Objective：To study the effects of Erlong Zuoci Pill（耳聋左慈丸,ELZCP）and its disassembled prescriptions on gentamicin（GM）-induced ototoxicity model in vitro.Methods：After the spiral organ of cochleae of newborn ...Objective：To study the effects of Erlong Zuoci Pill（耳聋左慈丸,ELZCP）and its disassembled prescriptions on gentamicin（GM）-induced ototoxicity model in vitro.Methods：After the spiral organ of cochleae of newborn mice（postnatal days：2-3）cultured for 24 h,GM alone or combined with water extracting-alcohol precipitating solution of ELZCP or with its disassembled prescriptions was added.Hair cells were observed under a fluorescence microscope after TRITC-phalloidin staining,and the cochlear hair cell loss rate was calculated by counting the whole cochlear hair cells and analyzed by whole cochlear hair cells analyzing software.Results： GM induced cochlear outer hair cells（OHCs）and inner hair cells（IHCs）injuries in a dose-dependent manner, and they were significantly different as compared with those in the normal control group（P〈0.05,P〈0.01）. ELZCP at the concentration of 0.003-3 mg/mL could decrease the hair cells loss induced by the 0.3 mmol/L GM （P〈0.05,P〈0.01）,the effects was in a dose-dependent manner,and the concentration of 0.3 mg/mL showed the optimal protective effect.For the ELZCP disassembled prescriptions,Liuwei-Dihuang could decrease OHC loss rate than that in the 0.3 mmol/L GM model group（P〈0.05）,but the OHC loss rate was still higher than that in the ELZCP group（P〈0.01）,which indicated that the protective effect of hair cells by Liuwei-Dihuang was not better than that of ELZCP.Poria decreased OHC loss rate from 72.1%±3.7%to 58.8%±8.2%（P〈0.05）. Conclusions：ELZCP could play a role in antagonizing the injury of cochlear hair cells induced by GM ototoxicity, and its disassembled prescriptions,Liuwei-Dihuang was the main component to protect the cochlear hair cells from GM-induced ototoxicity,and Magnetitum combined with Radix Bupleurui could strengthen the action of the whole prescription; Poria could reduce GM-induced OHC loss.展开更多
目的:探讨耳聋左慈丸联合通气散加减味治疗慢性分泌性中耳炎(CSOM)肝肾阴虚证的疗效以及对血清水通道蛋白(AQP)^(-1)和AQP-4水平的影响。方法:慢性中耳炎患者共117例,参照数字表法随机分为治疗组58例和对照组59例;对照组给予盐酸头孢美...目的:探讨耳聋左慈丸联合通气散加减味治疗慢性分泌性中耳炎(CSOM)肝肾阴虚证的疗效以及对血清水通道蛋白(AQP)^(-1)和AQP-4水平的影响。方法:慢性中耳炎患者共117例,参照数字表法随机分为治疗组58例和对照组59例;对照组给予盐酸头孢美特酯分散片治疗,500 mg/次,2次/d;枸地氯雷他定片,1片/次,1次/d;噻洛唑啉鼻腔滴鼻剂,2滴/次,2次/d。治疗组在对照组治疗的基础上给予耳聋左慈丸联合通气散加减治疗,1剂/d,常规水煎煮,分早晚2次内服;两组患者均给予4周治疗。比较两组中医症状积分和各频率下的骨导阈值;检测两组患者血清AQP^(-1)和AQP-4水平。结果:治疗组治疗后中医临床症状积分均明显低于对照组(P<0.01);治疗组患者临床总有效率为89.61%,对照组为76.92%,治疗组总有效率高于对照组(P<0.05);治疗组治疗后各频率(1,2,4,8 k Hz)骨导阈值均明显低于对照组(P<0.01);治疗后治疗组血清AQP^(-1)和AQP-4水平均明显高于对照组,比较差异均有统计学意义(P<0.01)。结论:耳聋左慈丸联合通气散加减治疗CSOM肝肾阴虚证可明显改善患者临床症状,降低骨导阈值,上调血清AQP^(-1)和AQP-4水平。展开更多
目的:用血清药理学方法观察耳聋左慈汤对庆大霉素(Gentamicin,GM)所致小鼠耳蜗毛细胞损伤的作用。方法:完整分离小鼠耳蜗基底膜,平铺于鼠尾胶上,无血清培养液培养24小时后,正常组更换含10%空白血清的培养液,模型组同时加入0.3mMGM和10%...目的:用血清药理学方法观察耳聋左慈汤对庆大霉素(Gentamicin,GM)所致小鼠耳蜗毛细胞损伤的作用。方法:完整分离小鼠耳蜗基底膜,平铺于鼠尾胶上,无血清培养液培养24小时后,正常组更换含10%空白血清的培养液,模型组同时加入0.3mMGM和10%空白血清,耳聋左慈汤药物血清组同时加入0.3 mM GM和10%含药血清,继续培养24小时,免疫荧光染色后荧光显微镜下进行全耳蜗毛细胞计数。用全耳蜗毛细胞损失率评价耳聋左慈汤含药血清对GM所致耳蜗毛细胞损伤的作用。结果:正常耳蜗毛细胞排列整齐,无缺失,而0.3mM GM模型组耳蜗毛细胞大量缺失,外毛细胞损失率(41.8±1.9)%高于内毛细胞损失率(16.8±1.8)%;耳聋左慈汤药物血清可降低耳蜗毛细胞的损失率,内、外毛细胞损失率与模型组比较差异均有统计学意义(P<0.05或P<0.01)。结论:耳聋左慈汤含药血清对GM造成的耳蜗毛细胞损伤有拮抗作用。展开更多
