目的 探讨右美托咪定联合七氟醚麻醉对老年下肢骨折患者术后认知功能、应激反应及疼痛的影响。方法 将2015年12月—2017年12月118例于我院行手术治疗的老年下肢骨折患者作为研究对象,根据麻醉药物使用的不同分为观察组( n =62)和对照组(...目的 探讨右美托咪定联合七氟醚麻醉对老年下肢骨折患者术后认知功能、应激反应及疼痛的影响。方法 将2015年12月—2017年12月118例于我院行手术治疗的老年下肢骨折患者作为研究对象,根据麻醉药物使用的不同分为观察组( n =62)和对照组( n =56),观察组予右美托咪定联合七氟醚麻醉,对照组予右美托咪定联合丙泊酚麻醉。比较两组术前(T1)、术后6 h(T2)、术后12 h(T3)和术后24 h(T4)时认知功能、应激反应及疼痛情况。结果 对照组T2、T3、T4时简易精神状态量表(mini-Mental state examination, MMSE)评分均低于T1时,观察组T2、T3时MMSE评分较T1时降低,比较差异均有统计学意义( P <0.01);观察组T2、T3和T4时MMSE评分均显著高于对照组( P <0.01)。两组T2、T3和T4时丙二醛(MDA)、活性氧簇(ROS)含量均较T1时升高,且观察组T2、T3和T4时MDA、ROS含量均低于对照组,差异均有统计学意义( P <0.01)。两组T2、T3和T4时视觉模拟评分(visual analogue scale, VAS)均较T1时显著降低,且观察组T2、T3和T4时VAS评分均显著低于对照组,差异均有统计学意义( P <0.01)。结论 右美托咪定联合七氟醚麻醉更有利于保护老年下肢骨折患者术后认知功能,并抑制机体应激反应的产生,可更大程度减轻术后疼痛,可作为老年下肢骨折手术适用的麻醉方案。展开更多
Objective： To investigate the effects and possible mechanism of Panax Notoginseng saponins （PNS） on oxidative stress-induced damage and apoptosis in bone marrow stromal cells （BMSCs）.Methods： BMSCs were isolated...Objective： To investigate the effects and possible mechanism of Panax Notoginseng saponins （PNS） on oxidative stress-induced damage and apoptosis in bone marrow stromal cells （BMSCs）.Methods： BMSCs were isolated and cultured from 2-month-old New Zealand rabbits by the density gradient centrifugation combined with adherent method.The third passage cells were used for subsequent experiments.Oxidative stress was induced in cultured BMSCs by H2O2 （0.1 mmol/L）.BMSCs were pretreated with 25-200 μg/mL PNS for 4 h before H2O2 treatment.Proliferation of BMSCs was observed using MTT assay.Alkaline phosphatase （ALP） activity,as an index of early osteoblastic differentiation,was determined with an ALP assay kit.Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/ propidium iodide.Oxidative stress level was examined by reactive oxygen species （ROS） assay.The protein expressions of Bax,Bcl-2 and Caspase-3 in BMSCs were analyzed by Western blotting.Results： PNS had different concentrationdependent effects on proliferation and osteoblast differentiation of BMSCs induced by H2O2.A PNS concentration of 100 μg/mL was determined as the optimal effective concentration.PNS markedly attenuated H2O2-induced apoptosis rate from 41.91% to 14.67% （P〈0.01）.PNS significantly decreased ROS level induced by H2O2 （P〈0.01）.Furthermore,pretreatment with PNS significantly reversed H2O2-induced inhibition of Bcl-2 expression and augmentation of Bax and Caspase-3 expression （P〈0.01）.Conclusion： PNS had a protective effect on oxidative stress-induced damage and apoptosis in cultured rabbit BMSCs through scavenging ROS and regulating the Bcl-2/Bax pathway.展开更多
文摘目的 探讨右美托咪定联合七氟醚麻醉对老年下肢骨折患者术后认知功能、应激反应及疼痛的影响。方法 将2015年12月—2017年12月118例于我院行手术治疗的老年下肢骨折患者作为研究对象,根据麻醉药物使用的不同分为观察组( n =62)和对照组( n =56),观察组予右美托咪定联合七氟醚麻醉,对照组予右美托咪定联合丙泊酚麻醉。比较两组术前(T1)、术后6 h(T2)、术后12 h(T3)和术后24 h(T4)时认知功能、应激反应及疼痛情况。结果 对照组T2、T3、T4时简易精神状态量表(mini-Mental state examination, MMSE)评分均低于T1时,观察组T2、T3时MMSE评分较T1时降低,比较差异均有统计学意义( P <0.01);观察组T2、T3和T4时MMSE评分均显著高于对照组( P <0.01)。两组T2、T3和T4时丙二醛(MDA)、活性氧簇(ROS)含量均较T1时升高,且观察组T2、T3和T4时MDA、ROS含量均低于对照组,差异均有统计学意义( P <0.01)。两组T2、T3和T4时视觉模拟评分(visual analogue scale, VAS)均较T1时显著降低,且观察组T2、T3和T4时VAS评分均显著低于对照组,差异均有统计学意义( P <0.01)。结论 右美托咪定联合七氟醚麻醉更有利于保护老年下肢骨折患者术后认知功能,并抑制机体应激反应的产生,可更大程度减轻术后疼痛,可作为老年下肢骨折手术适用的麻醉方案。
基金Supported by National Natural Science Foundation of China(No.30600624)
文摘Objective： To investigate the effects and possible mechanism of Panax Notoginseng saponins （PNS） on oxidative stress-induced damage and apoptosis in bone marrow stromal cells （BMSCs）.Methods： BMSCs were isolated and cultured from 2-month-old New Zealand rabbits by the density gradient centrifugation combined with adherent method.The third passage cells were used for subsequent experiments.Oxidative stress was induced in cultured BMSCs by H2O2 （0.1 mmol/L）.BMSCs were pretreated with 25-200 μg/mL PNS for 4 h before H2O2 treatment.Proliferation of BMSCs was observed using MTT assay.Alkaline phosphatase （ALP） activity,as an index of early osteoblastic differentiation,was determined with an ALP assay kit.Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/ propidium iodide.Oxidative stress level was examined by reactive oxygen species （ROS） assay.The protein expressions of Bax,Bcl-2 and Caspase-3 in BMSCs were analyzed by Western blotting.Results： PNS had different concentrationdependent effects on proliferation and osteoblast differentiation of BMSCs induced by H2O2.A PNS concentration of 100 μg/mL was determined as the optimal effective concentration.PNS markedly attenuated H2O2-induced apoptosis rate from 41.91% to 14.67% （P〈0.01）.PNS significantly decreased ROS level induced by H2O2 （P〈0.01）.Furthermore,pretreatment with PNS significantly reversed H2O2-induced inhibition of Bcl-2 expression and augmentation of Bax and Caspase-3 expression （P〈0.01）.Conclusion： PNS had a protective effect on oxidative stress-induced damage and apoptosis in cultured rabbit BMSCs through scavenging ROS and regulating the Bcl-2/Bax pathway.
