针对面向智能电网的业务网络传输问题,通过软件定义网络（Software Defined Networking,SDN）的方式不可避免的要处理数据种类繁杂且匹配流表过程中存在较高处理时延,为满足最高优先级数据的时延需求以及相对较低优先级数据的公平性,通...针对面向智能电网的业务网络传输问题,通过软件定义网络（Software Defined Networking,SDN）的方式不可避免的要处理数据种类繁杂且匹配流表过程中存在较高处理时延,为满足最高优先级数据的时延需求以及相对较低优先级数据的公平性,通过SDN的虚拟化技术及向量交换技术提出虚拟快速传输网络（Virtual Rapid Transmission Network,VRTN）,并用OPNET进行仿真。仿真结果证明VRTN能够有效的提高数据的传输效率,为SDN在智能电网中的应用研究提供有价值的参考。展开更多
为探索椎骨发育相关(Vertebrae Development Associated,VRTN)基因和核受体亚家族6A组成员1(Nuclear Receptor Subfamily 6 Group A Member 1,NR6A1)基因多态性与北京黑猪群体脊椎数的关联性,筛选脊椎数相关的重要分子标记,本研究利用PC...为探索椎骨发育相关(Vertebrae Development Associated,VRTN)基因和核受体亚家族6A组成员1(Nuclear Receptor Subfamily 6 Group A Member 1,NR6A1)基因多态性与北京黑猪群体脊椎数的关联性,筛选脊椎数相关的重要分子标记,本研究利用PCR技术和测序技术检测北京黑猪与胸椎数相关的重要候选基因VRTN和NR6A1的单核苷酸多态性(SNPs)位点,开展群体遗传学分析,并与胸椎数和腰椎数进行关联分析。结果显示:VRTN g.20311;0312ins291突变位点在北京黑猪群体中表现为ins/ins、ins/-、-/-3种基因型;g.19034A>C突变位点在北京黑猪群体中表现为AA、AC和CC3种基因型;NR6A1c.748C-T突变位点在北京黑猪群体中仅表现为TT基因型;关联分析结果表明,VRTN g.20311;0312ins291位点与北京黑猪的胸椎数和腰椎数无显著相关;g.19034A>C位点在北京黑猪中野生型个体(AA)的胸椎数显著低于突变纯合个体(CC),突变杂合个体(AC)的胸椎数与野生型个体和突变纯合个体差异均不显著;野生型个体(AA)的腰椎数显著高于突变纯合个体(CC),突变杂合个体(AC)的腰椎数与野生型个体和突变纯合个体的腰椎数差异均不显著。综上,VRTN基因g.19034A>C位点可作为潜在的北京黑猪多胸椎数分子标记。展开更多
Despite one SINE retrotransposon insertion polymorphism(sRTIP)in the vertebrae development-associated(VRTN)gene was identified in pigs,the structural variations(SVs)in VRTN gene and its proximal flank regions were lar...Despite one SINE retrotransposon insertion polymorphism(sRTIP)in the vertebrae development-associated(VRTN)gene was identified in pigs,the structural variations(SVs)in VRTN gene and its proximal flank regions were largely unknown.VRTN genic and flanking sequences from 14 breeds were assembled or downloaded from whole genome shotgun contings(WGS)database,and aligned to identify the SVs with Clustalx,and retrotransposons in VRTN gene were annotated by RepeatMasker,the splicing patterns of VRTN gene were predicted by Genescan,and large SVs were evaluated by PCR.A total of 12 small SVs and three large SVs in intron of VRTN,derived from SINE insertion polymorphisms.were identifed,and two of them(VRTN-sRTIP2 and VRTN-sRTIP3)were not reported before.These VRTN-sRTIPs may affect the splicing patterns of VRTN.They displayed polymorphisms in most detected eight breeds.VRTN-sRTIP2 and VRTN-sRTIP3 showed Hardy-Weinberg equilibrium distributions in most populations except the Chinese local Erhualian pigs,while VRTN-sRTIP1 showed genetic equilbrium in Erhualian pigs.Three VRTN-sRTIPs were identified,and displayed polymorphisms in pigs,and two of them were not reported before.These SVs provide a useful molecular markers for genetic analysis in pigs,and offer new information to facilitate the understanding the SVs of VRTN gene and their putative roles in the variation of vertebral number.展开更多
