Restraint water-immersion stress(RWIS), a compound stress model, has been widely used to induce acute gastric ulceration in rats. A wealth of evidence suggests that the central nucleus of the amygdala(CEA) is a focal ...Restraint water-immersion stress(RWIS), a compound stress model, has been widely used to induce acute gastric ulceration in rats. A wealth of evidence suggests that the central nucleus of the amygdala(CEA) is a focal region for mediating the biological response to stress. Different stressors induce distinct alterations of neuronal activity in the CEA; however, few studies have reported the characteristics of CEA neuronal activity induced by RWIS. Therefore, we explored this issue using immunohistochemistry and in vivo extracellular single-unit recording. Our results showed that RWIS and restraint stress(RS) differentially changed the c-Fos expression and firing properties of neurons in the medial CEA. In addition,RWIS, but not RS, induced the activation of corticotropinreleasing hormone neurons in the CEA. These findings suggested that specific neuronal activation in the CEA is involved in the formation of RWIS-induced gastric ulcers.This study also provides a possible theoretical explanation for the different gastric dysfunctions induced by different stressors.展开更多
本研究旨在探讨外源性给予杏仁核中央核区不同浓度的降钙素基因相关肽(calcitonin gene-related peptide,CGRP)对小鼠认知和学习记忆的影响。将30日龄C57BL/6J小鼠随机分为空白对照组、手术对照组、200 ng CGRP组、400 ng CGRP组和800 n...本研究旨在探讨外源性给予杏仁核中央核区不同浓度的降钙素基因相关肽(calcitonin gene-related peptide,CGRP)对小鼠认知和学习记忆的影响。将30日龄C57BL/6J小鼠随机分为空白对照组、手术对照组、200 ng CGRP组、400 ng CGRP组和800 ng CGRP组,每组10只。外源性给予双侧杏仁核中央核区不同浓度的CGRP后,用旷场实验检测各组小鼠认知功能,用新物体认知实验和Morris水迷宫实验检测各组小鼠学习记忆能力。旷场实验结果显示,与空白对照组相比,800 ng CGRP组小鼠的水平运动得分显著增加。新物体认知实验结果显示,与空白对照组相比,400 ng CGRP组的认知指数显著提高。Morris水迷宫实验结果显示,与空白对照组比较,400和800 ng CGRP组小鼠的潜伏期均显著缩短,穿越平台的次数显著增加;与200 ng CGRP组比较,400和800 ng CGRP组小鼠寻找平台的潜伏期均明显缩短。以上结果提示,双侧杏仁核注射CGRP可剂量依赖性促进小鼠非空间和空间学习记忆功能。展开更多
目的:阐明下丘脑室旁核(the paraventricu lar nucleus of hypothalam us,PVN)兴奋后的心血管反应及杏仁中央核(the central nucleus of am ygdala,CeA)在此心血管反应中的地位。方法:电刺激SD大鼠中枢核团PVN或CeA,或用核团内微量注射0...目的:阐明下丘脑室旁核(the paraventricu lar nucleus of hypothalam us,PVN)兴奋后的心血管反应及杏仁中央核(the central nucleus of am ygdala,CeA)在此心血管反应中的地位。方法:电刺激SD大鼠中枢核团PVN或CeA,或用核团内微量注射0.5 m o l/L L-谷氨酸(L-Sod iumG lutam ate,L-G lu)方法。同时记录大鼠股动脉血压、平均动脉压(m ean arterial pressure,MAP)、心电图及心率(heart rate,HR)。结果:电刺激一侧PVN后,MAP升高,HR变化不一,以下降为主。大鼠PVN电刺激或微量注射L-G lu均诱发升压反应,心率变化不一。待作用消失后,同侧CeA微量注射L-G lu100n l,注射后平均动脉压上升(10.27±1.80)mmHg,心率变化为(-10.66±8.11)次/m in。待作用消失后,在CeA微量注射0.02 m o l/L的红藻氨酸(KA)100 n l,10 m in后刺激PVN,血压升高(13.78±3.18)mmHg,较注射KA前削弱了(6.57±1.56)mmHg(P<0.05)。结论:PVN兴奋后引起以升压效应为主的心血管反应。杏仁中央核部分介导了其升压反应。展开更多
目的观察完全弗氏佐剂(complete Freund′s adjuvant,CFA)诱导的小鼠慢性炎症性疼痛模型中,单核细胞趋化因子(monocyte chemoattractant protein-1,MCP-1)及其受体CCR2在杏仁核脑区(the central nucleus of the amygdala,CeA)中的表达...目的观察完全弗氏佐剂(complete Freund′s adjuvant,CFA)诱导的小鼠慢性炎症性疼痛模型中,单核细胞趋化因子(monocyte chemoattractant protein-1,MCP-1)及其受体CCR2在杏仁核脑区(the central nucleus of the amygdala,CeA)中的表达变化以及细胞定位情况。方法足底皮下注射CFA 20μL,制作慢性炎症性疼痛模型,用行为学方法检测小鼠的机械性刺激缩爪阈值和热刺激缩爪潜伏期的变化;用real-time PCR法检测造模后不同时间点CeA中MCP-1/CCR2mRNA的表达变化;用Western blot法检测造模后不同时间点CeA中CCR2蛋白的表达变化;选择CFA 7天组,用免疫荧光染色来观察CeA中MCP-1/CCR2蛋白的表达情况,并用双标染色来定位表达MCP-1/CCR2的细胞类型。结果造模1h后小鼠同侧后爪机械性缩爪阈值和热缩爪潜伏期显著降低(P<0.001),并能维持7天以上;造模1天后,CeA中MCP-1mRNA表达量增加(P<0.05),7天达高峰(P<0.01),一直持续到14天(P<0.05);CCR2mRNA从造模6h起表达增加(P<0.05);Western blot结果显示CeA中CCR2蛋白在造模后各个时间点均有表达上调,7天达高峰(P<0.01),一直持续到14天(P<0.01);免疫荧光染色显示,CFA 7天组CeA中MCP-1/CCR2蛋白的表达与生理盐水对照组相比均有增加,而且荧光双标染色显示MCP-1/CCR2蛋白主要与神经元核标记物(NeuN)共标。结论足底皮下注射CFA能诱导小鼠产生机械性触诱发痛和热痛觉过敏,CeA中MCP-1/CCR2mRNA和蛋白表达均增加,且均在神经元表达,提示该部位表达增加的MCP-1/CCR2可能参与炎症性疼痛的维持过程。展开更多
