To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG PSN) and thymopeptides on T lymphocytes of normal and immunosuppressed mice, CD4 + and CD8 + T lymphocyte subsets of...To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG PSN) and thymopeptides on T lymphocytes of normal and immunosuppressed mice, CD4 + and CD8 + T lymphocyte subsets of single nucleic cell in thymus, spleen and peripheral blood were detected successively by flow cytometry after application of BCG PSN and thymopeptides. Meanwhile, CD4 +/CD8 + ratio was also calculated. The results showed that both BCG PSN and thymopeptides could decrease the proportion of CD4 + CD8 + T lymphocyte subsets in the thymus, at the same time increase CD4 + T lymphocyte, CD8 +T lymphocyte proportion in the three tissues. The fluctuation in amplitude was greater in thymopeptides group than that in BCG PSN group. It is concluded that acting location of thymopeptides is in thymus, its stimulating action is stronger than that of BCG PSN, while BCG PSN not only accelerates the differentiation in thymus, but also has some direct stimulation to peripheral CD4 +T lymphocytes, and can maintain CD4 +/CD8 + ratio within normal range. So, BCG PSN is safer.展开更多
目的:观察沙利度胺联合胸腺肽对系统性红斑狼疮(systemic lupus erythematosus,SLE)患者免疫功能及Th1/Th2平衡的影响。方法:选取2020年6月-2021年3月中国人民解放军联勤保障部队第九二三医院皮肤科收治的90例SLE患者,采用随机数字表法...目的:观察沙利度胺联合胸腺肽对系统性红斑狼疮(systemic lupus erythematosus,SLE)患者免疫功能及Th1/Th2平衡的影响。方法:选取2020年6月-2021年3月中国人民解放军联勤保障部队第九二三医院皮肤科收治的90例SLE患者,采用随机数字表法将其分为对照组和观察组,每组45例。对照组口服沙利度胺片,观察组在对照组的基础上服用胸腺肽肠溶片。比较两组患者的疗效、治疗指标、免疫功能及Th1/Th2细胞因子平衡,并记录研究期间两组患者发生的不良反应。结果:治疗后,观察组总有效率为93.33%,高于对照组的77.78%(P<0.05)。治疗后,两组血清肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白定量(urine protein quantification,UTP)和SLE疾病活动度指数(SLEDAI)评分均低于治疗前,而补体C3和C4均高于治疗前,观察组Scr、BUN、24 h UTP和SLEDAI评分均低于对照组,而补体C3和C4均高于对照组(P<0.05)。治疗后,两组CD4^(+)、CD4^(+)/CD8^(+)均高于治疗前,且观察组均高于对照组(P<0.05)。治疗后,两组干扰素-γ(IFN-γ)和IFN-γ/白细胞介素-10(IL-10)均高于治疗前,而IL-10均低于治疗前,观察组IFN-γ和IFN-γ/IL-10均高于对照组,而IL-10低于对照组(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:胸腺肽辅助沙利度胺可显著提高治疗SLE的效果,患者的肾功能、补体C3、C4水平和SLEDAI评分均显著改善,其机制可能与改善机体免疫异常和Th1/Th2平衡失调有关。展开更多
AIM: To improve the absorption of thymopeptides(TH) by preparing sodium deoxycholate/phospholipid-mixed nanomicelles(SDC/PL-MMs). METHODS: TH-SDC/PL-MMs were prepared by a film dispersion method, and then evaluated us...AIM: To improve the absorption of thymopeptides(TH) by preparing sodium deoxycholate/phospholipid-mixed nanomicelles(SDC/PL-MMs). METHODS: TH-SDC/PL-MMs were prepared by a film dispersion method, and then evaluated using photon correlation spectroscopy(PCS), zeta potential measurement, as well as their physical stability after storage for several days. Furthermore, in situ intestinal single-pass perfusion experiments and pharmacodynamics in immunodeficient mice were performed to make a comparison with TH powders and the control drug in absorption properties. RESULTS: A narrow size distribution of nanomicelles, with a mean particle size of(149 ± 8.32) nm and a zeta potential of(-31.05 ± 2.52) mV, was obtained. The in situ intestine perfusion experiments demonstrated a significant advantage in absorption characteristics for TH compared to the other formulations, and oral administration of TH-SDC/PL-MMs potentiated an equivalent effect with i.h. TH in pharmacodynamic studies in immunodeficient mice. CONCLUSIONS: TH-SDC/PL-MMs prepared by a film dispersion method are able to improve the absorption of TH. SDC/PL-MMs might be a good approach for the more effective delivery of drugs like TH.展开更多
Objective: To study the effect of Thymosin Injection combined with entecavir therapy on the viral load, liver fibrosis and immune response of patients with compensated liver cirrhosis. Methods: Patients who were diagn...Objective: To study the effect of Thymosin Injection combined with entecavir therapy on the viral load, liver fibrosis and immune response of patients with compensated liver cirrhosis. Methods: Patients who were diagnosed with chronic hepatitis b complicated by compensated liver cirrhosis in our hospital between March 2015 and January 2017 were selected and randomly divided into two groups, observation group received Thymosin Injection combined with entecavir therapy, and control group received entecavir therapy. The viral load, liver function injury indexes, liver fibrosis indexes, immune cytokines and immune cell apoptosis molecules were measured 24 weeks after treatment. Results: 24 weeks after treatment, HBV-DNA copy number in peripheral blood as well as ALT, AST, TBIL, HA, PC-III, LN, C-IV, IL-4, IL-10, TGF-β1, IL-17 and IL-22 levels in serum of observation group was significantly lower than those of control group, and peripheral blood FAS, CYCS, CASP8, CASP9 and CASP3 mRNA expression were significantly lower than those of control group. Conclusion:Thymosin Injection combined with entecavir therapy can reduce viral load, improve liver function, inhibit liver fibrosis and regulate immune function in patients with compensated liver cirrhosis.展开更多
文摘To compare the effects of polysaccharide nucleic acid fraction of bacillus calmette guerin (BCG PSN) and thymopeptides on T lymphocytes of normal and immunosuppressed mice, CD4 + and CD8 + T lymphocyte subsets of single nucleic cell in thymus, spleen and peripheral blood were detected successively by flow cytometry after application of BCG PSN and thymopeptides. Meanwhile, CD4 +/CD8 + ratio was also calculated. The results showed that both BCG PSN and thymopeptides could decrease the proportion of CD4 + CD8 + T lymphocyte subsets in the thymus, at the same time increase CD4 + T lymphocyte, CD8 +T lymphocyte proportion in the three tissues. The fluctuation in amplitude was greater in thymopeptides group than that in BCG PSN group. It is concluded that acting location of thymopeptides is in thymus, its stimulating action is stronger than that of BCG PSN, while BCG PSN not only accelerates the differentiation in thymus, but also has some direct stimulation to peripheral CD4 +T lymphocytes, and can maintain CD4 +/CD8 + ratio within normal range. So, BCG PSN is safer.
