AIM: To determine the effect of tetrahydrocurcumin (THC) on tumor angiogenesis compared with curcumin (CUR) by using both in vitro and in vivo models of human hepatocellular carcinoma cell line (HepG2). METHODS: The 3...AIM: To determine the effect of tetrahydrocurcumin (THC) on tumor angiogenesis compared with curcumin (CUR) by using both in vitro and in vivo models of human hepatocellular carcinoma cell line (HepG2). METHODS: The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay was used for testing the anti-proliferating activities of CUR and THC. In male BALB/c nude mice, 2 × 106 human HepG2 cells were inoculated onto a dorsal skin-fold chamber. One day after HepG2 inoculation, the experimental groups were fed oral daily with CUR or THC (300 mg/kg or 3000 mg/kg). On d 7, 14 and 21, the tumor microvasculature was observed using fluorescence videomicroscopy and capillary vascularity (CV) was measured. RESULTS: Pathological angiogenic features including microvascular dilatation, tortuosity, and hyper-permeability were observed. CUR and THC could attenuate these pathologic features. In HepG2-groups, the CV were significantly increased on d 7 (52.43%), 14 (69.17%), and 21 (74.08%), as compared to controls (33.04%,P < 0.001). Treatment with CUR and THC resulted in significant decrease in the CV (P < 0.005 and P < 0.001, respectively). In particular, the anti-angiogenic effects of CUR and THC were dose-dependent manner. However, the beneficial effect of THC treatment than CUR was observed, in particular, from the 21 d CV (44.96% and 52.86%, P < 0.05). CONCLUSION: THC expressed its anti-angiogenesis without any cytotoxic activities to HepG2 cells even at the highest doses. It is suggested that anti-angiogenic properties of CUR and THC represent a common potential mechanism for their anti-cancer actions.展开更多
Liver injury has become a serious liver disease worldwide,and its incidence is increasing year by year,bringing a serious health burden to people in all countries.Tetrahydrocurcumin is an active metabolite of curcumin...Liver injury has become a serious liver disease worldwide,and its incidence is increasing year by year,bringing a serious health burden to people in all countries.Tetrahydrocurcumin is an active metabolite of curcumin,which has pharmacological effects such as antioxidant,anti-inflammatory,inhibition of apoptosis,anti-tumor,and anti-aging,and it can inhibit apoptosis in liver cells under the state of hepatic injury and reduce the level of oxidative stress and inflammation in liver tissues through the signaling pathways such as MAPK,PI3K/Akt,PPAR,AMPK,and Nrf2 to prevent and control liver injury.The purpose of preventing and controlling liver injury can be achieved.The active structure,pharmacological effects and application of tetrahydrocurcumin in liver injury were summarized by reviewing domestic and international literature,with a view to providing reference for further research on tetrahydrocurcumin in the field of liver injury.展开更多
基金The Thailand Research Fund, No. MRG4980032partially supported by The National Center for Genetic Engineering and Biotechnology, Thailand
文摘AIM: To determine the effect of tetrahydrocurcumin (THC) on tumor angiogenesis compared with curcumin (CUR) by using both in vitro and in vivo models of human hepatocellular carcinoma cell line (HepG2). METHODS: The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay was used for testing the anti-proliferating activities of CUR and THC. In male BALB/c nude mice, 2 × 106 human HepG2 cells were inoculated onto a dorsal skin-fold chamber. One day after HepG2 inoculation, the experimental groups were fed oral daily with CUR or THC (300 mg/kg or 3000 mg/kg). On d 7, 14 and 21, the tumor microvasculature was observed using fluorescence videomicroscopy and capillary vascularity (CV) was measured. RESULTS: Pathological angiogenic features including microvascular dilatation, tortuosity, and hyper-permeability were observed. CUR and THC could attenuate these pathologic features. In HepG2-groups, the CV were significantly increased on d 7 (52.43%), 14 (69.17%), and 21 (74.08%), as compared to controls (33.04%,P < 0.001). Treatment with CUR and THC resulted in significant decrease in the CV (P < 0.005 and P < 0.001, respectively). In particular, the anti-angiogenic effects of CUR and THC were dose-dependent manner. However, the beneficial effect of THC treatment than CUR was observed, in particular, from the 21 d CV (44.96% and 52.86%, P < 0.05). CONCLUSION: THC expressed its anti-angiogenesis without any cytotoxic activities to HepG2 cells even at the highest doses. It is suggested that anti-angiogenic properties of CUR and THC represent a common potential mechanism for their anti-cancer actions.
基金supported by Key Program of Natural Science Foundation of Sichuan Province of China(2023NSFSC0040).
文摘Liver injury has become a serious liver disease worldwide,and its incidence is increasing year by year,bringing a serious health burden to people in all countries.Tetrahydrocurcumin is an active metabolite of curcumin,which has pharmacological effects such as antioxidant,anti-inflammatory,inhibition of apoptosis,anti-tumor,and anti-aging,and it can inhibit apoptosis in liver cells under the state of hepatic injury and reduce the level of oxidative stress and inflammation in liver tissues through the signaling pathways such as MAPK,PI3K/Akt,PPAR,AMPK,and Nrf2 to prevent and control liver injury.The purpose of preventing and controlling liver injury can be achieved.The active structure,pharmacological effects and application of tetrahydrocurcumin in liver injury were summarized by reviewing domestic and international literature,with a view to providing reference for further research on tetrahydrocurcumin in the field of liver injury.
