目的探讨急性脑出血患者血清非对称二甲基精氨酸(ADMA)水+与其病情及预后的关系。方法选择2015年4月-2018年2月在中国人民解放军联勤保障部队第九六六医院就诊的97例急性脑出血患者为研究对象。择同期体检但未发生急性脑出血的82例受试...目的探讨急性脑出血患者血清非对称二甲基精氨酸(ADMA)水+与其病情及预后的关系。方法选择2015年4月-2018年2月在中国人民解放军联勤保障部队第九六六医院就诊的97例急性脑出血患者为研究对象。择同期体检但未发生急性脑出血的82例受试者作为阴性对照组。比较两组受试者血清ADMA水平。分析ADMA与急性脑出血患者病情及预后的关系。结果急性脑出血组患者血清ADMA水平高于阴性对照组[0.57(0.43,0.74)μmol/L vs 0.42(0.33,0.51)μmol/L],差异有统计学意义(P<0.05)。急性脑出血患者血清AD-MA水平与NIHSS评分、血肿量及niRS评分均呈正相关关系。预后不良组和预后良好组患者在性别、吸烟史、高血压史、糖尿病史和冠心病史方面比较,差异不具统计学意义(P>0.05);在年龄、NIHSS评分、血肿量、早期神经功能恶化及ADMA水平方面比较,差异有统计学意义(P<0.05).Logisric回归分析显示年龄>65岁、NIHSS评分>12分、血肿量>28 mL、早期神经功能恶化及ADMA>0.58与急性脑出血患者预后不良因素密切相关。ADMA诊断急性脑出血患者预后不良的ROC AUC为0.867(95%CT:0.782~0.927),效能较高。结论急性脑出血患者血清ADMA水平升高,且与NIHSS评分、血肿量及mRS评分呈正相关关系,可作为急性脑出血患者病情及预后判断的标志物。展开更多
Background: Preeclampsia is a worldwide pregnancy complication, and early identification of patients with an increased risk is one of the key goals in obstetrics. First trimester screening is crucial over the second t...Background: Preeclampsia is a worldwide pregnancy complication, and early identification of patients with an increased risk is one of the key goals in obstetrics. First trimester screening is crucial over the second trimester for understanding the early onset of the disorder, with basal levels of the biochemical parameters associated with the underlying placentation process. Objective: The study aims to assess the levels of serum biochemical markers in pregnant women at first trimester, to evaluate statistical significance and correlation of the values in support of trophoblastic cell integrity, endothelial function and oxidative stress. Materials and Methods: A longitudinal study was conducted on 86 pregnant women of age group 20 - 35 years, Primigravida with singleton pregnancy who visited prenatal check up between 11 - 13 weeks of gestation. Maternal sera was collected for screening Placental protein 13 (PP13), Caspase 3, Asymmetric dimethylarginine (ADMA), Nitric oxide (NO) by ELISA. Xanthine oxidase (XO) activity was assayed spectrophotometrically. Calcium and Uric acid (UA) were measured by dry chemistry analyser. Results: The mean ± SD values for mean arterial pressure (MAP) are 108.4 ± 18.9, UA 2.01 ± 0.85, Total oxidant status (TOS) 12.83 ± 5.17, Total antioxidant capacity (TAC) 24.10 ± 14.28, XO 1.01 ± 2.67, Caspase-3 1.76 ± 2.22, PP13 489.77 ± 53.6, Calcium 10.88 ± 1.97, ADMA 19.03 ± 17.08 and NO 1.16 ± 0.75. The statistical analysis by SPSS package version 20 revealed positive correlation between ADMA & Caspase-3 (r = +0.435), PP13 & NO (r = +0.241), TOS & TAC (r = +0.176), UA & ADMA (r = +0.176), UA & TAC (r = +0.168) and negative correlation between PP13 & ADMA (r = -0.158), NO & TOS (r = -0.114), UA & XO (r = -0.173), UA & NO (r = -0.186), UA & Caspase 3 (r = -0.106) and MAP & Calcium (r = -0.303). Conclusion: The study concludes that first trimester biochemical markers and their correlation predict the trophoblastic cell integrity and endothelial function during placentation under preva展开更多
Cardiac Allograft Vasculopathy, an accelerated form of arterial occlusive disease, is the major cause of death in the long-term after heart transplantation. Multiple factors influence the initiation and progression of...Cardiac Allograft Vasculopathy, an accelerated form of arterial occlusive disease, is the major cause of death in the long-term after heart transplantation. Multiple factors influence the initiation and progression of CAV. These include ischemia-reperfusion, dyslipidemia, insulin resistance, and hypertension due to the use of immunosuppressive agents, the direct effects of immunosuppressive agents on endothelial function, and viruses (CMV). Impaired endothelial function reflects abnormalities in the production or activity of several vasoactive substances. Disruption of the nitric oxide synthase (NOS) pathway leads to changes in vascular reactivity, structure, and interaction with circulating blood components. Since endothelium-derived nitric oxide (NO) suppresses vascular cell proliferation and vascular inflammation, a deficit in vascular NO facilitates development of CAV. The link between oxidative stress and CAV largely exists in the balance between free radical superoxide generation and NO production. This review focuses on identifying the oxidative stress factors affecting CAV.展开更多
