Six kinds of elicitors were prepared respectively from Neurospora crassa, Monascus purpureus, Sporobolomyces roseus, Rhodotorula rubra, Nocardia sp.N89 and Actinoplanes sp.A05. When Penicillium sp.PT95 was incubated i...Six kinds of elicitors were prepared respectively from Neurospora crassa, Monascus purpureus, Sporobolomyces roseus, Rhodotorula rubra, Nocardia sp.N89 and Actinoplanes sp.A05. When Penicillium sp.PT95 was incubated in Czapek’s agar plates containing appropriate amounts of elicitors, both its sclerotia biomass and carotenoid content accumulated in sclerotia were enhanced significantly (P<0.01). Among tested elicitors, the elicitors from the fungi N.crassa, M.purpureus, S.roseus and R.rubra were more effective than those from the actinomycetes Nocardia sp.N89 and Actinoplanes sp.A05; the elicitor from M.purpureus gave the highest carotenoid yield of 599μg /plate, 2.76 times higher than that of control. Every one of elicitors except that from M.purpureus could increase significantly the proportion of β-carotene in total carotenoids (P<0.01).展开更多
Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimula...Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimulation could significantly enhance the R. sphaeroides biomass production and carotenoid yield. The optimal biostimulant proportion was 40 μL（about 6.4 × 10^5CFU）. Through the use of biostimulation, chemical oxygen demand removal, R. sphaeroides biomass production, carotenoid concentration, and carotenoid yield were improved by 178%, 67%, 214%, and 70%, respectively. Theoretical analysis revealed that there were two possible reasons for such increases. One was that biostimulation enhanced the R. sphaeroides wastewater treatment efficiency. The other was that biostimulation significantly decreased the peroxidase activity in R. sphaeroides. The results showed that the highest peroxidase activity dropped by 87% and the induction ratio of the RSP_3419 gene was 3.1 with the addition of biostimulant. The enhanced carotenoid yield in R. sphaeroides could thus be explained by a decrease in peroxidase activity.展开更多
文摘Six kinds of elicitors were prepared respectively from Neurospora crassa, Monascus purpureus, Sporobolomyces roseus, Rhodotorula rubra, Nocardia sp.N89 and Actinoplanes sp.A05. When Penicillium sp.PT95 was incubated in Czapek’s agar plates containing appropriate amounts of elicitors, both its sclerotia biomass and carotenoid content accumulated in sclerotia were enhanced significantly (P<0.01). Among tested elicitors, the elicitors from the fungi N.crassa, M.purpureus, S.roseus and R.rubra were more effective than those from the actinomycetes Nocardia sp.N89 and Actinoplanes sp.A05; the elicitor from M.purpureus gave the highest carotenoid yield of 599μg /plate, 2.76 times higher than that of control. Every one of elicitors except that from M.purpureus could increase significantly the proportion of β-carotene in total carotenoids (P<0.01).
基金supported by the National Natural Science Foundation of China (No. 51278489)
文摘Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimulation could significantly enhance the R. sphaeroides biomass production and carotenoid yield. The optimal biostimulant proportion was 40 μL（about 6.4 × 10^5CFU）. Through the use of biostimulation, chemical oxygen demand removal, R. sphaeroides biomass production, carotenoid concentration, and carotenoid yield were improved by 178%, 67%, 214%, and 70%, respectively. Theoretical analysis revealed that there were two possible reasons for such increases. One was that biostimulation enhanced the R. sphaeroides wastewater treatment efficiency. The other was that biostimulation significantly decreased the peroxidase activity in R. sphaeroides. The results showed that the highest peroxidase activity dropped by 87% and the induction ratio of the RSP_3419 gene was 3.1 with the addition of biostimulant. The enhanced carotenoid yield in R. sphaeroides could thus be explained by a decrease in peroxidase activity.
