Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of e...Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of expression of TSLP in nasal epithelial cells （NECs） of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR （n=22） and nonallergic controls （n=11） during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale （VAS）. In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization （ISH） was performed to test the messenger RNA （mRNA） of TSLP in the NECs. Furthermore, immunohistochemical staining （IHC） was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group （P 〈0.05）. In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score （r=0.598; P 〈0.05） and nasal eosinophils count （r=0.702; P 〈0.05）, but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local 展开更多
Bamboo salt(BS) is a traditional Korean food, and has been reported to have anti-cancer, anti-inflammatory, and anti-metastatic effects. However, the anti-atopic dermatitis(AD) activity of BS has not been described ye...Bamboo salt(BS) is a traditional Korean food, and has been reported to have anti-cancer, anti-inflammatory, and anti-metastatic effects. However, the anti-atopic dermatitis(AD) activity of BS has not been described yet. In the present study, we examined the preventive effect of BS on AD. The effect of oral administration of BS was tested in a 2, 4-dinitrofluorobenzene(DNFB)-induced AD animal model, by histological analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, caspase-1 assay, and Western blotting analysis. BS administration reduced the total clinical severity and scratching frequencies, compared with the AD group. In the serum of DNFB-induced AD mice, the levels of IgE, histamine, thymic stromal lymphopoietin(TSLP), interleukin(IL)-5, and IL-13 were significantly reduced by BS treatment. BS significantly reduced the protein and mRNA expression of TSLP, IL-6, and tumor necrosis factor-α in the AD skin lesions. BS markedly reduced the infiltration of inflammatory cells. Furthermore, the activation of caspase-1 was reduced by BS in the AD skin lesions. Our results suggested that BS should be considered as a candidate treatment for allergic inflammatory diseases including AD.展开更多
目的探讨与分析血清中信号转导子和转录激活子(signal transducers and activators of transcription,STAT)-6、半乳糖凝集素(Galectin)-9、胸腺基质淋巴细胞生成素(Thymic stromal lymphopoietin,TSLP)、微小RNA(miR)-31表达及炎症因...目的探讨与分析血清中信号转导子和转录激活子(signal transducers and activators of transcription,STAT)-6、半乳糖凝集素(Galectin)-9、胸腺基质淋巴细胞生成素(Thymic stromal lymphopoietin,TSLP)、微小RNA(miR)-31表达及炎症因子的变化在哮喘儿童中的诊断价值。方法采用随机对照研究,研究时间为2016年2月到2018年1月,选择在北京市大兴区人民医院诊治的哮喘患儿72例作为观察组,同期选择健康儿童72例作为对照组,检测两组血清中STAT-6、Galectin-9、TSLP、miR-31、炎症因子与全血miR-31含量,并进行相关性分析。结果观察组血清TNF-ɑ、IL-6、STAT-6、Galectin-9、TSLP含量和全血miR-31相对表达水平显著高于对照组(P<0.05)。治疗后肺功能指标FEV 1%和PEF%均高于治疗前,炎症因子及STAT-6、Galectin-9、TSLP、miR-31表达均低于治疗前(P<0.05)。在观察组患中,Pearson相关性分析显示血清TNF-ɑ、IL-6、STAT-6、miR-31、TSLP、miR-31含量与肺功能指标FEV 1%和PEF%呈显著负相关性,与哮喘病情等级正相关(P<0.05)。ROC曲线显示炎症因子TNF-α、IL-6/STAT-6、Galectin-9、TSLP、miR-31曲线下面积分别为0.922,0.947,0.918,0.927,0.936,0.937,炎症因子和STAT-6、Galectin-9、TSLP、miR-31检测儿童哮喘具很高的判断价值。结论哮喘患儿表现为血清STAT-6、Galectin-9、TSLP、miR-31含量显著升高,并伴随有炎症因子TNF-ɑ、IL-6的释放,与哮喘病情存在正相关性,可影响哮喘的发生。展开更多
文摘Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of expression of TSLP in nasal epithelial cells （NECs） of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR （n=22） and nonallergic controls （n=11） during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale （VAS）. In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization （ISH） was performed to test the messenger RNA （mRNA） of TSLP in the NECs. Furthermore, immunohistochemical staining （IHC） was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group （P 〈0.05）. In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score （r=0.598; P 〈0.05） and nasal eosinophils count （r=0.702; P 〈0.05）, but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education,Science and Technology(No.2015R1D1A1A01056607)
文摘Bamboo salt(BS) is a traditional Korean food, and has been reported to have anti-cancer, anti-inflammatory, and anti-metastatic effects. However, the anti-atopic dermatitis(AD) activity of BS has not been described yet. In the present study, we examined the preventive effect of BS on AD. The effect of oral administration of BS was tested in a 2, 4-dinitrofluorobenzene(DNFB)-induced AD animal model, by histological analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, caspase-1 assay, and Western blotting analysis. BS administration reduced the total clinical severity and scratching frequencies, compared with the AD group. In the serum of DNFB-induced AD mice, the levels of IgE, histamine, thymic stromal lymphopoietin(TSLP), interleukin(IL)-5, and IL-13 were significantly reduced by BS treatment. BS significantly reduced the protein and mRNA expression of TSLP, IL-6, and tumor necrosis factor-α in the AD skin lesions. BS markedly reduced the infiltration of inflammatory cells. Furthermore, the activation of caspase-1 was reduced by BS in the AD skin lesions. Our results suggested that BS should be considered as a candidate treatment for allergic inflammatory diseases including AD.
文摘目的探讨与分析血清中信号转导子和转录激活子(signal transducers and activators of transcription,STAT)-6、半乳糖凝集素(Galectin)-9、胸腺基质淋巴细胞生成素(Thymic stromal lymphopoietin,TSLP)、微小RNA(miR)-31表达及炎症因子的变化在哮喘儿童中的诊断价值。方法采用随机对照研究,研究时间为2016年2月到2018年1月,选择在北京市大兴区人民医院诊治的哮喘患儿72例作为观察组,同期选择健康儿童72例作为对照组,检测两组血清中STAT-6、Galectin-9、TSLP、miR-31、炎症因子与全血miR-31含量,并进行相关性分析。结果观察组血清TNF-ɑ、IL-6、STAT-6、Galectin-9、TSLP含量和全血miR-31相对表达水平显著高于对照组(P<0.05)。治疗后肺功能指标FEV 1%和PEF%均高于治疗前,炎症因子及STAT-6、Galectin-9、TSLP、miR-31表达均低于治疗前(P<0.05)。在观察组患中,Pearson相关性分析显示血清TNF-ɑ、IL-6、STAT-6、miR-31、TSLP、miR-31含量与肺功能指标FEV 1%和PEF%呈显著负相关性,与哮喘病情等级正相关(P<0.05)。ROC曲线显示炎症因子TNF-α、IL-6/STAT-6、Galectin-9、TSLP、miR-31曲线下面积分别为0.922,0.947,0.918,0.927,0.936,0.937,炎症因子和STAT-6、Galectin-9、TSLP、miR-31检测儿童哮喘具很高的判断价值。结论哮喘患儿表现为血清STAT-6、Galectin-9、TSLP、miR-31含量显著升高,并伴随有炎症因子TNF-ɑ、IL-6的释放,与哮喘病情存在正相关性,可影响哮喘的发生。