基金supported by the National Natural Science Foundation of China (31571104 and 81501149)the Science and Technological Project of Shandong Province of China (2016GSF201058)
文摘Restraint water-immersion stress(RWIS), a compound stress model, has been widely used to induce acute gastric ulceration in rats. A wealth of evidence suggests that the central nucleus of the amygdala(CEA) is a focal region for mediating the biological response to stress. Different stressors induce distinct alterations of neuronal activity in the CEA; however, few studies have reported the characteristics of CEA neuronal activity induced by RWIS. Therefore, we explored this issue using immunohistochemistry and in vivo extracellular single-unit recording. Our results showed that RWIS and restraint stress(RS) differentially changed the c-Fos expression and firing properties of neurons in the medial CEA. In addition,RWIS, but not RS, induced the activation of corticotropinreleasing hormone neurons in the CEA. These findings suggested that specific neuronal activation in the CEA is involved in the formation of RWIS-induced gastric ulcers.This study also provides a possible theoretical explanation for the different gastric dysfunctions induced by different stressors.
基金supported by the National Natural Science Foundation of China(No.81373852)the Key Scientific Research Project of Colleges and Universities in Henan Province+2 种基金China(No.15A310019)PhD Research Fund of Henan University of Traditional Chinese MedicineChina(No.BSJJ-201510)
文摘本研究旨在探讨外源性给予杏仁核中央核区不同浓度的降钙素基因相关肽(calcitonin gene-related peptide,CGRP)对小鼠认知和学习记忆的影响。将30日龄C57BL/6J小鼠随机分为空白对照组、手术对照组、200 ng CGRP组、400 ng CGRP组和800 ng CGRP组,每组10只。外源性给予双侧杏仁核中央核区不同浓度的CGRP后,用旷场实验检测各组小鼠认知功能,用新物体认知实验和Morris水迷宫实验检测各组小鼠学习记忆能力。旷场实验结果显示,与空白对照组相比,800 ng CGRP组小鼠的水平运动得分显著增加。新物体认知实验结果显示,与空白对照组相比,400 ng CGRP组的认知指数显著提高。Morris水迷宫实验结果显示,与空白对照组比较,400和800 ng CGRP组小鼠的潜伏期均显著缩短,穿越平台的次数显著增加;与200 ng CGRP组比较,400和800 ng CGRP组小鼠寻找平台的潜伏期均明显缩短。以上结果提示,双侧杏仁核注射CGRP可剂量依赖性促进小鼠非空间和空间学习记忆功能。
文摘目的:阐明下丘脑室旁核(the paraventricu lar nucleus of hypothalam us,PVN)兴奋后的心血管反应及杏仁中央核(the central nucleus of am ygdala,CeA)在此心血管反应中的地位。方法:电刺激SD大鼠中枢核团PVN或CeA,或用核团内微量注射0.5 m o l/L L-谷氨酸(L-Sod iumG lutam ate,L-G lu)方法。同时记录大鼠股动脉血压、平均动脉压(m ean arterial pressure,MAP)、心电图及心率(heart rate,HR)。结果:电刺激一侧PVN后,MAP升高,HR变化不一,以下降为主。大鼠PVN电刺激或微量注射L-G lu均诱发升压反应,心率变化不一。待作用消失后,同侧CeA微量注射L-G lu100n l,注射后平均动脉压上升(10.27±1.80)mmHg,心率变化为(-10.66±8.11)次/m in。待作用消失后,在CeA微量注射0.02 m o l/L的红藻氨酸(KA)100 n l,10 m in后刺激PVN,血压升高(13.78±3.18)mmHg,较注射KA前削弱了(6.57±1.56)mmHg(P<0.05)。结论:PVN兴奋后引起以升压效应为主的心血管反应。杏仁中央核部分介导了其升压反应。
文摘目的:观察神经病理性痛条件下细胞外信号调节激酶(extracellular singal-regulated kinase,ERK)对疼痛引起的负性情绪反应的影响。方法:应用Western blot和行为药理学方法,观察腰5脊神经结扎(L5 spinalnerve ligation,SNL)大鼠中央杏仁核外侧囊状部(latero-capsular division of central nucleus of amygdala,CeC)内ERK及磷酸化-ERK(phosphorylated-ERK,p-ERK)的表达情况及ERK磷酸化抑制剂对疼痛引起的负性情绪反应的影响。结果:SNL模型大鼠CeC内p-ERK的表达水平明显升高,与对照组相比,有统计学差异(P<0.05),而总ERK的表达水平则未见组间差异;用超声波检测仪可以检测到SNL大鼠超声发声明显增多,但腹膜腔注射ERK磷酸化的抑制剂U0126后其超声发声被显著抑制。结论:中央杏仁核外侧囊状部内ERK的激活参与了神经病理性痛引起的突触可塑性,在痛相关情绪的产生中发挥了重要作用。