旨在研究VRTN(vertebrae development homolog)基因在巴马香猪群体中的编码区序列特征、组织表达情况、脊椎数性状因果突变位点ins291的等位基因频率及其与乳头数和产仔数性状的关联。本研究采集3头0日龄巴马香猪组织,利用cDNA克隆技术...旨在研究VRTN(vertebrae development homolog)基因在巴马香猪群体中的编码区序列特征、组织表达情况、脊椎数性状因果突变位点ins291的等位基因频率及其与乳头数和产仔数性状的关联。本研究采集3头0日龄巴马香猪组织,利用cDNA克隆技术获得VRTN基因编码区全长序列并进行生物信息学分析,利用荧光定量PCR技术检测VRTN在心、肝、脾、肺、肾、背最长肌和皮下脂肪组织中的表达情况;采集279头经产巴马香猪母猪血液,检测ins291位点在该群体中的频率分布,并与乳头数、产仔数性状进行关联分析。结果表明,巴马香猪VRTN基因编码区全长2 097 bp,其编码的氨基酸序列在不同物种间存在大量保守区域;VRTN基因编码698个氨基酸,预测为亲水性蛋白质,存在1个螺旋转角螺旋域超家族结构功能区、2个低复杂度区域和2个内部重复结构,并与核受体辅抑制子1(NR6A1)、果蝇同源框基因Prospero的脊椎动物同源蛋白2(PROX2)和富亮氨酸重复序列74亚基(LRRC74A)等蛋白具有相互作用;VRTN基因在0日龄巴马香猪各组织中均有表达,其中在背最长肌组织中表达量最高;巴马香猪保种群体中VRTN基因有利突变位点ins291的等位基因频率为21.15%,不同基因型个体之间平均产仔数、总乳头数、左侧乳头数、右侧乳头数和单侧最大乳头数性状均无显著差异(P>0.05),但纯合突变型(ins/ins)个体的乳头数性状均高于其他基因型个体。结果提示,在巴马香猪产业化生产中可通过分子育种手段提高VRTN ins291有利等位基因频率,但不影响其产仔数性状。展开更多
椎体发育同源物(vertebrae development homolog,VRTN)基因g.20311_20312ins291位点和黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因g.Asp298Asn位点分别是已报道的影响猪脊椎数和脂肪沉积性状的关键突变位点,但上述位点在不同...椎体发育同源物(vertebrae development homolog,VRTN)基因g.20311_20312ins291位点和黑素皮质素受体-4(melanocortin-4 receptor,MC4R)基因g.Asp298Asn位点分别是已报道的影响猪脊椎数和脂肪沉积性状的关键突变位点,但上述位点在不同种群对生长和胴体性状的影响不一,也未见将两位点进行组合研究。因此,本试验采用PCR扩增和PCR-RFLP方法对399头150日龄的美系大白猪群体VRTN基因g.20311_20312ins291位点和MC4R基因g.Asp298Asn位点的多态性进行检测,并分析其与生长和胴体性状的关联性。多态性分析结果表明,VRTN基因g.20311_20312ins291位点在大白猪群体中有Ins/Ins、Ins/Del和Del/Del 3种基因型,基因型频率分别为0.165、0.509和0.326;MC4R基因Asp298Asn位点在大白猪群体中存在GG、AG和AA 3种基因型,基因型频率分别为0.040、0.213、0.747。性状关联分析结果显示,VRTN基因g.20311_20312ins291位点对眼肌面积、瘦肉率有显著影响(P<0.05),对其他性状无显著影响(P>0.05);MC4R基因Asp298Asn位点对体重、日增重、背膘厚、眼肌面积及瘦肉率等性状均有显著影响(P<0.05)。合并基因型对体重、日增重、背膘厚及瘦肉率等4个性状有显著影响(P<0.05),对眼肌面积无显著影响(P>0.05);携带MC4R A等位基因的个体,除Del/Del-AG组合基因型外,无论VRTN是何种基因型,体重和日增重均能表现显著优势(P<0.05),其中Ins/Ins-AA基因型组合最佳。综上可知,VRTN和MC4R基因对大白猪生长和胴体性状具有一定影响,可为后续应用VRTN和MC4R基因辅助选育大白猪提供参考。展开更多
旨在探讨生殖细胞核因子(nuclear receptor subfamily 6 group A member 1,NR6A1)和椎体发育同源物(vertebrae development homolog, VRTN)基因的多态性对苏姜猪体重、体长、体高、胸围、胸宽、臀宽和背膘厚7个生产性状的影响。试验利用...旨在探讨生殖细胞核因子(nuclear receptor subfamily 6 group A member 1,NR6A1)和椎体发育同源物(vertebrae development homolog, VRTN)基因的多态性对苏姜猪体重、体长、体高、胸围、胸宽、臀宽和背膘厚7个生产性状的影响。试验利用PCR扩增和酶切片段多态性技术检测苏姜猪及其亲本猪品种中NR6A1和VRTN基因的多态性,并将NR6A1和VRTN基因的多态性与苏姜猪的生产性状进行关联性分析。遗传多态性分析结果表明,NR6A1基因p.Pro192Leu (g.299084751 C>T)位点在姜曲海猪、枫泾猪和杜洛克猪群体中已经纯合,分别为CC、CC和TT基因型;在苏姜猪群体中有TT、TC和CC 3种基因型,基因型频率分别为0.397、0.482和0.121,基因型分布频率符合哈迪温伯格平衡(Hardy-Weinberg equilibrium, HWE,P>0.05),处于中度多态(0.250.05),VRTN基因对苏姜猪胸围、胸宽和臀宽均有显著影响(P<0.05),对其他性状无显著影响(P>0.05)。合并基因型对苏姜猪体长、胸围和背膘厚均有显著影响(P<0.05),对其他性状无显著影响(P>0.05)。综上可知,NR6A1和VRTN基因对苏姜猪的生产性状具有一定影响,为后续应用NR6A1和VRTN基因辅助选育苏姜猪提供参考。展开更多