文摘目的探讨急性脑出血患者血清非对称二甲基精氨酸(ADMA)水+与其病情及预后的关系。方法选择2015年4月-2018年2月在中国人民解放军联勤保障部队第九六六医院就诊的97例急性脑出血患者为研究对象。择同期体检但未发生急性脑出血的82例受试者作为阴性对照组。比较两组受试者血清ADMA水平。分析ADMA与急性脑出血患者病情及预后的关系。结果急性脑出血组患者血清ADMA水平高于阴性对照组[0.57(0.43,0.74)μmol/L vs 0.42(0.33,0.51)μmol/L],差异有统计学意义(P<0.05)。急性脑出血患者血清AD-MA水平与NIHSS评分、血肿量及niRS评分均呈正相关关系。预后不良组和预后良好组患者在性别、吸烟史、高血压史、糖尿病史和冠心病史方面比较,差异不具统计学意义(P>0.05);在年龄、NIHSS评分、血肿量、早期神经功能恶化及ADMA水平方面比较,差异有统计学意义(P<0.05).Logisric回归分析显示年龄>65岁、NIHSS评分>12分、血肿量>28 mL、早期神经功能恶化及ADMA>0.58与急性脑出血患者预后不良因素密切相关。ADMA诊断急性脑出血患者预后不良的ROC AUC为0.867(95%CT:0.782~0.927),效能较高。结论急性脑出血患者血清ADMA水平升高,且与NIHSS评分、血肿量及mRS评分呈正相关关系,可作为急性脑出血患者病情及预后判断的标志物。
文摘Background: Preeclampsia is a worldwide pregnancy complication, and early identification of patients with an increased risk is one of the key goals in obstetrics. First trimester screening is crucial over the second trimester for understanding the early onset of the disorder, with basal levels of the biochemical parameters associated with the underlying placentation process. Objective: The study aims to assess the levels of serum biochemical markers in pregnant women at first trimester, to evaluate statistical significance and correlation of the values in support of trophoblastic cell integrity, endothelial function and oxidative stress. Materials and Methods: A longitudinal study was conducted on 86 pregnant women of age group 20 - 35 years, Primigravida with singleton pregnancy who visited prenatal check up between 11 - 13 weeks of gestation. Maternal sera was collected for screening Placental protein 13 (PP13), Caspase 3, Asymmetric dimethylarginine (ADMA), Nitric oxide (NO) by ELISA. Xanthine oxidase (XO) activity was assayed spectrophotometrically. Calcium and Uric acid (UA) were measured by dry chemistry analyser. Results: The mean ± SD values for mean arterial pressure (MAP) are 108.4 ± 18.9, UA 2.01 ± 0.85, Total oxidant status (TOS) 12.83 ± 5.17, Total antioxidant capacity (TAC) 24.10 ± 14.28, XO 1.01 ± 2.67, Caspase-3 1.76 ± 2.22, PP13 489.77 ± 53.6, Calcium 10.88 ± 1.97, ADMA 19.03 ± 17.08 and NO 1.16 ± 0.75. The statistical analysis by SPSS package version 20 revealed positive correlation between ADMA & Caspase-3 (r = +0.435), PP13 & NO (r = +0.241), TOS & TAC (r = +0.176), UA & ADMA (r = +0.176), UA & TAC (r = +0.168) and negative correlation between PP13 & ADMA (r = -0.158), NO & TOS (r = -0.114), UA & XO (r = -0.173), UA & NO (r = -0.186), UA & Caspase 3 (r = -0.106) and MAP & Calcium (r = -0.303). Conclusion: The study concludes that first trimester biochemical markers and their correlation predict the trophoblastic cell integrity and endothelial function during placentation under preva
文摘Cardiac Allograft Vasculopathy, an accelerated form of arterial occlusive disease, is the major cause of death in the long-term after heart transplantation. Multiple factors influence the initiation and progression of CAV. These include ischemia-reperfusion, dyslipidemia, insulin resistance, and hypertension due to the use of immunosuppressive agents, the direct effects of immunosuppressive agents on endothelial function, and viruses (CMV). Impaired endothelial function reflects abnormalities in the production or activity of several vasoactive substances. Disruption of the nitric oxide synthase (NOS) pathway leads to changes in vascular reactivity, structure, and interaction with circulating blood components. Since endothelium-derived nitric oxide (NO) suppresses vascular cell proliferation and vascular inflammation, a deficit in vascular NO facilitates development of CAV. The link between oxidative stress and CAV largely exists in the balance between free radical superoxide generation and NO production. This review focuses on identifying the oxidative stress factors affecting CAV.
