目的研究银莱汤对发热大鼠体温和血清IL-1β、TNF-α、IL-6含量的影响。方法 90只SD大鼠连续测温3次,选取合格大鼠背部皮下注射20%干酵母混悬液10 m L/kg建立大鼠发热模型,随机平均分为正常组、模型组、阿司匹林组、银莱汤高剂量组、银...目的研究银莱汤对发热大鼠体温和血清IL-1β、TNF-α、IL-6含量的影响。方法 90只SD大鼠连续测温3次,选取合格大鼠背部皮下注射20%干酵母混悬液10 m L/kg建立大鼠发热模型,随机平均分为正常组、模型组、阿司匹林组、银莱汤高剂量组、银莱汤中剂量组、银莱汤低剂量组共6组,每组8只大鼠。分别于造模前0.5 h和造模后5 h两次灌胃给药,造模后每小时测温一次,于末次给药后3 h取血,通过ELISA和RIA方法检测血清中炎性介质白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子α(TNF-α)含量水平。结果银莱汤各剂量组体温均低于模型组(P<0.01),银莱汤各剂量组血清中IL-1β、TNF-α、IL-6含量均低于模型组(P<0.05)。结论银莱汤对发热大鼠具有明显的退热效果,其作用机制可能与降低机体炎症反应,减少血清中IL-1β、TNF-α、IL-6的产生或加速降解相关。展开更多
Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics analysis.Methods:Based on the Gene Expression Omnibus(GEO)database,lung tissue ...Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics analysis.Methods:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics analyses.In the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)groups.Mice in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 d.The mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 d.Pathological evaluation of the lung tissue was performed.Differentially expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics analyses.The drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)Tools.DEPs were verified by western blot.Results:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung tissue.The Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M groups.Enrichment analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative phosphorylation.The protein-protein interaction network revealed that Atp5a1,Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai decoction.Molecular docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to Atp5a1.Conclusion:The Yinlai decoction alleviated lung tissue damage and pneumonia in mice tha展开更多
Objective To investigate the effect of Yinlai Decoction(YD)on the microstructure of colon,and activity of D-lactic acid(DLA)and diamine oxidase(DAO)in serum of pneumonia mice model fed with high-calorie and high-prote...Objective To investigate the effect of Yinlai Decoction(YD)on the microstructure of colon,and activity of D-lactic acid(DLA)and diamine oxidase(DAO)in serum of pneumonia mice model fed with high-calorie and high-protein diet(HCD).Methods Sixty male Kunming mice were randomly divided into 6 groups by the random number table method:normal control,pneumonia,HCD,HCD with pneumonia(HCD-P),YD(229.2 mg/mL),and dexamethasone(15.63 mg/mL)groups,with 10 in each group.HCD mice were fed with 52%milk solution by gavage.Pneumonia mice was modeled with lipopolysaccharide inhalation and was fed by gavage with either the corresponding therapeutic drugs or saline water,twice daily,for 3 days.After hematoxylin-eosin staining,the changes in the colon structure were observed under light microscopy and transmission electron microscope,respectively.Enzyme-linked immunosorbent assay was used to detect the protein levels of DLA and DAO in the serum of mice.Results The colonic mucosal structure and ultrastructure of mice in the normal control group were clear and intact.The colonic mucosal goblet cells in the pneumonia group tended to increase,and the size of the microvilli varied.In the HCD-P group,the mucosal goblet cells showed a marked increase in size with increased secretory activity.Loose mucosal epithelial connections were also observed,as shown by widened intercellular gaps with short sparse microvilli.These pathological changes of intestinal mucosa were significantly reduced in mouse models with YD treatment,while there was no significant improvement after dexamethasone treatment.The serum DLA level was significantly higher in the pneumonia,HCD,and HCD-P groups as compared with the normal control group(P<0.05).Serum DLA was significantly lower in the YD group than HCD-P group(P<0.05).Moreover,serum DLA level significantly increased in the dexamethasone group as compared with the YD group(P<0.01).There was no statistical significance in the serum level of DAO among groups(P>0.05).Conclusions YD can protect function of intestinal 展开更多
Objective:Pediatric pneumonia is a common respiratory disease and Yinlai Decoction (YLD) is a commonly used treatment in China.We explored the anti-inflammatory mechanism of action of this traditional Chinese medicine...Objective:Pediatric pneumonia is a common respiratory disease and Yinlai Decoction (YLD) is a commonly used treatment in China.We explored the anti-inflammatory mechanism of action of this traditional Chinese medicine for pneumonia.Methods:We studied,by experimentation,the mechanism of action of YLD in treating pneumonia according to network pharmacology.By comparing YLD with dexamethasone (DXMS),we investigated the efficacy of YLD in treating pneumonia induced by lipopolysaccharide (LPS) in juvenile rats.Results:In an aqueous extract of YLD,22 chemical compounds were identified,among which 10 were related to inflammation,involving 78 target genes and 16 signaling pathways.Among them,45 core target proteins were related to biologic processes and functions,such as response to stimuli,biologic regulation,cell communication and protein binding.Animal experiments showed that YLD relieved pulmonary inflammation and demonstrated no significant damage to the liver,spleen or kidneys of rats.YLD could regulate expression of inflammatory cytokines in serum and inflammation-related proteins in lung tissues to some extent,but its effect is less significant than that of DXMS.Conclusions:YLD protected juvenile rats against LPS-induced pneumonia,and showed fewer side effects in comparison with DXMMS.YLD could be efficacious treatment for pediatric respiratory infections and even pneumonia.展开更多
基金supported by the National Natural Science Foundation of China(81874421)the Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(ZYYCXTD-C-202006).