文摘目的:观察沙利度胺联合胸腺肽对系统性红斑狼疮(systemic lupus erythematosus,SLE)患者免疫功能及Th1/Th2平衡的影响。方法:选取2020年6月-2021年3月中国人民解放军联勤保障部队第九二三医院皮肤科收治的90例SLE患者,采用随机数字表法将其分为对照组和观察组,每组45例。对照组口服沙利度胺片,观察组在对照组的基础上服用胸腺肽肠溶片。比较两组患者的疗效、治疗指标、免疫功能及Th1/Th2细胞因子平衡,并记录研究期间两组患者发生的不良反应。结果:治疗后,观察组总有效率为93.33%,高于对照组的77.78%(P<0.05)。治疗后,两组血清肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白定量(urine protein quantification,UTP)和SLE疾病活动度指数(SLEDAI)评分均低于治疗前,而补体C3和C4均高于治疗前,观察组Scr、BUN、24 h UTP和SLEDAI评分均低于对照组,而补体C3和C4均高于对照组(P<0.05)。治疗后,两组CD4^(+)、CD4^(+)/CD8^(+)均高于治疗前,且观察组均高于对照组(P<0.05)。治疗后,两组干扰素-γ(IFN-γ)和IFN-γ/白细胞介素-10(IL-10)均高于治疗前,而IL-10均低于治疗前,观察组IFN-γ和IFN-γ/IL-10均高于对照组,而IL-10低于对照组(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:胸腺肽辅助沙利度胺可显著提高治疗SLE的效果,患者的肾功能、补体C3、C4水平和SLEDAI评分均显著改善,其机制可能与改善机体免疫异常和Th1/Th2平衡失调有关。
基金supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(No.20101561)Beijing Natural Science Foundation of China(No.7122176)
文摘AIM: To improve the absorption of thymopeptides(TH) by preparing sodium deoxycholate/phospholipid-mixed nanomicelles(SDC/PL-MMs). METHODS: TH-SDC/PL-MMs were prepared by a film dispersion method, and then evaluated using photon correlation spectroscopy(PCS), zeta potential measurement, as well as their physical stability after storage for several days. Furthermore, in situ intestinal single-pass perfusion experiments and pharmacodynamics in immunodeficient mice were performed to make a comparison with TH powders and the control drug in absorption properties. RESULTS: A narrow size distribution of nanomicelles, with a mean particle size of(149 ± 8.32) nm and a zeta potential of(-31.05 ± 2.52) mV, was obtained. The in situ intestine perfusion experiments demonstrated a significant advantage in absorption characteristics for TH compared to the other formulations, and oral administration of TH-SDC/PL-MMs potentiated an equivalent effect with i.h. TH in pharmacodynamic studies in immunodeficient mice. CONCLUSIONS: TH-SDC/PL-MMs prepared by a film dispersion method are able to improve the absorption of TH. SDC/PL-MMs might be a good approach for the more effective delivery of drugs like TH.
文摘Objective: To study the effect of Thymosin Injection combined with entecavir therapy on the viral load, liver fibrosis and immune response of patients with compensated liver cirrhosis. Methods: Patients who were diagnosed with chronic hepatitis b complicated by compensated liver cirrhosis in our hospital between March 2015 and January 2017 were selected and randomly divided into two groups, observation group received Thymosin Injection combined with entecavir therapy, and control group received entecavir therapy. The viral load, liver function injury indexes, liver fibrosis indexes, immune cytokines and immune cell apoptosis molecules were measured 24 weeks after treatment. Results: 24 weeks after treatment, HBV-DNA copy number in peripheral blood as well as ALT, AST, TBIL, HA, PC-III, LN, C-IV, IL-4, IL-10, TGF-β1, IL-17 and IL-22 levels in serum of observation group was significantly lower than those of control group, and peripheral blood FAS, CYCS, CASP8, CASP9 and CASP3 mRNA expression were significantly lower than those of control group. Conclusion:Thymosin Injection combined with entecavir therapy can reduce viral load, improve liver function, inhibit liver fibrosis and regulate immune function in patients with compensated liver cirrhosis.