文摘为探索椎骨发育相关(Vertebrae Development Associated,VRTN)基因和核受体亚家族6A组成员1(Nuclear Receptor Subfamily 6 Group A Member 1,NR6A1)基因多态性与北京黑猪群体脊椎数的关联性,筛选脊椎数相关的重要分子标记,本研究利用PCR技术和测序技术检测北京黑猪与胸椎数相关的重要候选基因VRTN和NR6A1的单核苷酸多态性(SNPs)位点,开展群体遗传学分析,并与胸椎数和腰椎数进行关联分析。结果显示:VRTN g.20311;0312ins291突变位点在北京黑猪群体中表现为ins/ins、ins/-、-/-3种基因型;g.19034A>C突变位点在北京黑猪群体中表现为AA、AC和CC3种基因型;NR6A1c.748C-T突变位点在北京黑猪群体中仅表现为TT基因型;关联分析结果表明,VRTN g.20311;0312ins291位点与北京黑猪的胸椎数和腰椎数无显著相关;g.19034A>C位点在北京黑猪中野生型个体(AA)的胸椎数显著低于突变纯合个体(CC),突变杂合个体(AC)的胸椎数与野生型个体和突变纯合个体差异均不显著;野生型个体(AA)的腰椎数显著高于突变纯合个体(CC),突变杂合个体(AC)的腰椎数与野生型个体和突变纯合个体的腰椎数差异均不显著。综上,VRTN基因g.19034A>C位点可作为潜在的北京黑猪多胸椎数分子标记。
基金Supported by the National Natural Science Foundation of China(31872977 and 31572364)the Jiangsu Agricultural Science and Technology Innovation Fund(CX(19)2016)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China.
文摘Despite one SINE retrotransposon insertion polymorphism(sRTIP)in the vertebrae development-associated(VRTN)gene was identified in pigs,the structural variations(SVs)in VRTN gene and its proximal flank regions were largely unknown.VRTN genic and flanking sequences from 14 breeds were assembled or downloaded from whole genome shotgun contings(WGS)database,and aligned to identify the SVs with Clustalx,and retrotransposons in VRTN gene were annotated by RepeatMasker,the splicing patterns of VRTN gene were predicted by Genescan,and large SVs were evaluated by PCR.A total of 12 small SVs and three large SVs in intron of VRTN,derived from SINE insertion polymorphisms.were identifed,and two of them(VRTN-sRTIP2 and VRTN-sRTIP3)were not reported before.These VRTN-sRTIPs may affect the splicing patterns of VRTN.They displayed polymorphisms in most detected eight breeds.VRTN-sRTIP2 and VRTN-sRTIP3 showed Hardy-Weinberg equilibrium distributions in most populations except the Chinese local Erhualian pigs,while VRTN-sRTIP1 showed genetic equilbrium in Erhualian pigs.Three VRTN-sRTIPs were identified,and displayed polymorphisms in pigs,and two of them were not reported before.These SVs provide a useful molecular markers for genetic analysis in pigs,and offer new information to facilitate the understanding the SVs of VRTN gene and their putative roles in the variation of vertebral number.
文摘旨在探讨生殖细胞核因子(nuclear receptor subfamily 6 group A member 1,NR6A1)和椎体发育同源物(vertebrae development homolog, VRTN)基因的多态性对苏姜猪体重、体长、体高、胸围、胸宽、臀宽和背膘厚7个生产性状的影响。试验利用PCR扩增和酶切片段多态性技术检测苏姜猪及其亲本猪品种中NR6A1和VRTN基因的多态性,并将NR6A1和VRTN基因的多态性与苏姜猪的生产性状进行关联性分析。遗传多态性分析结果表明,NR6A1基因p.Pro192Leu (g.299084751 C>T)位点在姜曲海猪、枫泾猪和杜洛克猪群体中已经纯合,分别为CC、CC和TT基因型;在苏姜猪群体中有TT、TC和CC 3种基因型,基因型频率分别为0.397、0.482和0.121,基因型分布频率符合哈迪温伯格平衡(Hardy-Weinberg equilibrium, HWE,P>0.05),处于中度多态(0.250.05),VRTN基因对苏姜猪胸围、胸宽和臀宽均有显著影响(P<0.05),对其他性状无显著影响(P>0.05)。合并基因型对苏姜猪体长、胸围和背膘厚均有显著影响(P<0.05),对其他性状无显著影响(P>0.05)。综上可知,NR6A1和VRTN基因对苏姜猪的生产性状具有一定影响,为后续应用NR6A1和VRTN基因辅助选育苏姜猪提供参考。
