Recombinant virus-vectored vaccines are novel agents that can effectively activate specific and nonspecific immunity,are multivalent and multieffective,and have high safety ratings.Animal alphaherpesviruses have a lar...Recombinant virus-vectored vaccines are novel agents that can effectively activate specific and nonspecific immunity,are multivalent and multieffective,and have high safety ratings.Animal alphaherpesviruses have a large genome,contain multiple nonessential regions that do not affect viral replication and are capable of accepting the insertion of an exogenous gene and expressing the antigen protein.Furthermore,animal alphaherpesviruses have a wide host spectrum,can replicate in the host and continuously stimulate the animal to produce immunity to the corresponding pathogen,thus making them ideal carriers for recombinant virus-vectored vaccines.With the development of gene-editing technology,recombinant viruses capable of expressing foreign genes can be constructed by various methods.Currently,studies on recombinant virusvectored vaccines constructed based on animal alphaherpesviruses have involved poultry,pigs,cattle,sheep,and companion animals.Studies have shown that the construction of recombinant animal alphaherpesviruses enables the acquisition of immunity to multiple diseases.This article mainly summarizes the current progress on animal alphaherpesvirus-vectored vaccines,aiming to provide reference for the development of new animal alphaherpesvirus-vectored vaccines.展开更多
目的构建一种重组新型冠状病毒疫苗(腺病毒载体)病毒颗粒数微滴式数字PCR(droplet digital PCR,ddPCR)检测方法。方法以新型冠状病毒BA.1株基因组为靶基因,选取刺突蛋白保守序列设计引物和探针,建立重组新型冠状病毒疫苗病毒颗粒数ddPC...目的构建一种重组新型冠状病毒疫苗(腺病毒载体)病毒颗粒数微滴式数字PCR(droplet digital PCR,ddPCR)检测方法。方法以新型冠状病毒BA.1株基因组为靶基因,选取刺突蛋白保守序列设计引物和探针,建立重组新型冠状病毒疫苗病毒颗粒数ddPCR方法,并进行线性、准确度、专属性、重复性和耐用性等方法学验证。结果ddPCR的最佳退火温度是63℃,病毒颗粒数为5×10^(5)~2×10^(7) VP/ml时,线性和回收率良好,探针和引物特异性好,重复性和中间精密度变异系数(coefficient of variation,CV)都在10%以内,耐用性CV值在15%以内,并且与qPCR结果相比,CV值都在10%以内。结论本研究建立的ddPCR方法灵敏度高、稳定性好、特异性强,可用于重组新型冠状病毒疫苗(腺病毒载体)病毒颗粒数的定量检测。展开更多
基金supported by grants from the National Key Research and Development Program of China(2017YFD0500800)the earmarked fund for China Agriculture Research System(CARS-42-17)+1 种基金the Integration and Demonstration of Key Technologies for Goose Industrial Chain in Sichuan Province,China(2018NZ0005)the Sichuan Veterinary Medicine and Drug Innovation Group of China Agriculture Research System(SCCXTD-2020-18)。
文摘Recombinant virus-vectored vaccines are novel agents that can effectively activate specific and nonspecific immunity,are multivalent and multieffective,and have high safety ratings.Animal alphaherpesviruses have a large genome,contain multiple nonessential regions that do not affect viral replication and are capable of accepting the insertion of an exogenous gene and expressing the antigen protein.Furthermore,animal alphaherpesviruses have a wide host spectrum,can replicate in the host and continuously stimulate the animal to produce immunity to the corresponding pathogen,thus making them ideal carriers for recombinant virus-vectored vaccines.With the development of gene-editing technology,recombinant viruses capable of expressing foreign genes can be constructed by various methods.Currently,studies on recombinant virusvectored vaccines constructed based on animal alphaherpesviruses have involved poultry,pigs,cattle,sheep,and companion animals.Studies have shown that the construction of recombinant animal alphaherpesviruses enables the acquisition of immunity to multiple diseases.This article mainly summarizes the current progress on animal alphaherpesvirus-vectored vaccines,aiming to provide reference for the development of new animal alphaherpesvirus-vectored vaccines.
文摘目的构建一种重组新型冠状病毒疫苗(腺病毒载体)病毒颗粒数微滴式数字PCR(droplet digital PCR,ddPCR)检测方法。方法以新型冠状病毒BA.1株基因组为靶基因,选取刺突蛋白保守序列设计引物和探针,建立重组新型冠状病毒疫苗病毒颗粒数ddPCR方法,并进行线性、准确度、专属性、重复性和耐用性等方法学验证。结果ddPCR的最佳退火温度是63℃,病毒颗粒数为5×10^(5)~2×10^(7) VP/ml时,线性和回收率良好,探针和引物特异性好,重复性和中间精密度变异系数(coefficient of variation,CV)都在10%以内,耐用性CV值在15%以内,并且与qPCR结果相比,CV值都在10%以内。结论本研究建立的ddPCR方法灵敏度高、稳定性好、特异性强,可用于重组新型冠状病毒疫苗(腺病毒载体)病毒颗粒数的定量检测。