文摘Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics analysis.Methods:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics analyses.In the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)groups.Mice in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 d.The mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 d.Pathological evaluation of the lung tissue was performed.Differentially expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics analyses.The drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)Tools.DEPs were verified by western blot.Results:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung tissue.The Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M groups.Enrichment analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative phosphorylation.The protein-protein interaction network revealed that Atp5a1,Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai decoction.Molecular docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to Atp5a1.Conclusion:The Yinlai decoction alleviated lung tissue damage and pneumonia in mice tha
基金Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No.ZYYCXTD-C-202006)。
文摘Objective To investigate the effect of Yinlai Decoction(YD)on the microstructure of colon,and activity of D-lactic acid(DLA)and diamine oxidase(DAO)in serum of pneumonia mice model fed with high-calorie and high-protein diet(HCD).Methods Sixty male Kunming mice were randomly divided into 6 groups by the random number table method:normal control,pneumonia,HCD,HCD with pneumonia(HCD-P),YD(229.2 mg/mL),and dexamethasone(15.63 mg/mL)groups,with 10 in each group.HCD mice were fed with 52%milk solution by gavage.Pneumonia mice was modeled with lipopolysaccharide inhalation and was fed by gavage with either the corresponding therapeutic drugs or saline water,twice daily,for 3 days.After hematoxylin-eosin staining,the changes in the colon structure were observed under light microscopy and transmission electron microscope,respectively.Enzyme-linked immunosorbent assay was used to detect the protein levels of DLA and DAO in the serum of mice.Results The colonic mucosal structure and ultrastructure of mice in the normal control group were clear and intact.The colonic mucosal goblet cells in the pneumonia group tended to increase,and the size of the microvilli varied.In the HCD-P group,the mucosal goblet cells showed a marked increase in size with increased secretory activity.Loose mucosal epithelial connections were also observed,as shown by widened intercellular gaps with short sparse microvilli.These pathological changes of intestinal mucosa were significantly reduced in mouse models with YD treatment,while there was no significant improvement after dexamethasone treatment.The serum DLA level was significantly higher in the pneumonia,HCD,and HCD-P groups as compared with the normal control group(P<0.05).Serum DLA was significantly lower in the YD group than HCD-P group(P<0.05).Moreover,serum DLA level significantly increased in the dexamethasone group as compared with the YD group(P<0.01).There was no statistical significance in the serum level of DAO among groups(P>0.05).Conclusions YD can protect function of intestinal
基金The authors are grateful for the financial support from the Fundamental Research Funds for the Central Universities(2017-JYB-XS-001)Beijing Nova Program(xx2018056,Z181100006218083)Beijing Municipal Natural Science Foundation(7172131).
文摘Objective:Pediatric pneumonia is a common respiratory disease and Yinlai Decoction (YLD) is a commonly used treatment in China.We explored the anti-inflammatory mechanism of action of this traditional Chinese medicine for pneumonia.Methods:We studied,by experimentation,the mechanism of action of YLD in treating pneumonia according to network pharmacology.By comparing YLD with dexamethasone (DXMS),we investigated the efficacy of YLD in treating pneumonia induced by lipopolysaccharide (LPS) in juvenile rats.Results:In an aqueous extract of YLD,22 chemical compounds were identified,among which 10 were related to inflammation,involving 78 target genes and 16 signaling pathways.Among them,45 core target proteins were related to biologic processes and functions,such as response to stimuli,biologic regulation,cell communication and protein binding.Animal experiments showed that YLD relieved pulmonary inflammation and demonstrated no significant damage to the liver,spleen or kidneys of rats.YLD could regulate expression of inflammatory cytokines in serum and inflammation-related proteins in lung tissues to some extent,but its effect is less significant than that of DXMS.Conclusions:YLD protected juvenile rats against LPS-induced pneumonia,and showed fewer side effects in comparison with DXMMS.YLD could be efficacious treatment for pediatric respiratory infections and even pneumonia.