基金Supported by Shanghai Science and Technology Commission (No.04ZR14130)Leading Academic Discipline Project of Shanghai Municipal Education Commission(No.J50301)
文摘Objective：To study the effects of Erlong Zuoci Pill（耳聋左慈丸,ELZCP）and its disassembled prescriptions on gentamicin（GM）-induced ototoxicity model in vitro.Methods：After the spiral organ of cochleae of newborn mice（postnatal days：2-3）cultured for 24 h,GM alone or combined with water extracting-alcohol precipitating solution of ELZCP or with its disassembled prescriptions was added.Hair cells were observed under a fluorescence microscope after TRITC-phalloidin staining,and the cochlear hair cell loss rate was calculated by counting the whole cochlear hair cells and analyzed by whole cochlear hair cells analyzing software.Results： GM induced cochlear outer hair cells（OHCs）and inner hair cells（IHCs）injuries in a dose-dependent manner, and they were significantly different as compared with those in the normal control group（P〈0.05,P〈0.01）. ELZCP at the concentration of 0.003-3 mg/mL could decrease the hair cells loss induced by the 0.3 mmol/L GM （P〈0.05,P〈0.01）,the effects was in a dose-dependent manner,and the concentration of 0.3 mg/mL showed the optimal protective effect.For the ELZCP disassembled prescriptions,Liuwei-Dihuang could decrease OHC loss rate than that in the 0.3 mmol/L GM model group（P〈0.05）,but the OHC loss rate was still higher than that in the ELZCP group（P〈0.01）,which indicated that the protective effect of hair cells by Liuwei-Dihuang was not better than that of ELZCP.Poria decreased OHC loss rate from 72.1%±3.7%to 58.8%±8.2%（P〈0.05）. Conclusions：ELZCP could play a role in antagonizing the injury of cochlear hair cells induced by GM ototoxicity, and its disassembled prescriptions,Liuwei-Dihuang was the main component to protect the cochlear hair cells from GM-induced ototoxicity,and Magnetitum combined with Radix Bupleurui could strengthen the action of the whole prescription; Poria could reduce GM-induced OHC loss.
文摘目的:探讨耳聋左慈丸联合通气散加减味治疗慢性分泌性中耳炎(CSOM)肝肾阴虚证的疗效以及对血清水通道蛋白(AQP)^(-1)和AQP-4水平的影响。方法:慢性中耳炎患者共117例,参照数字表法随机分为治疗组58例和对照组59例;对照组给予盐酸头孢美特酯分散片治疗,500 mg/次,2次/d;枸地氯雷他定片,1片/次,1次/d;噻洛唑啉鼻腔滴鼻剂,2滴/次,2次/d。治疗组在对照组治疗的基础上给予耳聋左慈丸联合通气散加减治疗,1剂/d,常规水煎煮,分早晚2次内服;两组患者均给予4周治疗。比较两组中医症状积分和各频率下的骨导阈值;检测两组患者血清AQP^(-1)和AQP-4水平。结果:治疗组治疗后中医临床症状积分均明显低于对照组(P<0.01);治疗组患者临床总有效率为89.61%,对照组为76.92%,治疗组总有效率高于对照组(P<0.05);治疗组治疗后各频率(1,2,4,8 k Hz)骨导阈值均明显低于对照组(P<0.01);治疗后治疗组血清AQP^(-1)和AQP-4水平均明显高于对照组,比较差异均有统计学意义(P<0.01)。结论:耳聋左慈丸联合通气散加减治疗CSOM肝肾阴虚证可明显改善患者临床症状,降低骨导阈值,上调血清AQP^(-1)和AQP-4水平。
文摘目的:用血清药理学方法观察耳聋左慈汤对庆大霉素(Gentamicin,GM)所致小鼠耳蜗毛细胞损伤的作用。方法:完整分离小鼠耳蜗基底膜,平铺于鼠尾胶上,无血清培养液培养24小时后,正常组更换含10%空白血清的培养液,模型组同时加入0.3mMGM和10%空白血清,耳聋左慈汤药物血清组同时加入0.3 mM GM和10%含药血清,继续培养24小时,免疫荧光染色后荧光显微镜下进行全耳蜗毛细胞计数。用全耳蜗毛细胞损失率评价耳聋左慈汤含药血清对GM所致耳蜗毛细胞损伤的作用。结果:正常耳蜗毛细胞排列整齐,无缺失,而0.3mM GM模型组耳蜗毛细胞大量缺失,外毛细胞损失率(41.8±1.9)%高于内毛细胞损失率(16.8±1.8)%;耳聋左慈汤药物血清可降低耳蜗毛细胞的损失率,内、外毛细胞损失率与模型组比较差异均有统计学意义(P<0.05或P<0.01)。结论:耳聋左慈汤含药血清对GM造成的耳蜗毛细胞损伤有拮抗作用。
