[Objective]To study the effects of homologous chromosomes 7 （7A,7B and 7D） on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochem...[Objective]To study the effects of homologous chromosomes 7 （7A,7B and 7D） on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochemical means. [Method]The Triticum asetivum cultivar Chinese Spring wheat and nullisomic wheat （N7A,N7B and N7D） were planted in greenhouse. The photosynthetic indexes were determined at early filling stage. [Result]The photosynthetic rate （Pn）,stomatal conductance （Gs）,primary photochemical efficiency （Fv/Fm）,actual chemical efficiency of photosystem II （ФPS II） and apparent electron transfer rate of photosystem II （ETR） were significantly lower in the N7A and N7B than in the Chinese Spring （P0.05）. The photosynthetic rate and stomatal conductance was significantly lower in the N7D than in the Chinese Spring （P0.05）. The Fv/Fm,ФPS II,ETR of N7D were higher than that of the Chinese Spring without significant difference. [Conclusion]The homologous chromosomes 7A and 7B have positive effects on photosynthetic rate and have relationship with stomatal conductance and photoreaction （chlorophyll fluorescence parameters）. The homologous chromosome 7D has negative effects on photosynthetic rate,which is mainly related to stomatal conductance rather than photoreaction.展开更多
TaSC(Triticum asetivum L.salt-tolerance related gene,Gen Bank登录号为AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦cDNA表达文库...TaSC(Triticum asetivum L.salt-tolerance related gene,Gen Bank登录号为AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦cDNA表达文库中钓取互作蛋白质,筛选到一个编码小麦未知功能蛋白质的基因(GenBank登录号为AK336035),命名为TaSCIP1(TaSC interaction protein 1)。双分子荧光互补(BiFC)实验证实TaSCIP1与TaSC存在互作。该互作蛋白的分离有利于进一步研究TaSC基因的耐盐机制。展开更多
基金Supported by Excellent Young Academic Leaders Project of Shanxi Province~~
文摘[Objective]To study the effects of homologous chromosomes 7 （7A,7B and 7D） on wheat photosynthesis and provide theoretical basis for breeding high photosynthetic efficiency wheat by genetic,physiological and biochemical means. [Method]The Triticum asetivum cultivar Chinese Spring wheat and nullisomic wheat （N7A,N7B and N7D） were planted in greenhouse. The photosynthetic indexes were determined at early filling stage. [Result]The photosynthetic rate （Pn）,stomatal conductance （Gs）,primary photochemical efficiency （Fv/Fm）,actual chemical efficiency of photosystem II （ФPS II） and apparent electron transfer rate of photosystem II （ETR） were significantly lower in the N7A and N7B than in the Chinese Spring （P0.05）. The photosynthetic rate and stomatal conductance was significantly lower in the N7D than in the Chinese Spring （P0.05）. The Fv/Fm,ФPS II,ETR of N7D were higher than that of the Chinese Spring without significant difference. [Conclusion]The homologous chromosomes 7A and 7B have positive effects on photosynthetic rate and have relationship with stomatal conductance and photoreaction （chlorophyll fluorescence parameters）. The homologous chromosome 7D has negative effects on photosynthetic rate,which is mainly related to stomatal conductance rather than photoreaction.
文摘TaSC(Triticum asetivum L.salt-tolerance related gene,Gen Bank登录号为AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因。以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦cDNA表达文库中钓取互作蛋白质,筛选到一个编码小麦未知功能蛋白质的基因(GenBank登录号为AK336035),命名为TaSCIP1(TaSC interaction protein 1)。双分子荧光互补(BiFC)实验证实TaSCIP1与TaSC存在互作。该互作蛋白的分离有利于进一步研究TaSC基因的耐盐机制。
