X-box-binding protein-1 （XBP-1） is an essential transcription factor in endoplasmic reticulum stress In this study, XBP-1 gene-transfected neural stem cells （NSCs） were transplanted into lesion sites to ensure sta...X-box-binding protein-1 （XBP-1） is an essential transcription factor in endoplasmic reticulum stress In this study, XBP-1 gene-transfected neural stem cells （NSCs） were transplanted into lesion sites to ensure stability and persistent expression of XBP-1, resulting in the exertion of anti-apoptotic effects. Simultaneously, XBP-1 gene transfection promotes the survival and differentiation of transplanted NSCs. Results from this study demonstrated that survival, proliferation and differentiation of XBP-1 g^ne-modified NSCs were enhanced when compared to unmodified NSCs at 28 days post-transplantation （P 〈 0.05）. A diminished number of apoptotic neural cells increased Bcl-2 expression and reduced Bax expression, and were observed in the ischemic region of the XBP-1-NSCs group （P 〈 0.05）. These results indicated that modification of the XBP-1 gene enhances the survival and migration of NSCs in vivo and decreases the occurrence of apoptosis.展开更多
X-box-binding protein 1-transfected neural stem cells were transplanted into the right lateral ventricles of rats with rotenone-induced Parkinson＇s disease. The survival capacities and differentiation rates of cells ...X-box-binding protein 1-transfected neural stem cells were transplanted into the right lateral ventricles of rats with rotenone-induced Parkinson＇s disease. The survival capacities and differentiation rates of cells expressing the dopaminergic marker tyrosine hydroxylase were higher in X-box-binding protein 1-transfected neural stem cells compared to non-transfected cells. Moreover, dopamine and 3,4-dihydroxyphenylacetic acid levels in the substantia nigra were significantly increased, α-synuclein expression was decreased, and neurological behaviors were significantly ameliorated in rats following transplantation of X-box-binding protein 1-transfected neural stem cells. These results indicate that transplantation of X-box-binding protein 1-transfected neural stem cells can promote stem cell survival and differentiation into dopaminergic neurons, increase dopamine and 3,4-dihydroxyphenylacetic acid levels, reduce α-synuclein aggregation in the substantia nigra, and improve the symptoms of Parkinson＇s disease in rats.展开更多
目的探讨慢性HBV感染者外周血白细胞内质网应激相关基因糖调节蛋白78（GRP78）、CCAAT增强子结合蛋白同源蛋白（CHOP）和X盒结合蛋白l（XBP1）m RNA水平及其与临床指标的相关性。方法采用实时荧光定量PCR技术检测40例正常人（NC）、43...目的探讨慢性HBV感染者外周血白细胞内质网应激相关基因糖调节蛋白78（GRP78）、CCAAT增强子结合蛋白同源蛋白（CHOP）和X盒结合蛋白l（XBP1）m RNA水平及其与临床指标的相关性。方法采用实时荧光定量PCR技术检测40例正常人（NC）、43例慢性无症状HBV携带者（ASC）、47例慢性乙型肝炎（CHB）、41例乙型肝炎肝硬化（LC）和35例乙型肝炎相关肝细胞癌（HCC）患者外周血白细胞GRP78、CHOP和XBP1 m RNA水平,分析其与病毒学和生化学指标的相关性。结果 ASC、CHB、LC和HCC患者外周血白细胞GRP78 m RNA水平和XBP1 m RNA水平分别为（0.58±0.53）和（0.76±0.57）、（0.59±0.67）和（0.50±0.64）、（1.07±1.09）和（1.44±1.00）和（1.17±1.69）和（0.90±1.33）,均显著高于健康人[分别为（0.01±0.61）和（0.01±0.97）,P〈0.01和P〈0.05];在HBs Ag〈1500IU/ml、1500-20000IU/ml和〉20000IU/ml组患者,GRP78 m RNA水平分别为（0.48±0.59）、（0.76±0.56）和（0.74±0.56）,三组间差异有统计学意义（P〈0.05）;HBV DNA水平为1×10^3-10^5copies/ml组XBP1 m RNA水平为（0.86±1.13）,显著低于HBV DNA〉1×105copies/ml组[（1.44±1.31）,P〈0.05];在不同转氨酶水平间、HBe Ag阳性与阴性组间和胆红素正常与升高组间,以上三种基因m RNA水平差异均无统计学意义（P〉0.05）。结论内质网应激可能与慢性HBV感染后的疾病进展相关。展开更多
