According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac effica...According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids.展开更多
Rapid emergence and a vigorous growth prevent the seed tubers from infections by soil microbes and allow a rapid interception of solar radiation. In this work, the effect of the potassium phosphites (KPhi) applied to ...Rapid emergence and a vigorous growth prevent the seed tubers from infections by soil microbes and allow a rapid interception of solar radiation. In this work, the effect of the potassium phosphites (KPhi) applied to seed tubers of two potato cultivars on crop emergence and early growth was studied. Two experiments were performed under greenhouse and field conditions. Emergence of plants, leaf area, dry matter and the number of primary stems were measured in both experiments. Furthermore, mycorrhizal colonization was also measured on roots under field conditions. The application of KPhi reduced the period between planting and emergence, and increased leaf area and dry matter. The ratio between dry matter of aerials and underground organs was not affected by KPhi. Indigenous mycorrhizal colonization increased after KPhi application to seed tubers. These results confirm the benefit of the application of KPhi to seed tubers on early plant growth and suggest that their application in crop production would be advantageous.展开更多
This study aimed to assess the microbiological quality of tiger nut (Cyperus esculentus L.) products sold in Ouagadougou and Bobo Dioulasso. The microbial load of tubers and tiger nut milk was determined using ISO 483...This study aimed to assess the microbiological quality of tiger nut (Cyperus esculentus L.) products sold in Ouagadougou and Bobo Dioulasso. The microbial load of tubers and tiger nut milk was determined using ISO 4833-1 (2003) standard (total mesophilic aerobic flora), NF ISO 7954 (1998) standard (yeasts and molds), and ISO 4832 (2006) standard (total coliforms, thermotolerant coliforms). The results showed that tiger nut tubers had an average microbial load between 4.86 ± 7.03 × 10<sup>6</sup> UFC/g and 1.82 ± 2.30 × 10<sup>7</sup> UFC/g for total mesophilic aerobic flora, from 4.34 ± 10.6 × 10<sup>5</sup> UFC/g to 3.52 ± 4.54 × 10<sup>6</sup> UFC/g for yeasts and molds, from 2.23 ± 2.25 × 10<sup>5</sup> UFC/g to 1.41 ± 2.16 × 10<sup>5</sup> UFC/g for total coliforms and from 1.83 ± 2.03 × 10<sup>5</sup> UFC/g to 7.0 ± 10.8 × 10<sup>4</sup> CFU/g for thermotolerant coliforms. For tiger nut milk samples, the average microbial load varied from 3.48 ± 2.98 × 10<sup>6</sup> CFU/g to 2.80 ± 5.69 × 107 CFU/g for total mesophilic aerobic flora, from 5.00 ± 7.21 × 10<sup>4</sup> CFU/g to 1.88 ± 3.31 × 10<sup>5</sup> CFU/g for yeasts and molds, from 4.58 ± 10.4 × 10<sup>4</sup> CFU/g to 6.31 ± 9.17 × 10<sup>5</sup> CFU/g for total coliforms and 7.00 ± 7.00 × 10<sup>3</sup> CFU/g to 2.87 ± 5.86 × 10<sup>5</sup> CFU/g for thermotolerant coliforms. This study revealed that the tubers and tiger nut milk sold in Ouagadougou and Bobo Dioulasso had a high microbial load which could lead to the degradation of these products and food poisoning for consumers.展开更多
This study aimed to evaluate the nutritional and sensory characteristics of penne-type pasta produced from locally sourced cereals, tubers, and legumes. To achieve this, we formulated four different types of pasta wit...This study aimed to evaluate the nutritional and sensory characteristics of penne-type pasta produced from locally sourced cereals, tubers, and legumes. To achieve this, we formulated four different types of pasta with varying levels of incorporation ranging from 10 to 50% cereals such as millet, fonio, and rice, tubers such as manioc and sweet potato, and legumes such as cowpea. The results showed that the incorporation of local products considerably improved the nutrient content of penne without being rejected by consumers. Sensory analysis showed that the best results were obtained with mixtures of sweet potato, cowpea, and wheat, as well as with rice, manioc, cowpea, and sample wheat. These results are of great importance to manufacturers in a world where developing nutritious and attractive food products is a crucial challenge.展开更多
The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam culti...The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam cultivars, including five from Dioscorea alata (cDa083, cDa053, cDa115, cDa150 and cDa266) and five from Dioscorea cayenensis-rotundata (cDr015, cDr027, cDr150, cDr206 and cDr148) with distinct agro-morphological characteristics, were used as material. A completely randomized block design with 3 replications was used. In each block, all cultivars and seed types were represented. When put into cultivation, the tubers produced by the vitroplants all germinated and gave 100% healthy plants, compared with 86% to 100% healthy plants for the seed tubers produced by the farmers. Yields per hectare ranged from 10 to 25 tonnes for seed tubers produced by in vitro plants, and from 4 to 9 tons for seed tubers produced by farmers. Seed tubers from vitroplants can therefore be recommended to farmers as a solution to the problem of seed tuber unavailability.展开更多
