为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织...为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织的较优组合为2,4-D 0.6 m g/L,NAA 0.05或0.5 m g/L,愈伤组织诱导率分别达到35.56%和24.65%,在油茶花药愈伤组织诱导过程中,2,4-D有利于愈伤组织的形成,是最重要的因素;2,4-D、6-BA的混合使用更有利于愈伤组织的发生,并且两种激素适当的配比是提高愈伤组织诱导率的重要因素,0.5 m g/L的2,4-D与0.2 m g/L 6-BA组合是实验得出的较优水平,愈伤组织诱导率达到42.74%。展开更多
Different culture explants, including anther, young panicle, young embryo, and mature embryo, from 19 rice varieties were used for callus induction and green plantlet differentiation. The culture efficiency differed s...Different culture explants, including anther, young panicle, young embryo, and mature embryo, from 19 rice varieties were used for callus induction and green plantlet differentiation. The culture efficiency differed significantly among the four types of explants, and varied from genotype to genotype. Callus induction frequency presented significantly positive correlation each between anther and young panicle, anther and mature embryo, and young panicle and young embryo. Green plantlet differentiation showed no relationship between different types of explants. In addition, no relationship was found between callus induction frequency and green plantlet differentiation frequency.展开更多
DURING the last two decades, anther culture technique has been widely used in plant improve-ment including rice, wheat and maize. Recently, doubled haploid (DH) progenies derivedfrom anther culture have also been used...DURING the last two decades, anther culture technique has been widely used in plant improve-ment including rice, wheat and maize. Recently, doubled haploid (DH) progenies derivedfrom anther culture have also been used in molecular mapping projects as permanent popula-tions. Some studies have revealed that the rice culturability is controlled by a few genetic fac-tors. Xue suggested that it was an inheritable trait with additive gene effect. Wu展开更多
The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromat...The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromatography (HPLC). The results showed that the contents and ratio of endogenous hormones were one of the key factors affecting callus induction frequencies (CIF) and green plantlet differentiation frequencies (GPDF). The influence of endogenous hormones of different explants on CIF represented as: Zoatin ribosile (ZR) showed negative effect, indole-3-acetic acid (IAA) did positive effect, and gibberellic acid (GA) did negative effect except for mature embryos. The influence of endogenous hormones on green plantlet differentiation frequency (GPDF) showed: IAA and GA were negative effect; abscisic acid (ABA) and zeatin+ zeatin riboside (Z+ZR) were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z+ZR had a positive effect on CIF, and there was a notable positive correlation between Z+ZR/ IAA and GPDF, so was between ABA/IAA and GPDF.展开更多
文摘为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织的较优组合为2,4-D 0.6 m g/L,NAA 0.05或0.5 m g/L,愈伤组织诱导率分别达到35.56%和24.65%,在油茶花药愈伤组织诱导过程中,2,4-D有利于愈伤组织的形成,是最重要的因素;2,4-D、6-BA的混合使用更有利于愈伤组织的发生,并且两种激素适当的配比是提高愈伤组织诱导率的重要因素,0.5 m g/L的2,4-D与0.2 m g/L 6-BA组合是实验得出的较优水平,愈伤组织诱导率达到42.74%。
文摘Different culture explants, including anther, young panicle, young embryo, and mature embryo, from 19 rice varieties were used for callus induction and green plantlet differentiation. The culture efficiency differed significantly among the four types of explants, and varied from genotype to genotype. Callus induction frequency presented significantly positive correlation each between anther and young panicle, anther and mature embryo, and young panicle and young embryo. Green plantlet differentiation showed no relationship between different types of explants. In addition, no relationship was found between callus induction frequency and green plantlet differentiation frequency.
文摘DURING the last two decades, anther culture technique has been widely used in plant improve-ment including rice, wheat and maize. Recently, doubled haploid (DH) progenies derivedfrom anther culture have also been used in molecular mapping projects as permanent popula-tions. Some studies have revealed that the rice culturability is controlled by a few genetic fac-tors. Xue suggested that it was an inheritable trait with additive gene effect. Wu
文摘The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo) and calli with different culture capability were analyzed by means of high performance liquid chromatography (HPLC). The results showed that the contents and ratio of endogenous hormones were one of the key factors affecting callus induction frequencies (CIF) and green plantlet differentiation frequencies (GPDF). The influence of endogenous hormones of different explants on CIF represented as: Zoatin ribosile (ZR) showed negative effect, indole-3-acetic acid (IAA) did positive effect, and gibberellic acid (GA) did negative effect except for mature embryos. The influence of endogenous hormones on green plantlet differentiation frequency (GPDF) showed: IAA and GA were negative effect; abscisic acid (ABA) and zeatin+ zeatin riboside (Z+ZR) were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z+ZR had a positive effect on CIF, and there was a notable positive correlation between Z+ZR/ IAA and GPDF, so was between ABA/IAA and GPDF.
