目的:研究复合在壳聚糖/胶原支架上的重组人转化生长因子β1(recomb ined hum an transform ing growthfactorβ1,rhTGF-β1)的释放情况及人牙周膜成纤维细胞(hum an periodontal ligam ent fibro-b lasts,HPDLFs)在载体上三维培养的生...目的:研究复合在壳聚糖/胶原支架上的重组人转化生长因子β1(recomb ined hum an transform ing growthfactorβ1,rhTGF-β1)的释放情况及人牙周膜成纤维细胞(hum an periodontal ligam ent fibro-b lasts,HPDLFs)在载体上三维培养的生物学行为。方法:采用ELISA法检测rhTGF-β1的释放过程;原代培养HPDLFs,用MTT法检测及激光共聚焦显微镜观察HPDLFs在含有rhTGF-β1和不含rhTGF-β1的壳聚糖/胶原支架上的增殖情况。结果:ELISA显示复合在壳聚糖/胶原支架上rhTGF-β1的释放,可持续至4周。MTT结果与电镜扫描分析均显示HP-DLFs在两种不同支架上的增殖有显著性差异(P<0.5)。结论:复合在支架上的TGF-β1对HPDLFs的增殖有较好的促进作用。该复合支架能在一定程度上起到控释TGF-β1作用,但控释持续时间仍显不足。展开更多
In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone int...In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone into the ischemic area in animal models, and compared their effects. At 14 days after co-transplantation of bone marrow mesenchymal stem cells and the hi- tosan-collagen scaffold, neurological function recovered noticeably. Vascular endothelial growth factor expression and nestin-labeled neural precursor cells were detected in the iscbemic area, surrounding tissue, hippocampal dentate gyrus and subventricular zone. Simultaneously, a high level of expression of glial fibrillary acidic protein and a low level of expression of neuron-spe- cific enolase were visible in BrdU-labeled bone marrow mesenchymal stem cells. These findings suggest that transplantation of a composite of bone marrow mesenchymal stem cells and a chi- tosan-collagen scaffold has a neuroprotective effect following ischemic stroke.展开更多
文摘目的:研究复合在壳聚糖/胶原支架上的重组人转化生长因子β1(recomb ined hum an transform ing growthfactorβ1,rhTGF-β1)的释放情况及人牙周膜成纤维细胞(hum an periodontal ligam ent fibro-b lasts,HPDLFs)在载体上三维培养的生物学行为。方法:采用ELISA法检测rhTGF-β1的释放过程;原代培养HPDLFs,用MTT法检测及激光共聚焦显微镜观察HPDLFs在含有rhTGF-β1和不含rhTGF-β1的壳聚糖/胶原支架上的增殖情况。结果:ELISA显示复合在壳聚糖/胶原支架上rhTGF-β1的释放,可持续至4周。MTT结果与电镜扫描分析均显示HP-DLFs在两种不同支架上的增殖有显著性差异(P<0.5)。结论:复合在支架上的TGF-β1对HPDLFs的增殖有较好的促进作用。该复合支架能在一定程度上起到控释TGF-β1作用,但控释持续时间仍显不足。
基金funded by a grant from Shaanxi Provincial Support Project of Scientific Research Development Plan of China,No.2012KCT-16
文摘In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone into the ischemic area in animal models, and compared their effects. At 14 days after co-transplantation of bone marrow mesenchymal stem cells and the hi- tosan-collagen scaffold, neurological function recovered noticeably. Vascular endothelial growth factor expression and nestin-labeled neural precursor cells were detected in the iscbemic area, surrounding tissue, hippocampal dentate gyrus and subventricular zone. Simultaneously, a high level of expression of glial fibrillary acidic protein and a low level of expression of neuron-spe- cific enolase were visible in BrdU-labeled bone marrow mesenchymal stem cells. These findings suggest that transplantation of a composite of bone marrow mesenchymal stem cells and a chi- tosan-collagen scaffold has a neuroprotective effect following ischemic stroke.