Exploring and evaluating alternative feed ingredients to be used in swine diet is essential due to highly variable cost and limited availability of conventional feed ingredients. Tubers and agro-industrial coproducts ...Exploring and evaluating alternative feed ingredients to be used in swine diet is essential due to highly variable cost and limited availability of conventional feed ingredients. Tubers and agro-industrial coproducts could provide the basis for producing affordable swine feed. However, information on the nutritional value of these potential alternative feedstuffs is necessary while considering their use in swine feeding program. Four tubers(purple sweet potato [PSP], okinawan sweet potato, taro and cassava) and 3 coproducts(okara, wheat millrun [WMR] and barley brewers grain [BBG]) were analyzed for their proximate nutrients, starch, fibers and gross energy(GE) content. Two independent in vitro studies were carried out for tubers and coproducts to determine their nutrients digestibility using a 3-step enzymatic assay(which mimics the digestion occurring in the gastrointestinal tract of swine) with 9 replicates of each sample digested in 3 batches equally. All replicate samples were used to determine in vitro dry matter digestibility(IVDDM) while 2 replicates from each batch were used to determine in vitro GE digestibility(IVDGE). Among tubers, CP content was the highest in taro(8.8%) and the lowest in cassava(3.7%), while CP content among coproducts was the highest in okara(22.7%) and the lowest in WMR(11.8%). Ether extract content among tubers ranged from 1.1% to 2.8%. The GE content among tubers, ranged from 4,134 to 4,334 kcal/kg whereas among coproducts it ranged from 4,270 to 4,794 kcal/kg. Among tubers, IVDDM for PSP was significantly higher(86.8%, P < 0.001) than taro(70.3%). Among coproducts, IVDDM of okara(74.1%) was significantly higher(P < 0.05) than BBG(61.3%). In conclusion,both tubers and coproducts can be used as a partial substitute of conventional energy feedstuffs in swine diets as these are rich in GE and other nutrients and are fairly digestible.展开更多
基金financially supported by the National Natural Science Foundation of China (81573396)Military Innovation Funding (16CXZ012)
文摘According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids.
文摘Rapid emergence and a vigorous growth prevent the seed tubers from infections by soil microbes and allow a rapid interception of solar radiation. In this work, the effect of the potassium phosphites (KPhi) applied to seed tubers of two potato cultivars on crop emergence and early growth was studied. Two experiments were performed under greenhouse and field conditions. Emergence of plants, leaf area, dry matter and the number of primary stems were measured in both experiments. Furthermore, mycorrhizal colonization was also measured on roots under field conditions. The application of KPhi reduced the period between planting and emergence, and increased leaf area and dry matter. The ratio between dry matter of aerials and underground organs was not affected by KPhi. Indigenous mycorrhizal colonization increased after KPhi application to seed tubers. These results confirm the benefit of the application of KPhi to seed tubers on early plant growth and suggest that their application in crop production would be advantageous.
文摘This study aimed to assess the microbiological quality of tiger nut (Cyperus esculentus L.) products sold in Ouagadougou and Bobo Dioulasso. The microbial load of tubers and tiger nut milk was determined using ISO 4833-1 (2003) standard (total mesophilic aerobic flora), NF ISO 7954 (1998) standard (yeasts and molds), and ISO 4832 (2006) standard (total coliforms, thermotolerant coliforms). The results showed that tiger nut tubers had an average microbial load between 4.86 ± 7.03 × 10<sup>6</sup> UFC/g and 1.82 ± 2.30 × 10<sup>7</sup> UFC/g for total mesophilic aerobic flora, from 4.34 ± 10.6 × 10<sup>5</sup> UFC/g to 3.52 ± 4.54 × 10<sup>6</sup> UFC/g for yeasts and molds, from 2.23 ± 2.25 × 10<sup>5</sup> UFC/g to 1.41 ± 2.16 × 10<sup>5</sup> UFC/g for total coliforms and from 1.83 ± 2.03 × 10<sup>5</sup> UFC/g to 7.0 ± 10.8 × 10<sup>4</sup> CFU/g for thermotolerant coliforms. For tiger nut milk samples, the average microbial load varied from 3.48 ± 2.98 × 10<sup>6</sup> CFU/g to 2.80 ± 5.69 × 107 CFU/g for total mesophilic aerobic flora, from 5.00 ± 7.21 × 10<sup>4</sup> CFU/g to 1.88 ± 3.31 × 10<sup>5</sup> CFU/g for yeasts and molds, from 4.58 ± 10.4 × 10<sup>4</sup> CFU/g to 6.31 ± 9.17 × 10<sup>5</sup> CFU/g for total coliforms and 7.00 ± 7.00 × 10<sup>3</sup> CFU/g to 2.87 ± 5.86 × 10<sup>5</sup> CFU/g for thermotolerant coliforms. This study revealed that the tubers and tiger nut milk sold in Ouagadougou and Bobo Dioulasso had a high microbial load which could lead to the degradation of these products and food poisoning for consumers.
