐带血单核细胞PD-L1表达情况,探讨脐血清HBeAg与PD-L1表达的关系.方法:采用流式细胞仪技术检测31例HBsAg阳性产妇和10例健康产妇胎儿脐带血单核细胞PD-L1的表达,微粒子免疫荧光法(MEIA)检测脐血清HBeAg.结...目的:检测慢性HBV感染产妇胎儿脐带血单核细胞PD-L1表达情况,探讨脐血清HBeAg与PD-L1表达的关系.方法:采用流式细胞仪技术检测31例HBsAg阳性产妇和10例健康产妇胎儿脐带血单核细胞PD-L1的表达,微粒子免疫荧光法(MEIA)检测脐血清HBeAg.结果:HBeAg阴性产妇组胎儿脐带血单核细胞PD-L1表达与健康产妇组无显著差异,而HBeAg阳性组中,3例脐血清HBeAg高水平患者,脐带血单核细胞PD-L1表达显著高于HBeAg低水平组、HBeAg阴性组及健康产妇组(22.0±1.6 vs 2.55±3.1,1.3±1.2,1.5±0.8,均P=0.00);HBeAg量处于低水平者,其单核细胞PD-L1表达与HBeAg阴性者及健康产妇组比较无显著差异.结论:慢性HBV感染产妇胎儿脐血高水平HBeAg可能和单核细胞PD-L1高表达有关,可能和新生儿HBV感染慢性化机制有关.展开更多
文摘目的:观察热敏化腧穴埋线与舒利迭对慢性持续期支气管哮喘的疗效差异。方法:将60例符合纳入标准的患者按照随机数字表分为治疗组(热敏化腧穴埋线法)和对照组(舒利迭吸入剂,各30例)。热敏化腧穴埋线,每周治疗1次;舒利迭吸入剂,每次1吸,每日两次。分别在治疗3个月后及治疗后3个月对两组的肺功能(FEV1、PEF)、哮喘控制问卷(Asthma Control Test,ACT)进行分析比较,进行临床疗效统计。结果:治疗3个月结束时,两组肺功能、ACT评分较前明显提高(均P<0.01),组间比较差异无统计学意义(P>0.05),表明两组疗法的近期疗效基本一致;治疗结束后3个月,热敏化腧穴埋线组肺功能、ACT评分维持治疗结束时水平,而舒利迭组下降明显,且热敏化腧穴埋线组总有效率为83.3%(25/30)明显优于舒利迭组的56.7%(17/30)(P<0.05),表明热敏化腧穴埋线法疗效更持久,远期疗效更佳。结论:热敏化腧穴埋线法能有效改善慢性持续期支气管哮喘患者肺功能及临床症状,其近期疗效与舒利迭基本一致,但远期疗效优于舒利迭。
文摘Recent discovery of occult hepatitis C virus (HCV) infection persisting after spontaneous or antiviral therapy-induced resolution of hepatitis C was made possible by the introduction of nucleic acid amplification assays capable of detecting HCV RNA at sensitivities superseding those offered by clinical tests. Although individuals with this seemingly silent HCV infection are usually anti-HCV antibody reactive and have normal liver function tests, occult HCV infection has also been reported in anti-HCV-negative individuals with persistently elevated liver enzymes of unknown etiology. Studies have shown that HCV RNA can persist for years in serum, lymphomononuclear cells and liver in the absence of clinical symptoms, although histological evidence of a mild inflammatory liver injury can be occasionally encountered. Furthermore, while HCV RNA can be detected in circulating lymphoid cells in approximately 30% of cases, a short-term culture under stimulatory conditions augments HCV replication in these cells allowing detection of virus in otherwise HCV-negative cases. HCV infects different immune cell subsets, including CD4+ and CD8+ T lymphocytes, B cells and monocytes. Studies employing clonal sequencing and single-stranded conformational polymorphism analyses have revealed unique HCV variants residing in immune cells, further strengthening the notion of HCV lymphotropism. Overall, the data accumulated suggest that occult HCV infection is a common consequence of resolution of symptomatic hepatitis C and that examination of the cells of the immune system is an effective approach to diagnosis of HCV infection and its long-term persistence. Further work is required to fully realize pathogenic and epidemiological consequences of occult HCV persistence.
文摘目的:检测慢性HBV感染产妇胎儿脐带血单核细胞PD-L1表达情况,探讨脐血清HBeAg与PD-L1表达的关系.方法:采用流式细胞仪技术检测31例HBsAg阳性产妇和10例健康产妇胎儿脐带血单核细胞PD-L1的表达,微粒子免疫荧光法(MEIA)检测脐血清HBeAg.结果:HBeAg阴性产妇组胎儿脐带血单核细胞PD-L1表达与健康产妇组无显著差异,而HBeAg阳性组中,3例脐血清HBeAg高水平患者,脐带血单核细胞PD-L1表达显著高于HBeAg低水平组、HBeAg阴性组及健康产妇组(22.0±1.6 vs 2.55±3.1,1.3±1.2,1.5±0.8,均P=0.00);HBeAg量处于低水平者,其单核细胞PD-L1表达与HBeAg阴性者及健康产妇组比较无显著差异.结论:慢性HBV感染产妇胎儿脐血高水平HBeAg可能和单核细胞PD-L1高表达有关,可能和新生儿HBV感染慢性化机制有关.