文摘X-box-binding protein-1 （XBP-1） is an essential transcription factor in endoplasmic reticulum stress In this study, XBP-1 gene-transfected neural stem cells （NSCs） were transplanted into lesion sites to ensure stability and persistent expression of XBP-1, resulting in the exertion of anti-apoptotic effects. Simultaneously, XBP-1 gene transfection promotes the survival and differentiation of transplanted NSCs. Results from this study demonstrated that survival, proliferation and differentiation of XBP-1 g^ne-modified NSCs were enhanced when compared to unmodified NSCs at 28 days post-transplantation （P 〈 0.05）. A diminished number of apoptotic neural cells increased Bcl-2 expression and reduced Bax expression, and were observed in the ischemic region of the XBP-1-NSCs group （P 〈 0.05）. These results indicated that modification of the XBP-1 gene enhances the survival and migration of NSCs in vivo and decreases the occurrence of apoptosis.
文摘X-box-binding protein 1-transfected neural stem cells were transplanted into the right lateral ventricles of rats with rotenone-induced Parkinson＇s disease. The survival capacities and differentiation rates of cells expressing the dopaminergic marker tyrosine hydroxylase were higher in X-box-binding protein 1-transfected neural stem cells compared to non-transfected cells. Moreover, dopamine and 3,4-dihydroxyphenylacetic acid levels in the substantia nigra were significantly increased, α-synuclein expression was decreased, and neurological behaviors were significantly ameliorated in rats following transplantation of X-box-binding protein 1-transfected neural stem cells. These results indicate that transplantation of X-box-binding protein 1-transfected neural stem cells can promote stem cell survival and differentiation into dopaminergic neurons, increase dopamine and 3,4-dihydroxyphenylacetic acid levels, reduce α-synuclein aggregation in the substantia nigra, and improve the symptoms of Parkinson＇s disease in rats.
文摘目的探讨慢性HBV感染者外周血白细胞内质网应激相关基因糖调节蛋白78（GRP78）、CCAAT增强子结合蛋白同源蛋白（CHOP）和X盒结合蛋白l（XBP1）m RNA水平及其与临床指标的相关性。方法采用实时荧光定量PCR技术检测40例正常人（NC）、43例慢性无症状HBV携带者（ASC）、47例慢性乙型肝炎（CHB）、41例乙型肝炎肝硬化（LC）和35例乙型肝炎相关肝细胞癌（HCC）患者外周血白细胞GRP78、CHOP和XBP1 m RNA水平,分析其与病毒学和生化学指标的相关性。结果 ASC、CHB、LC和HCC患者外周血白细胞GRP78 m RNA水平和XBP1 m RNA水平分别为（0.58±0.53）和（0.76±0.57）、（0.59±0.67）和（0.50±0.64）、（1.07±1.09）和（1.44±1.00）和（1.17±1.69）和（0.90±1.33）,均显著高于健康人[分别为（0.01±0.61）和（0.01±0.97）,P〈0.01和P〈0.05];在HBs Ag〈1500IU/ml、1500-20000IU/ml和〉20000IU/ml组患者,GRP78 m RNA水平分别为（0.48±0.59）、（0.76±0.56）和（0.74±0.56）,三组间差异有统计学意义（P〈0.05）;HBV DNA水平为1×10^3-10^5copies/ml组XBP1 m RNA水平为（0.86±1.13）,显著低于HBV DNA〉1×105copies/ml组[（1.44±1.31）,P〈0.05];在不同转氨酶水平间、HBe Ag阳性与阴性组间和胆红素正常与升高组间,以上三种基因m RNA水平差异均无统计学意义（P〉0.05）。结论内质网应激可能与慢性HBV感染后的疾病进展相关。