文摘This study aimed to evaluate the nutritional and sensory characteristics of penne-type pasta produced from locally sourced cereals, tubers, and legumes. To achieve this, we formulated four different types of pasta with varying levels of incorporation ranging from 10 to 50% cereals such as millet, fonio, and rice, tubers such as manioc and sweet potato, and legumes such as cowpea. The results showed that the incorporation of local products considerably improved the nutrient content of penne without being rejected by consumers. Sensory analysis showed that the best results were obtained with mixtures of sweet potato, cowpea, and wheat, as well as with rice, manioc, cowpea, and sample wheat. These results are of great importance to manufacturers in a world where developing nutritious and attractive food products is a crucial challenge.
文摘The aim of this study is to produce large quantities of yam tubers in the field from seed tubers derived from vitroplants regenerated from aerial stem cuttings and farmers’ tuber seeds. Seed tubers from ten yam cultivars, including five from Dioscorea alata (cDa083, cDa053, cDa115, cDa150 and cDa266) and five from Dioscorea cayenensis-rotundata (cDr015, cDr027, cDr150, cDr206 and cDr148) with distinct agro-morphological characteristics, were used as material. A completely randomized block design with 3 replications was used. In each block, all cultivars and seed types were represented. When put into cultivation, the tubers produced by the vitroplants all germinated and gave 100% healthy plants, compared with 86% to 100% healthy plants for the seed tubers produced by the farmers. Yields per hectare ranged from 10 to 25 tonnes for seed tubers produced by in vitro plants, and from 4 to 9 tons for seed tubers produced by farmers. Seed tubers from vitroplants can therefore be recommended to farmers as a solution to the problem of seed tuber unavailability.
基金supported by the USDA, National Institute for Food and Agriculture, Hatch Project HAW02030-H, managed by the College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa
文摘Exploring and evaluating alternative feed ingredients to be used in swine diet is essential due to highly variable cost and limited availability of conventional feed ingredients. Tubers and agro-industrial coproducts could provide the basis for producing affordable swine feed. However, information on the nutritional value of these potential alternative feedstuffs is necessary while considering their use in swine feeding program. Four tubers(purple sweet potato [PSP], okinawan sweet potato, taro and cassava) and 3 coproducts(okara, wheat millrun [WMR] and barley brewers grain [BBG]) were analyzed for their proximate nutrients, starch, fibers and gross energy(GE) content. Two independent in vitro studies were carried out for tubers and coproducts to determine their nutrients digestibility using a 3-step enzymatic assay(which mimics the digestion occurring in the gastrointestinal tract of swine) with 9 replicates of each sample digested in 3 batches equally. All replicate samples were used to determine in vitro dry matter digestibility(IVDDM) while 2 replicates from each batch were used to determine in vitro GE digestibility(IVDGE). Among tubers, CP content was the highest in taro(8.8%) and the lowest in cassava(3.7%), while CP content among coproducts was the highest in okara(22.7%) and the lowest in WMR(11.8%). Ether extract content among tubers ranged from 1.1% to 2.8%. The GE content among tubers, ranged from 4,134 to 4,334 kcal/kg whereas among coproducts it ranged from 4,270 to 4,794 kcal/kg. Among tubers, IVDDM for PSP was significantly higher(86.8%, P < 0.001) than taro(70.3%). Among coproducts, IVDDM of okara(74.1%) was significantly higher(P < 0.05) than BBG(61.3%). In conclusion,both tubers and coproducts can be used as a partial substitute of conventional energy feedstuffs in swine diets as these are rich in GE and other nutrients and are fairly digestible.
