Stem segments from diseased Paulownia tomentosaXP, fortunei and leaves from healthy control were transformed with the expression vector p438PRSI via Agrobacterium tumefaciens. The p438PRSI vector contained shiva-1 gen...Stem segments from diseased Paulownia tomentosaXP, fortunei and leaves from healthy control were transformed with the expression vector p438PRSI via Agrobacterium tumefaciens. The p438PRSI vector contained shiva-1 gene, which encodes an antibacterial peptide under the control of a CaMV35S promoter. The regenerated plants from transformed explants were planted in a greenhouse and nursery. PCR and Southern blotting analysis showed that the shiva-1 gene was successfully integrated into the Paulownia genome. Transcription of the integrated shiva-1 gene was confirmed by RT-PCR. Bioassay in the green house and phytoplasma DNA-dot blotting demonstrated that resistance to Paulownia witch＇s broom disease （PWB） increased significantly in shiva-l-transgenic Paulownia. Further investigations indicated that higher Shiva- 1 expression correlated with fewer phytoplasma and less symptoms in diseased transgenic Paulownia. Together, our findings strongly suggest that breeding shiva-1-Paulownia is an effective strategy to control PWB disease.展开更多
The Lady of Shalott is considered to be one of Alfred Lord Tennyson’s masterpieces.The poem conversely demonstrates art and life,the aesthetic and the political,are fully interwoven:the involvement in the social worl...The Lady of Shalott is considered to be one of Alfred Lord Tennyson’s masterpieces.The poem conversely demonstrates art and life,the aesthetic and the political,are fully interwoven:the involvement in the social world which is symbolically the destination of the Lady in the poem.The Lady who is the emblem of such an artwork can be ascribed qualities,for instance,self-containment,objectified otherness,removal from the fluidity of life,and participation in a higher order of existence,for which it is autonomy.Kermode points out that“the Romantic artwork often identifies itself with an emblematic image of autonomy and femininity”,inextricably interlinked.Her mirror shows her only“Shadows of the world”.The Lady’s web flies“Out”and floats“wide”when she“turns round”with“that desire of making an impression upon another mind”,when she seeks to address Lancelot.The mirror reconnects the Lady’s art to the real world beyond her window.Though the artist is unable to see it during the process of production,her artistic web,once out in a wider space than the tower and freed from the loom,is a true representation of outward things,the actions,and events of Camelot.This paper examines that the traditional referentiality of Tennyson’s‘The Lady of Shalott’can be better understood by supplementing the poet’s medieval sources,of little more than tangential relevance to the poem,with Homeric influences.The poem is similar to a fairytale,however,I have given an alternate reality of the Lady of Shallot in my version of a fairytale,where the characters remain the same but the fairytale takes a happy ending rather than a tragic death.My version is different from Tennyson’s since I believe the lady deserves to be jovial.Sir Lancelot was unaware of her fascination and feelings for him,as she could hardly express her feelings due to her imprisonment.She is so taken by him that she stops her work and looks at Camelot,though she risks the mysterious curse.The mirror breaks and her web magically floats ou展开更多
基金the National Natural Science Foundation of China，the President Foundation of the Chinese Academy of Sciences
文摘Stem segments from diseased Paulownia tomentosaXP, fortunei and leaves from healthy control were transformed with the expression vector p438PRSI via Agrobacterium tumefaciens. The p438PRSI vector contained shiva-1 gene, which encodes an antibacterial peptide under the control of a CaMV35S promoter. The regenerated plants from transformed explants were planted in a greenhouse and nursery. PCR and Southern blotting analysis showed that the shiva-1 gene was successfully integrated into the Paulownia genome. Transcription of the integrated shiva-1 gene was confirmed by RT-PCR. Bioassay in the green house and phytoplasma DNA-dot blotting demonstrated that resistance to Paulownia witch＇s broom disease （PWB） increased significantly in shiva-l-transgenic Paulownia. Further investigations indicated that higher Shiva- 1 expression correlated with fewer phytoplasma and less symptoms in diseased transgenic Paulownia. Together, our findings strongly suggest that breeding shiva-1-Paulownia is an effective strategy to control PWB disease.
文摘The Lady of Shalott is considered to be one of Alfred Lord Tennyson’s masterpieces.The poem conversely demonstrates art and life,the aesthetic and the political,are fully interwoven:the involvement in the social world which is symbolically the destination of the Lady in the poem.The Lady who is the emblem of such an artwork can be ascribed qualities,for instance,self-containment,objectified otherness,removal from the fluidity of life,and participation in a higher order of existence,for which it is autonomy.Kermode points out that“the Romantic artwork often identifies itself with an emblematic image of autonomy and femininity”,inextricably interlinked.Her mirror shows her only“Shadows of the world”.The Lady’s web flies“Out”and floats“wide”when she“turns round”with“that desire of making an impression upon another mind”,when she seeks to address Lancelot.The mirror reconnects the Lady’s art to the real world beyond her window.Though the artist is unable to see it during the process of production,her artistic web,once out in a wider space than the tower and freed from the loom,is a true representation of outward things,the actions,and events of Camelot.This paper examines that the traditional referentiality of Tennyson’s‘The Lady of Shalott’can be better understood by supplementing the poet’s medieval sources,of little more than tangential relevance to the poem,with Homeric influences.The poem is similar to a fairytale,however,I have given an alternate reality of the Lady of Shallot in my version of a fairytale,where the characters remain the same but the fairytale takes a happy ending rather than a tragic death.My version is different from Tennyson’s since I believe the lady deserves to be jovial.Sir Lancelot was unaware of her fascination and feelings for him,as she could hardly express her feelings due to her imprisonment.She is so taken by him that she stops her work and looks at Camelot,though she risks the mysterious curse.The mirror breaks and her web magically floats ou
